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Results The serum antibody level reached to 0.71±0.08-0.77±0.06 after genetic vaccine pCD-HCV1(100 μg/mouse) were inoculated into the mice(n=12) three or four times while blank vector pCD-SRα1 could not induce the mice(n=8) to generate antibody response in same way.After the antibody levels in mice(n=8) immunized by pCD-HCV1 had ascended to peak value(0.71), there was no trend of descending during the following 18 weeks of detection(0.68±0.06-0.75±0.07). Specific fragment of HCV cDNA identified by polymerase chain reaction from DNA extracted from the muscles of the mice after pCD-HCV1 had beed inoculated three months.
结果 基因疫苗pCD-HCV1(100 μg/只)3-4次接种小鼠后(n=12),血清抗体水平达0.71±0.08-0.77±0.06,空载体pCD-SRα1免疫鼠血清抗体阴性;免疫鼠(n=8)抗体水平达高峰后(0.71),持续检测18周未见下降趋势(0.68±0.06-0.75±0.07);pCD-HCV1免疫小鼠3个月后,用聚合酶链反应仍可从肌肉组织中扩增出特异性HCV cDNA片段;免疫鼠PBMC对HCV合成肽CP9、基因重组抗原C、E1刺激后均出现增殖反应,刺激指数分别为4.07±1.58、3.88±0.70和3.69±1.30,与pCD-SRα1免疫鼠相比,差异有显著性(P.001)。
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It was found that the vaccines used did not cause the adverse reactions such as hypersusceptibility and changes of feed intakes, and that 2 times of vaccinations of the vaccines all effectively enhanced the antibody levels, respectively, in which the No.Ⅲ vaccine stimulated antibody as rapid as the first vaccination was done while the No. I and No.Ⅱvaccines did not result in the antibody by the first vaccination. It was concluded that the No.
结果发现,三种供试的高致病性蓝耳病灭活疫苗均未引起供试猪发生过敏反应和采食量变化等不良反应;三种供试灭活疫苗经2次免疫后,均能提高抗体水平,其中供试疫苗Ⅲ在首次免疫后即能有效产生抗体,而供试疫苗I和供试疫苗Ⅱ在首次免疫后不能有效产生抗体。
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The acute rejection probability is higher in patients with positive PRA; in other hand, the probability is lower in patients with negative PRA.
术前、术后群体反应性抗体阳性患者,术后发生急性排斥的概率较高;而术前、术后群体反应抗体阴性的患者发生急性排斥的概率较低。
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Also studied was aza Diels-Alder reaction catalyzed by polyclonal antibody. The bicyclic [2, 2, 2] heptene and the aimed immunogen were synthesized via the key intermolecular asymmetric aza Diels-Alder reaction. Then four polyclonal antibodies were obtained by immunizing rabbit.
在研究多克隆抗体催化氮杂Diels-Alder反应中,通过分子间不对称氮杂Diels—Alder反应为关键反应合成了双环[2,2,2]庚烯类半抗原和目标抗原;用目标抗原免疫动物兔后得到了4株多克隆抗体,经动力学测试表明:多抗Aza-Bsa-3#能催化氮杂Diels—Alder反应的进行。
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Of the patients tested using CDC technology, 17 (3.8%) had a panel reactive antibody higher than 10% and 8 patients (1.83%) had a PRA higher than 25% for class I antibodies. Class I sensitized patients, defined as patients with a PRA higher than 10%, had significantly lower survival rates than class I nonsensitized patients ( P =.0035). Class I nonsensitized patients had survival rates of 79.4% at 1 year posttransplant, 63.2% at 3 years, and 45.4% at 5 years. Sensitized patients had 1, 3, and 5 year survival rates of 64.7%, 28.8%, and 9.6%, respectively.
使用CDC检测的病患之中,有17位(3.8%)群体反应抗体第一类抗体高於10%、有8位(1.83%) PRA超过25%,第一类抗体敏感病患定义为PRA高於10%者,相较於第一类抗体不敏感病患、存活率显著降低(P =。0035);第一类抗体不敏感病患移植后一年之存活率为79.4%、3年存活率为63.2%、5年存活率为45.4%;第一类抗体敏感病患移植后一年、3年、5年之存活率分别为64.7%、28.8%、9.6%。
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Results The level of antibodies against monocytes (34.94%) were higher than that of the antibodies against lymphocytes (26.67%) in pretransplant patients. The level of antibodies in first time transplant patients (32.5%) was lower than that of antibodies in retransplant patients (59.7%). The patients with intensity of antibodies against monocytes PRA more than ≥50%only accounted for 1.87%, 10%MPRA50%for 17.62%and MPRA10%15.44%,respectively.Conclusion Before renal transplantation, the detection of MPRA level could predict the occurrence of rejection in renal transplantation.
结果 肾移植术前患者的抗单核细胞抗体水平较抗淋巴细胞抗体水平高,其百分比分别为34.94%和26.67%;而从患者初次和再次肾移植前的比较中可见,初次比再次产生的抗单核细胞抗体水平要低,其百分比分别为32.05%和59.7%;对于抗单核细胞抗体群体反应性抗体的强度,MPRA≥50%的只占1.87%,而50%10%以及MPRA结论在肾移植术前检测患者MPRA水平,对肾移植后的排斥反应能起到预测目的。
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The ELISA assay established with the crude antigen-specfic monoclonal antibodies could detect both of the clinical and environmental isolates of Aspergllius, while the other assay could only detect Aspergillus fumigatus of both clinical and environmental isolates.And no cross reaction with the cell culture of Penialllium marneffei and Candidas was observed with the two methods.
用mAbs-1建立的双抗体夹心ELISA法可检测19种常见曲霉株培养液;用特异性针对烟曲霉抗原单克降抗体(mAbs-2)建立的双抗体夹心ELISA法可特异性检测临床和环境分离株烟曲霉培养液;与其他曲霉株无交叉反应;2种双抗体夹心ELISA法与马尔尼菲氏青霉菌及念珠菌培养液均无交叉反应。
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objective to establish immunological methods specific for detecting antigens in different groups of monoclonal antibodies.methods indirect immnofluorescence assay was applied to identify specificity of the two groups of monoclonal antibodies prepared with crude antigen and recombinant antigen of aspergillus fumigatus,respectively.two different double monoclonal antibody sandwich elisa assays established with the two groups of antibodies were performed to detect antigents in the cell culture supermatants of 19 common species of aspergillus,penicillium marneffei,and 5 species of candidas.results the results of indirect immnofluorescence assay indicated that the monoclonal antibodies prepared with crude antigen of aspergillus fumigatus were specific for antigens in both clinical isolates and environmental isolates of aspergillus, whereas the other group of monoclonal antibodies was proved to be specific for aspergillus fumigatus of both clinical and environmental isolates.the elisa assay established with the crude antigen-specfic monoclonal antibodies could detect both of the clinical and environmental isolates of aspergllius, while the other assay could only detect aspergillus fumigatus of both clinical and environmental isolates.and no cross reaction with the cell culture of penialllium marneffei and candidas was observed with the two methods.conclusion the elisa assays can detect both of the clinical and environmental isolates of aspergillus,and differentiate aspergillus fumigatus from other species of aspergillus.
目的 用2组曲霉单克隆抗体建立特异性识别不同种类曲霉抗原的检测方法。方法采用天然烟曲霉抗原免疫,获得广谱针对曲霉抗原的单克隆抗体;采用重组烟曲霉抗原获得特异性针对烟曲霉抗原的单克隆抗体,用间接免疫荧光鉴定,并分别建立2种双抗体夹心elisa法,对19种常见的环境和临床分离曲霉株、马尔尼菲氏青霉菌及念珠菌培养液进行检测。结果间接免疫荧光显示,用天然烟曲霉抗原免疫获得的单克隆抗体(mabs-1)可广谱识别多种曲霉分离株,而重组烟曲霉抗原获得的单克降抗体(mabs-2)仅能特异性结合临床和环境分离的烟曲霉抗原。用mabs-1建立的双抗体夹心elisa法可检测19种常见曲霉株培养液;用特异性针对烟曲霉抗原单克降抗体(mabs-2)建立的双抗体夹心elisa法可特异性检测临床和环境分离株烟曲霉培养液;与其他曲霉株无交叉反应;2种双抗体夹心elisa法与马尔尼菲氏青霉菌及念珠菌培养液均无交叉反应。
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Methods ABO, Rh and PRA were studied in ten transplant candidates with one or two-hand defect and potential donors. HLA was confirmed by PRC in these people. Mixed leukocyte culture was performed to determine T-cell alloreactivity between donor and recipient. After surgery, immunosuppressants such as antithymocyte globins, FK506, mycophenolic acid and prednisone were used.
方法对准备接受同种异体手移植的10例单手或双手缺失准备接受异体手移植的患者和潜在的供者进行ABO血型和Rh血型、群体反应抗体检测及混合淋巴细胞培养试验,采用聚合酶链反应方法测定人类白细胞抗原。
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The invention provides a human McAb to tetanus toxin, being a human antibody, which consists of an antibody variable region and an antibody constant region or not, having the ability of neutralizing the tetanus toxin, with the variable region gene and protein sequence indicated as the sequence table 1. The invention is characterized in that the antibody can not induce obvious allergic reaction, has higher titer and longer effect without any animal virus pollution, and can be produced in an unlimited quantity, which also provides the relating biological functions, the testing methods, the manufacturing method and the application.
A 本发明提供一种人源抗破伤风毒素单克隆抗体,其为人源抗体,包括抗体可变区,该抗体包括或不包括抗体的恒定区,该抗体具有中和破伤风毒素的能力,抗体可变区的基因和蛋白质序列如序列表1所示;其具有不会诱发明显的过敏反应,有较高的效价且长效,产品无动物病毒污染,可以无限量地生产;本发明还提供了其生物功能、测定方法、生产制造方法及其应用。
- 更多网络解释与反应抗体相关的网络解释 [注:此内容来源于网络,仅供参考]
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agglutination:凝集反应
凝集反应(agglutination)一种血清学反应. 颗粒性抗原(完整的病原微生物或红细胞等)与相应抗体结合,在有电介质存在的条件下,经过一定时间,出现肉眼可见的凝集小块. 参与凝集反应的抗原称为凝集原,抗体称为凝集素. 可分为直接凝集反应和间接凝集反应两类.
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agglutination reaction:凝集反应
凝集反应(agglutination reaction)是颗粒性抗原与相应抗体结合后,在一定条件下,出现肉眼可见的凝集物,称为凝集反应.凝集反应包括直接凝集反应和间接凝集反应.用乳胶颗粒吸附绒毛膜促性腺激素(HCG)作为已知抗原,以HCG免疫血清作为已知抗体,
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atopy:特异反应性
中...过敏:过敏可以是体液(抗体)或者是细胞免疫机制介导的.在大多数情况下,可产生过敏反应的抗体属于IGE类,这些个体可以归类于患有IGE-介导的过敏反应.然而,并非所有的"特异反应性" (atopy) 个体都会发生与
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precipitation:沉淀反应
沉淀反应 (precipitation)一种血清学反应. 可溶性抗原与相应抗体结合,在有适量电介质存在下,经过一定时间,形成肉眼可见的沉淀物. 沉淀反应的抗原可以是多糖、蛋白质、类脂等. 同相应抗体比较,抗原的分子小,单位体积内含有的抗原量多,
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reactivity:反应原性
抗原通常具有两种性能:①免疫原性 免疫原性(immunogenicity):系指抗原能够刺激 免疫原性 机体免疫系统发生免疫应答,产生抗体和/或致敏淋巴细胞的性能;②免疫反应 免疫反应 指抗原能与相应抗体和/或致性(immunoreactivity)或反应原性(reactivity): 敏淋巴细胞特异性结合,
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anaphylactic reaction:过敏反应
最近发现有意义的现象是病人发生严重过敏反应(Anaphylactic reaction)和抗体发生似乎有关. 两者可以同时被发现,过敏性反应可以在先或后出现. 我们也发现一位3岁男童,注射PCC几次后发生过敏性休克反应. 过几个月来到台大医院发现有第九因子抗体.
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reagin:反应素
5.反应素 (Reagin) 与异位性(Atopy)有关的特殊抗体,是免疫球蛋白E(lgE). 6.补体(Complement) 系存在于正常血清中的一组具有酶活性的球蛋白多分子系统,它参与多种抗原抗体反应,引起细菌、红细胞等的溶解,并促进对细菌的吞噬等防御作用.
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reagin:反应抗体
reaggregation 重聚合 | reagin 反应抗体 | reagin 反应素
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reagin:反应抗体 反应素
reaggregationagent 再聚集剂 | reagin 反应抗体 反应素 | reaging 反复老化
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reagin antibodies:反应素抗体
反应素 reagin | 反应素抗体 reagin antibodies | 实际生态区位 realized niche