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The survival time of these mice were observed. The count of white blood cells and the percentage of immature granulocyte and monocyte obtained from tail vein were determined on 1 day before transplanting WEHI-3 cells and 7, 14, 19, 24, 28 days after transplanting. Myeloblast and monoblast percentage of bone marrow were checked as soon as these mice died.
BALB/c小鼠分别经尾静脉和球后接种1×10^6 WEHI-3细胞，观测其生存天数，光镜监测BALB/c小鼠外周血白细胞计数、未成熟粒单核细胞百分率及骨髓原始粒单核细胞百分率，电镜观测其骨髓白血病细胞形态。
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Objective To study the mechanism of monocyte recruitment in atherogenesis and to clarify the effect of monocyte chemotactic protein-1 (MCP-1) in this process.
目的 了解动脉粥样硬化发病过程中单核细胞粘附并进入动脉内膜的机制，分析单核细胞趋化蛋白-1（MCP-1）基因表达对单核细胞粘附动脉内膜的作用。
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Results The level of antibodies against monocytes (34.94%) were higher than that of the antibodies against lymphocytes (26.67%) in pretransplant patients. The level of antibodies in first time transplant patients (32.5%) was lower than that of antibodies in retransplant patients (59.7%). The patients with intensity of antibodies against monocytes PRA more than ≥50% only accounted for 1.87%, 10%＜ MPRA ＜50% for 17.62% and MPRA ＜10% 15.44%,respectively.
结果 肾移植术前患者的抗单核细胞抗体水平较抗淋巴细胞抗体水平高，其百分比分别为34.94%和26.67%；而从患者初次和再次肾移植前的比较中可见，初次比再次产生的抗单核细胞抗体水平要低，其百分比分别为32.05%和59.7%；对于抗单核细胞抗体群体反应性抗体的强度，MPRA≥50%的只占1.87%，而50%＜MPRA＞10%以及MPRA＜10%的分别占17.62%和15.44%。
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Results The level of antibodies against monocytes (34.94%) were higher than that of the antibodies against lymphocytes (26.67%) in pretransplant patients. The level of antibodies in first time transplant patients (32.5%) was lower than that of antibodies in retransplant patients (59.7%). The patients with intensity of antibodies against monocytes PRA more than ≥50%only accounted for 1.87%, 10%MPRA50%for 17.62%and MPRA10%15.44%,respectively.Conclusion Before renal transplantation, the detection of MPRA level could predict the occurrence of rejection in renal transplantation.
结果 肾移植术前患者的抗单核细胞抗体水平较抗淋巴细胞抗体水平高，其百分比分别为34.94%和26.67%；而从患者初次和再次肾移植前的比较中可见，初次比再次产生的抗单核细胞抗体水平要低，其百分比分别为32.05%和59.7%；对于抗单核细胞抗体群体反应性抗体的强度，MPRA≥50%的只占1.87%，而50%10%以及MPRA结论在肾移植术前检测患者MPRA水平，对肾移植后的排斥反应能起到预测目的。
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These results indiacated that tranferring macrophages combinedwith cytokines could be a new adoptive immunotherapy protocol foradvanced cancer,and rTNF used in vivo locally could induce andactivate macrophages to kill tumor cells.Monocytes when activatedunderwent a series of phenotypic and functional changes includingthe expression of IL-2R which may provide an important immunoregu-latory pathway.The presence of lectin-like molecules on thesurface of monocytes and tumor cells may bring theeffector/target cells together,thus facilitating the inductionof apoptosis in target cells by triggering the production ofcytolytic factors and the modification of target of targer cell surface anti-gens.(such as HLA-DR).
综合以上结果可以得出以下结论：转输巨噬细胞并结合细胞因子的联合应用是肿瘤继承性免疫中的又一新的、有潜力的方法；抗肿瘤细胞因子rTNF可通过激活巨噬细胞在体内抑制对其不敏感的肿瘤细胞的生长；人单核细胞在一定条件下激活后，可表达IL-2R，进而一方面增强其自身对IL-2的敏感性，另一方面在体内也具有调节T细胞功能的作用；单核细胞和肿瘤细胞上存在的凝集素类受体可促使两种细胞之间在凝集素介导下相互接近，并诱发单核细胞产生细胞毒因子，以及通过调节靶细胞上表面抗原的表达，促进诱导靶细胞的细胞凋亡。
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D. The function of stimulating xenogenous lymphocyte proliferation was the same between peripheral DCs and ascites PCs. E. The percentage of CD3〓CD56〓 cells was the same in CIK cells co-culture with DCs transfected with SKOV3 RNA, CIK cells co-culture with DCs, and CIK cells. F. The expansion rate of CIK cells can be accelerated by co-culturing with loaded or unloaded DCs. However, the expansion rate between loaded or unloaded group is the same. F. The strongest cytotoxicity against SKOV3 cell line was achieved by CIK cells co-cultured with DCs loaded with SKOV3 lysate.
结果：1、腹水可获得0.83±0.24×10〓个AMC/ml，单核细胞有0.74±0.25×10〓个/ml；2、卵巢癌患者外周血可获得0.87±0.20×10〓个AMC/ml，单核细胞有0.92±0.17×10〓个/ml；3、除CD86外周血单核细胞来源DC表达较高以外，其他表面分子在不同来源DC间没有统计学差异；4、不同来源DC的异基因刺激能力没有差异；5、与负载或未负载卵巢癌抗原的DC共培养并不能提高CIK细胞群中CD3〓CD56〓细胞的数量；6、CIK细胞增殖显著，培养14天时可扩增19.18±4.70倍，培养21天时可扩增35.82±4.36倍；7、与未负载或负载DC共培养的CIK细胞在培养第14天后增殖速率大于单纯CIK细胞。
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However, fully consideration of special structure and physiological function of CRP is critical to investigate whether CRP provide protect effect or pathogenic effect in the progression of atherosclerosis. CRP is a member of pentrxins family, which consists of five identical, noncovalently associated 23-kDa protomers arranged symmetrically around a central pore with a 102 A external diameter. Each protomer has a recognition face with a phosphocholine binding site consisting of two coordinated calcium ions adjacent to a hydrophobic pocket. The opposite face of the pentamer is the effector face, where complement Clq binds and Fc receptors are presumed to bind. A cleft extends from the center of the protomer to the central pore of the pentamer. Both faces ofpentamer form a special bi-phase structure: one face is the ligand recognition phase, which can recognize apoptosis cells and enzyme modified low density lipoprotein in which PCh is exposed.
CRP是一种五聚体蛋白，外径为102 A，由5个相同的单体以非共价键结合，形成双面的环形结构，每个单体的配体识别位点或受体结合位点分别位于五聚体两个平面上，分别组成配体识别相和效应器相，从而构成特殊的二相性结构：一面为配体识别相，含有磷酸胆碱(phosphorycholine，PCh)结合位点，能够使CRP识别在病理条件下暴露出PCh的凋亡细胞、酶修饰低密度脂蛋白(enzyme modified low density lipoprotein，E-LDL)等。E-LDL是天然低密度脂蛋白(native Low density lipoprotein，N-LDL)在多种蛋白酶，如胰蛋白酶、神经氨酸酶、胆固醇酯酶等作用下的代谢产物，其主要特点是结构发生改变(N-LDL大小均匀，平均直径250±30 A，E-LDL大小差异极大，直径为100-2000 A)，暴露磷酸胆碱位点，能够被CRP识别；另一面为效应器相，能够与巨噬细胞、单核细胞表面受体FcγRⅡa结合，介导巨噬细胞、单核细胞识别及吞噬凋亡、坏死细胞作用。
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The model of ECV-304 cell oxidative stress injury was established by hydrogen peroxide (H2O2).Then EPZ-contained blood serum was taken as experimental drug. The adherence of monocytes to endothelial cell were measured by method of rose Bengal. The total RNA of cells was extracted. The intercellular adhesion molecule-1 (ICAM-1),vascular cell adhesion molecule-1 (VCAM-1) and MCP-1 mRNA expression in cells were detected by RT-PCR.MCP-1 protein expression were detected by ELISA.
采用过氧化氢建立离体培养的血管内皮细胞(ECV-304)氧化应激损伤模型，取健康大鼠每日灌服不同剂量的鲜姜有效部位(200，400，800 mg·kg-1)或阳性对照药洛伐他汀(40 mg·kg-1)，取含药血清作为受试药物，用孟加拉玫红染色法测定单核细胞与内皮细胞的黏附力；逆转录聚合酶链反应测定细胞内细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子(VCAM-1)以及单核细胞趋化蛋白(MCP-1)的mRNA表达水平；酶联免疫吸附法测定MCP-1蛋白表达水平。
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It is unclear that how A20 is ex- pressed in patients of CHD, T2DM and CHD with T2DM.Inflammatory cytokines secreted from actived monocytes/macrophages in instable plaque result in plaque rupture, and those mono- cytes/ma- crophages come from circulation.
不稳定斑块中激活的单核巨噬细胞分泌大量的炎症因子导致斑块破裂，斑块中的单核巨噬细胞主要来自血液循环，故循环中的单核细胞可反映斑块中单核细胞状态。
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An abnormal increase of monocytes in the blood, occurring in infectious mononucleosis and certain bacterial infections such as tuberculosis.
单核细胞增多症单核细胞在血液中异常地增多，出现在传染性单核细胞增多症和某些细菌感染中，如肺结核
- 更多网络解释与单核细胞相关的网络解释 [注：此内容来源于网络，仅供参考]
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Monocytic Leukemia：单核细胞白血病
单核细胞白血病(monocytic leukemia) 的特异性皮肤损害为紫色到红褐色丘疹,结节和斑块,急性单核细胞白血病(AMOL)可发生水疱性损害. 皮损发展周期快,可自然消退.
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Monocytic Leukemia：单核细胞性白血病
monocyte 单核细胞 | monocytic leukemia 单核细胞性白血病 | monocytic series 单核细胞系
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Monocytic Leukemia：单核细胞性血白病
单核细胞 monocyte | 单核细胞性血白病 monocytic leukemia | 单基因病 monogenic disease
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Mo：单核细胞
5,单核细胞(MO):在白细胞总数中约占2.5-10%. 临床意义:增高:某些感染如EB病毒感染、结核、伤寒、疟疾、沙门菌、弓形虫感染、亚急性心内膜炎、急性感染恢复期、粒缺恢复期、淋巴瘤、单核细胞白血病等. (二)红细胞数(RBC),
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monocyte：单核细胞
4.单核细胞 单核细胞(monocyte)占白细胞总数的3%~8%. 它是白细胞中体积最大的细胞. 直径14~20μm,呈圆形或椭圆形. 胞核形态多样,呈卵圆形、肾形、马蹄形或不规则形等. 核常偏位,染色质颗粒细而松散,故着色较浅. 胞质较多,呈弱嗜碱性,
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monocyte,Mon：单核细胞
monocyte chemotactic protein, MCP 单核细胞趋化蛋白 | monocyte,Mon 单核细胞 | mononuclear phagocyte system, 单核吞噬细胞系统
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Infectious Mononucleosis：传染性单核细胞增多症
传染性单核细胞增多症(infectious mononucleosis)是一种急性的单核-巨噬细胞系统增生性疾病 病程常具自限性. 临床上表现为不规则发热、淋巴结肿大 咽痛、周围血液单核细胞显著增多,并出现异常淋巴细胞 嗜异性凝集试验阳性,血清中可测得抗EB病毒的抗体等
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Infectious Mononucleosis：单核细胞增多症
[概述] 传染性单核细胞增多症(infectious mononucleosis)是一种急性的单核-巨噬细胞系统增生性疾病,病程常具自限性. 临床上表现为不规则发热、淋巴结肿大、咽痛、周围血液单核细胞显著增多,并出现异常淋巴细胞、嗜异性凝集试验阳性,
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monocytic angina：传染性单核细胞增多症
monocyte 单核白细胞,单核细胞 | monocytic angina 传染性单核细胞增多症 | monocytic leukemia 单核细胞性白血病
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monocytopoiesis：单核细胞发生
幼浆细胞 proplasmacyte | 单核细胞发生 monocytopoiesis | 原单核细胞 monoblast