- 更多网络例句与卵质体相关的网络例句 [注:此内容来源于网络,仅供参考]
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Different DNA constructs were introduced into the decapsulated cysts of Artemia by electroporation under 2KV, 20 μsec pulse length, 20 number of pulses and a DNA concentration of 20μg/μl in a volume of 100μl.
不同的构筑质体经由电穿孔法,被导入经去壳处理后的丰年虾休眠卵中,在2千伏特、20微秒的脉冲波长度、20次的脉冲数和在100微升的体积中DNA浓度为每微升20微克的设定条件下。
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Result:The best prescription was : Lecithin: Sodium Cholate =8∶1,Lecithin:Proanthocyanidins =15∶1,sonde-type ultrasound time was 5 min,the entrapment efficiency of FNL was 67.13%,and the results revealed the FNLs were sphere like with the mean size of 168.3 nm,the mean Zeta potential was –86.6 mV.
结果:最佳处方和制备工艺条件为卵磷脂与胆酸钠的质量比为8∶1、卵磷脂与原花青素的质量比为15∶1、探头式超声5 min。包封率为67.13%,所得柔性纳米脂质体为类球形实体粒子,平均粒径168.3 nm,平均Zeta电位-86.6 mV。
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The porine is the polyembryony animal, many obtains the ovocyte from the porine ovary although but the maturity quite to be low; Because the ROSI technology is does not have to grow the mature sole round sperm cell to pour into directly completely in the ovicell nature, jumped over the spermatozoon in to pass through the physiology and the biochemistry, if the ovicell nature mature or the activation degree were insufficient, added the round immature sperm cell maturity quite inferior reason, very possibly Causes the ROSI micro fertilization defeat.
猪是多胎动物,从猪卵巢上获得的卵母细胞数量虽然较多,但成熟度比较低;由于ROSI技术是将没有完全发育成熟的单一圆形精细胞直接注入卵胞质内,跳越了精子在穿过透明带和卵质膜等过程中所发生的生理和生化反应;如果卵胞质成熟或活化程度不够,加之圆形未成熟精细胞成熟度比较差等原因,很可能造成ROSI受精的卵母细胞内部激活因子蓄积减少和生成不足,引起精核解聚困难、精圆核不能形成、第二极体不能排出、卵母细胞孤雌发育率增加等,使ROSI显微受精失败。
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Methods: Lecithin and the sucrose ester-phospholipids liposome were prepared using the ethyl ether injection method by mixing sucrose ester with lecithin, sphingomyelin and cholesterol according to different lipids ratio.
按不同比例将乙酸异丁酸蔗糖酯与卵磷脂、鞘磷脂和胆固醇混合,溶于乙醚中,然后注入pH7.2的PBS缓冲液中,制成卵磷脂脂质体及蔗糖酯-卵磷脂脂质体。
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Recombinant plasmid pSVH 7 DNA of avian influenza virus H7 subtype heamagglutinin gene was encapsulated with DC-chol/DOPE liposomes and PC/chol/SA liposomes separately. Two-week old SPF chickens were intramuscularly inoculated with 50 μ g/0.2ml of the liposome entrapped PSVH 7 DNA. Four-weeks later, each chicken was challenged with 0.1ml 〓 AIV . One week after the challenge, the secretion of the cloacas was collected and transfected to chicken embryos to isolate the virus. The virus was isolated from 6/6 of the control group, 1/6 of the naked DNA group, 1/6 of the PC/chol/SA entrapped DNA group and 0/6 of the DC-chol/DOPE liposome entrapped group. The HI antibody titers (log2) of the four groups were 6. 83±0.98, 7. 0±1. 26, 7. 83±1. 17 and 8. 00±0.89 respectively 1-week after challenge, and 8. 5±0.55, 8. 17±0.82, 8. 68±0.45 and 9. 33±0.54 respectively 2-week after challenge. The results showed that inoculation of liposome entrapped DNA significantly enhanced resistance to virosis in animals.
将含禽流感病毒H7亚型血凝素基因的重组质粒pSVH7用DC-chol阳离子脂质体和胆固醇/卵磷脂/十八胺脂质体包裹,免疫2周龄SPF鸡,4周后用同型禽流感病毒进行人工感染,1周后采集泄殖腔分泌物分离病毒,结果未免疫组6/6分离到病毒,裸质粒DNA免疫组1/6分离到病毒胆固醇/卵磷脂/十八胺脂质体包裹DNA免疫组1/6分离到病毒,DC-chol脂质体DNA免疫组没有分离到病毒(0/6):人工感染后1周各组的HI抗体(Log2)分别为6.38±0.98,7.00±1.26,7.83±1.17,8.00±0.89,2周后为8.50±0.55,8.67±0.82,8.68±0.45,9.33±0.52,脂质体包裹组在同期均高于未免疫组和裸DNA免疫组,表明脂质体包裹质粒DNA免疫动物后,能增加动物对病毒感染的抵抗力和反应能力。
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objective the effects of anthocyanin pigment from maize purple plant on resisting lipid peroxidation were investigated.methods the inhibition of anthocanin pigment from maize purple plant was examined in vitro that autcoxudation of lecithin liposome system induced by fe2+.50 mice were randomly divided into 5 groups.vehicle and different dose anthocyanin pigment from maize purple plant were given respectively,and then experimental injury of mice liver was induced to make use of bromobenzene and the content of mda was determined in liver homogenate.results the inhibition rates of anthocyanin pigment from maize purple plant on linoleic acid oxidation was higher that of ascorbic ascorbic acids.the content of mda in homogenate in middle and high dose of pigment were significantly lower than that in low dose and the injured control group.there were no significat differences in the content of mda in homogenate between low dose group and injury control group.conclusion the anthocyanin pigment from maize purple plant have the capability to resist lipid peroxide.
目的 探讨玉米紫色植株色素抗脂质过氧化的作用。方法体外实验测定玉米紫色植株花色苷色素在fe2+引发的卵磷脂脂质体体系中抗氧化活性。体内实验,取50只小鼠随机分成5组,分别给予溶媒和不同剂量的色素,然后采用溴代苯致实验性肝损伤,测定肝匀浆的丙二醛含量。结果在由fe2+引发的卵磷脂脂质体体系中玉米紫色植株色素对脂质过氧化有明显的抑制作用,抑制率随样品的浓度增高而增大,并且明显优于抗坏血酸。在溴代苯致小鼠实验性肝损伤模型实验中,中、高剂量组的丙二醛含量均低于损伤模型组。低剂量组和损伤模型组比较丙二醛含量差异无统计学意义。
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The effect of aluminum ions on the Fe2+-initiated lipid peroxidation in liposomes was studied by chemiluminescence, TBARS, conjugate dienes assays and ESR spectroscopy.
利用化学发光、TBA 反应与测量共轭二烯的方法观测了Al3+对Fe2+启动的卵磷脂脂质体脂质过氧化的影响。
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Including the soybean lecithin separation purification, gamma - the linolenic acid separation and the purification, as well as gamma - linolenic acid fat 质体 Bao He studies and so on, and carries on optimal using the orthogonal experiment to the above craft.
其中包括大豆卵磷脂的分离纯化,γ-亚麻酸的分离和纯化、以及γ-亚麻酸脂质体包合研究等等,并利用正交试验对上述工艺进行优选。
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East valuable Jin Danzhong the gamma - linolenic acid and the soybean lecithin will make the fat 质体 both to conform to the original group side principle and to be possible to increase the preparation the stability and the curative effect.
将东宝金丹中γ-亚麻酸和大豆卵磷脂制成脂质体既符合原组方原则又可增加制剂的稳定性和疗效。
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In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.
在本研究中我们在取得两种不同品系小鼠的卵丘卵母细胞复合体后,先将卵丘卵母细胞复合体置于含有多种生长因子和激素的MEM培养液中培养6小时,此时卵母细胞已进入第一次减数分裂的前中期,并且在DIC倒置显微镜下可以看到浓缩的染色体,用Polscope可以发现明显的纺锤体,借助这种显微镜通过显微操作将两种不同品系小鼠来源的卵母细胞的MI纺锤体进行互换,经过三次直流电脉冲作用后,分别有91.6%的胞质—MI核质体对融合,经过进一步的培养后,超过80%的重组卵母细胞排出第一极体,体外受精后分别有79%和77%的重组卵形成双原核,受精后的胚胎在KSOM胚胎培养液中体外培养4天后,超过60%的胚胎发育至囊胚。
- 更多网络解释与卵质体相关的网络解释 [注:此内容来源于网络,仅供参考]
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archiblast:卵质
archibenthic zone 深海底带 | archiblast 卵质 | archicarp 产囊体
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archiblast:卵浆/卵质
archibald /高射炮/ | archiblast /卵浆/卵质/ | archicarp /产囊体/
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archicarp:产囊体
archiblast 卵质 | archicarp 产囊体 | archimetaboly 原变态
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insemination:授精
按照术语学家的定义,授精( insemination) 使得精卵相遇,精卵质膜融合. 受精( fertilization)指的是精子和卵子各自的单倍体基因组相融合形成两倍体合子的事件. 对于那些在卵子完成减数分裂之前精子进入卵子的生物体而言,
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plasmodium:原质团
而是无细胞壁且形状可变的原质团(plasmodium)或具细胞壁的、卵圆形的单细胞. 寄生在植物上的真菌往往以菌丝体在寄主的细胞间或穿过细胞扩展蔓延. 当菌丝体与寄主细胞壁或原生质接触后,营养物质因渗透压的关系进入菌丝体内.
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ooplasm:卵浆/卵质
oophyte 卵体 | ooplasm 卵浆 卵质 | oosome 卵小体
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ovoplasm, ooplasm:卵质
04.0054 极体 polar body | 04.0055 卵质 ovoplasm, ooplasm | 04.0056 种质 germplasm
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ooplasmic segregation:卵质分离
ooplasm 卵质 | ooplasmic segregation 卵质分离 | oosome 卵小体
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ooplast:卵质体
06.092 卵质 ooplasm | 06.093 卵质体 ooplast | 06.094 [卵]周质 periplasm
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Stabilizers:穩定劑
膜脂卵磷脂脑磷脂(cephalin)鞘脂(带有一个氨基)糖脂(结合有寡糖链)膜脂磷脂胆固醇总膜脂的约5%以上含磷酸质脂含糖质脂胆固醇 脂质体:...鲜鸡蛋与蛋制品,由于其含有大量的卵磷脂,具有永久性乳化能力,因而也能起到乳化剂的作用.(六)稳定剂(Stabilizers)稳定剂又称安定剂,具有亲水性,