印迹

基本解释 (translations)

print  ·  prints  ·  neurogram

与印迹相关的网络例句 [注：此内容来源于网络，仅供参考]

Using the flexibility of proteins, egg white albumin was pore-formed and heat-denatured in the presence of substrate template. BSA protein and herring sperm DNA were trasted by precipitation method in organic solvent.

利用蛋白质在水溶液中的柔性，分别采用热变性法对蛋清蛋白质进行生物印迹和沉淀法对BSA进行生物印迹，并在BSA印迹的基础上又印迹了鲱鱼精脱氧核糖核酸。

In the preparation of bio-imprinted enzymes, two bio-imprinting approaches are available:(1) bio-imprinting of egg albumin using heat-denaturalization; and (2) bio-imprinting of egg albumin using bovine serum albumin using dissolving-precipitation process.

在制备生物印迹酶方面，利用蛋白质在水溶液中的柔性，分别采用两种方法对蛋白质进行生物印迹：以热变性法对蛋清蛋白进行生物印迹，以及用溶解沉淀法对牛血清蛋白进行生物印迹。

It comprises following steps: dissolving cross linker, functional monomer, mode molecule and initiator into pore-generating agent, getting mixing solvent; hypersonic de-gassing the mixing solvent; venting nitrogen after even mixing, sealing at nitrogen condition or vacuum condition; proceeding polymerization reaction, employing heat initiation for molecule engram polymer; grinding got molecule engram polymer after polymerization, sifting and removing mode molecule with organic solvent, checking with high efficiency liquid chromatography- ultraviolet until there is no mode molecule; vacuum drying and getting final product.

具体为：首先将交联剂、功能单体、模板分子和引发剂溶解在致孔剂中，得到混合溶剂；将混合溶剂超声脱气；混合均匀后，通入氮气，氮气状态或抽真空状态下密封；进行聚合反应，分子印迹聚合物采用热引发；聚合反应结束后，将合成的分子印迹聚合物取出研磨、过筛后用有机溶剂除去模板分子，通过高效液相色谱-紫外检测，直到检测不到模板分子；将除去模板分子的聚合物，真空干燥，得到利血平特异的分子印迹聚合物。

The thermodynamic behaviour in the separation of molecular imprinting was studied.

研究了分子印迹分离过程中的热力学行为，测定了萘普生和酮洛芬分子印迹分离过程中的的焓变、熵变和自由能变化，计算结果得出ΔH和ΔS的值均为负值，并且熵的变化很小，这说明萘普生和酮洛芬分子印迹拆分过程是受焓控制的，从而进一步说明影响分子印迹分离的两个主要因素中，与模板分子互补的功能基比空间孔穴占有更重要的作用。

This thesis gives a brief overview of the development of the principle of MIT, the preparation and application of molecularly imprinted polymers, and offers an insight into the problems met with and how the area is likely to evolve in the near future.

本文对分子印迹技术的基本原理、分子印迹聚合物的制备和应用进行了较为全面的综述，对分子印迹技术当前存在的问题和未来的发展趋势进行了分析和展望。

In chapter 1, in this review section, the principles of MIT, the preparation methods and the application of molecularly imprinted polymers were summarized. The current problems occurred in MIT and its development were analyzed and prospected.

第一章，文献综述；对分子印迹技术的基本原理、分子印迹聚合物的制备和应用进行了总述，对分子印迹技术当前存在的问题和未来的发展趋势进行了分析和展望。

A brief introduction of molecular imprinting technology and molecularly imprinted membrane is outlined.

文中首先简要介绍了分子印迹技术及分子印迹膜，继而对分子印迹膜的制备方法进行了评述，并对现有的分子印迹膜的应用及研究方向进行了介绍和展望。

The conclusions were as follows: theνO-H andνC=O IR absorption maxima shifted towards higher wave numbers after imprinting 17β-estradiol on TFMAA-co-TRIM copolymer with the red shift of theνO-H groups being apparent after precipitation polymerization for 16h while that forνC=O groups being observed after polymerization for 24h. A strong interaction between TFMAA and 17β-estradiol was confirmed by the high selectivity for 17β-estradiol, as indicted by the values of the separation factor of isomers of 17β-estradiol/17α-estradiol (2.28) and the imprinted factor (3.01). Particle diameter of TFMAA-co-TRIM polymer was between 300 nm and 1.5μm, which suited well for solid phase sorbent throughout at low column pressure. The recognition of imprinting TFMAA-co-TRIM polymer for sterol molecules was driven by enthalpy eluted with acetronitile, and low temperature was in favor of the separation of sterol structure analogues on imprinting column. TFMAA-co-TRIM polymer possessed of determinate anti-heat stability, with melting point beginning at 255.84℃, Tp=257.40℃, control TFMAA-co-TRIM polymer and imprinting TFMAA-co-TRIM polymer were respectively decompounded at 267.79℃and 343.11℃, and solid micro-extraction noddle prepared by the special polymerization also showed definite recognition for 17β-estradiol by GC/MS detecting at 270℃. By selection of various washing and elution solvents, elution reagents of close polymerization system were of more advantage in template molecules retention and recognition on molecularly imprinted solid phase extraction column. At a certain extent, progesterone, 17α-estradiol, 17β-estradiol and 4-androstene-3, 17-dione could be intercepted on the MISPE column. Especially, MISPE had high selectivity for progesterone, and imprinting TFMAA-co-TRIM polymer could achieve adsorption balance within 50 min by absorption kinetics test for 17β-estradiol. However, MISPE column showed better selectivity and enrichment property for 17β-estradiol than C18 and CSPE columns according to the data from HPLC and GC/MS analyses. Recovery of 17β-estradiol on MISPE column was up to 85.5% while when prime extracting solution of milk powder was sampled, the recovery of CSPE and C18 columns were 43.7% and 30.7%, respectively.

通过研究阐明：紫外聚合产物TFMAA-co-TRIM中的νO-H振动吸收峰在聚合16h后红移，νC=O振动吸收峰在聚合24h后红移；TFMAA-co-TRIM对雌二醇异构体的印迹因子达到了3.01，α为2.28，优于其它功能单体参与得到的聚合物识别特性；TFMAA-co-TRIM聚合物粒径介于300 nm至1.5μm之间，作为色谱固定相具有良好的通量和低的柱压；在乙腈流动相中，TFMAA-co-TRIM分子印迹固定相的分离过程主要被焓驱动，低温有利于分子印迹固定相分离甾醇结构类似物；TFMAA-co-TRIM分子印迹聚合物在255.84℃时开始熔融，Tp=257.40℃，聚合物CP的降解温度在267.79℃，MIP降解温度在343.11℃，制备的固相微萃取头初步经GC/MS 270℃的耐热性测定；不同的洗提溶剂筛选证明了选择接近聚合溶剂的洗脱体系更有利于MISPE发挥识别效应；分子印迹固相萃取柱对孕酮、17α-雌二醇、17β-雌二醇和雄烯二酮都具有不同程度的保留特性，可作为此类化合物的吸附材料，特别对孕酮强保留的富集特性，可作为孕酮的选择性识别吸附剂，其分子印迹聚合物对17β-雌二醇的吸附动力学测定显示在50 min内基本达到吸附平衡，具有作为传感器核心敏感材料的潜力；对比萃取奶粉中17β-雌二醇性能，MISPE柱比非分子印迹固相萃取柱和C18柱具有更高的保留，回收率依次为85.5%，43.7%和30.7%。

Trans-Blot Cells and Systems 170-3825 Trans-Blot Cell With Wire Electrodes and PowerPac HC Power Supply, 100120/220240 V, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm), power supply, power cord, instructions 170-3850 Trans-Blot System With Plate Electrodes and PowerPac HC Power Supply, 100120/220240 V 170-3853 Trans-Blot System With Plate Electrodes, Super Cooling Coil, and PowerPac HC Power Supply, 100120/220240 V, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm), power supply, power cord, instructions 170-3910 Trans-Blot Cell With Wire Electrodes, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) 170-3939 Trans-Blot Cell With Plate Electrodes and Super Cooling Coil, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) 170-3946 Trans-Blot Cell With Plate Electrodes, includes 2 gel holder cassettes, buffer tank, lid with power cables, 4 fiber pads, 1 pack precut blot absorbent filter paper (15 x 20 cm) Trans-Blot Cell Accessories 170-3912 Super Cooling Coil, required for all high-intensity transfers 170-3913 Gel Holder Cassette, includes 2 fiber pads 170-3914 Fiber Pads, 15.5 x 20.5 cm, 6 170-3920 Trans-Blot Standard Wire Electrode Card, cathode 170-3921 Trans-Blot Standard Wire Electrode Card, anode 170-3922 Trans-Blot Cell Buffer Tank 170-3923 Trans-Blot Cell Lid With Power Cables 170-3943 Trans-Blot Platinum Anode Plate Electrode 170-3944 Trans-Blot Stainless-Steel Cathode Plate Electrode 170-3945 Trans-Blot Plate Electrode Pair, platinum anode and stainless-steel cathode 16 规格:说明: Trans-Blot Plus

电泳转印槽组件 1。缓冲液槽及带有电缆的盖 2。凝胶支架转印夹 3。纤维衬垫 4。电极丝 5。电极板 6。特级冷却芯 Trans-Blot 转印槽是功能灵活的转印设备，可理想地用于多种转印应用。Trans-Blot 转印槽特点包括：*能进行多胶转印，可容纳3 块PROTEAN I xi 凝胶、6块Criterion 凝胶、12块Mini-PROTEAN 3 或Ready Gel 预制胶*多组参数灵活可设，可调节的电压设置（从30 V 的过夜转印到200 V 的1 小时快速实验）*电极间距设置为8 cm 用于标准印迹杂交，或设置为4cm 用于高强度印迹杂交*可选择板式电极：涂有铂金的钛作为正极，不锈钢为负极，能提供高强度电场和比其它电极更高的电流密度。或选择较经济的铂金电极丝*通过特级冷却芯和水循环仪来调节温度―是天然酶（4°C）或高强度转印的理想选择，随着转印时间增加（多达24 小时），不会引起缓冲液耗竭（在高强度转印中必须使用冷却芯，也推荐用于所有板式电极的应用）*带铰链的凝胶支架转印夹能避免滑动，确保凝胶与印迹膜间的紧密接触；每个转印夹都有颜色标记以保证在转印槽中的正确定位 Trans-Blot 转印槽的锁闭凝胶支架转印夹系统。转印夹（1）支撑凝胶（2）印迹膜（3）两侧有纤维衬垫和滤纸（4）确保凝胶三明治内的完全接触。凝胶夹垂直插入缓冲液槽中（5）。

Secondly,UV spectrophotometry was adopted to research the combination action on SM_2 and SIZ with MAA and 4-Vp.The combination action of functional monomer with template molecules were produced in the results. Then,the prediction imprinting principle of SM_2 and SIZ were given. The diagrams of infrared spectrum were analysed in MAA and 4-Vp and IMPs of SM_2. It was discoved that the absorbability peak of C=C function group in the IMPs were turn to be very low ,but the function group of carboxylic acid and pyridyl in the IMPs were no evidence change, and the condition was created in the molecular recognition of MIPs. Thermal Analysis was adopted to research IMPs ,in which ,a better thermal stability and its decompose temperature were showed in the results.

其次，本文利用紫外分光光度法研究磺胺二甲嘧啶和磺胺异噁唑与甲基丙烯酸或4-乙烯基吡啶的结合作用，结果表明，溶液中的功能单体与模板分子之间产生了结合作用，并预测磺胺二甲嘧啶和磺胺异噁唑印迹机理；分别分析了功能单体甲基丙烯酸、4-乙烯基吡啶、磺胺二甲嘧啶和磺胺异噁唑分子印迹聚合物的红外谱图，经过对比发现制得的印迹聚合物中C=C双键峰很小，并且功能键羧酸键和吡啶氮没有明显变化，这为聚合物特异识别特性创造了条件；利用综合热分析仪，对分子印迹聚合物进行综合热分析，结果表明分子印迹聚合物具有较好的热稳定性，分解温度也较高。

与印迹相关的网络解释 [注：此内容来源于网络，仅供参考]

dot blot：斑点印迹

反应产物因不溶于水、二甲苯和醇的棕色沉淀物而被广泛地用于蛋白印迹(Western Blot,WB)、免疫组织化学(Immunohistochemistry,IHC)和免疫细胞化学(Immunocytochemistry,ICC)、斑点印迹(Dot blot)和生物芯片(Bio

dot blot：斑点印迹,斑点印迹膜

dosage compensation 计量补偿(效应) | dot blot 斑点印迹,斑点印迹膜 | dot blotting 点渍法,斑点印迹(法)

blotter：印迹装置

blot transfer apparatus 印迹转移装置 | blotter 印迹装置 | blotting 印迹

genomic imprinting：基因组印迹

(三)甲基化与基因组印迹 基因组印迹(genomic imprinting):指基因表达活性只局限于来自双亲之一的基因版本. 被印迹(imprinted)的基因. 基因组印迹的机制--DNA 高度甲基化 基因组印记与肿瘤. 第二节 转录水平的调控 转录水平的调控是基因表达调控中最重要的环节.一,

molecular imprinting：分子印迹

分子印迹(molecular imprinting)是对目标化合物具有特定识别能力的一种聚合物制备技术.分子印迹聚合物具有物理性质和化学性质稳定、制备简单、成本低等优点,使其在色谱分离、传感器、模拟酶催化、膜分离及固相萃取等许多领域得到了广泛的应用.

molecular imprinting：分子印迹(技术)[原指采用特定印迹物来制备高分子聚合物的特异性

moity 组成成分 | molecular imprinting 分子印迹(技术)[原指采用特定印迹物来制备高分子聚合物的特异性 | molecular lung 分子肺[血红蛋白随氧的得失而发生的四级结构及亚基间距离的变化]

dot blotting：点渍法,斑点印迹(法)

dot blot 斑点印迹,斑点印迹膜 | dot blotting 点渍法,斑点印迹(法) | dot hybridization 斑点杂交

dot blotting：点渍法,葵二酸二辛脂印迹(法)

dot blot 斑点印迹,斑点印迹膜 | dot blotting 点渍法,葵二酸二辛脂印迹(法) | dot hybridization 斑点杂交

blotting, Southern：萨瑟恩印迹技术

blot 印迹 | blotting, Southern 萨瑟恩印迹技术 | blotting, vacuum 抽真空印迹技术

imprinted gene：印迹基因

具有这种现象的基因称为印迹基因(Imprinted Gene)(1). 基因组印迹已经成为外因遗传学(Epigenetics)理论的重要组成部分. 基因印迹在人类遗传性疾病尤其是肿瘤发生中的作用正引起越来越多的注意. 本文就基因印迹的研究现状以及与消化道肿瘤关系的研究进行综述.