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On the basis of the kinetic equation of substrate reaction in the presence of urea or guanidine hydrochloride, all microscopic kinetic constants for the free enzyme and enzyme-substrate binary and ternary complexes have been determined. The results of the present studies indicate that:①In the presence of urea or guanidine hydrochloride, enzyme-substrate complexes lose their activity less rapidly than the free enzyme. Therefore, both substrates, NADPH and 7, 8-dihydrofolate, protect dihydrofolate reductase against inactivation.②The denaturation of dihydrofolate reductase by urea follows single-phase kinetics, and changes in enzyme activity and tertiary structure proceed simultaneously in the unfolding process, so it may be an"all or none"process.③The GdnHCl-induced unfolding of the dihydrofolate shows a biphasic transition, while the change in the enzyme activity is a single exponential process. The rate constant of inactivation is consistent with that of the fast conformational change. Therefore, the kinetic intermediate of protein unfolding should be a partially folded and inactive form.
我们根据在脲或盐酸胍存在下的底物反应动力学方程求得游离酶和酶底物二元、三元复合物的微观动力学常数，结果表明：①酶-底物二元、三元复合物的失活速度明显慢于游离酶，说明两个底物二氢叶酸和NADPH对酶的失活都具有一定程度的保护作用；②在脲作用下，酶的失活和构象变化均为单指数项过程，而且酶的活力丧失和三级结构变化是同时发生的，说明二氢叶酸还原酶的脲变性可能是一个"全或无"的两态过程；③在盐酸胍作用下，酶的构象变化为两相过程，而失活则是单指数项过程，酶分子构象变化的快相速度常数与失活速度常数基本一致，因此我们认为二氢叶酸还原酶的盐酸胍变性过程中存在一个没有活力、但仍具备一定空间结构的变性中间体。
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The macular threshold and FM 100-hue test in 46 patients with normal vi- sion in one eye and suffering from AMD in the fellow eyes, and 46 age-matched normal subjects were observed with FM 100-hue test、Macular threshold program of Humphrey field analyzer and Amlser gride test.
八、单侧老年性黄斑变性对侧眼的黄斑阈值及FM100-色调试验检测采用FM100-色调试验仪、Humphrey视野分析仪黄斑阈值程序Amsler表对46例单侧AMD对侧眼和46只眼年龄相匹配的正常对照组进行检查。
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To observe the choriocapillaris closure during hematoporphyrin mono methyl ether photodynamic therapy in New Zealand rabbits in order to investigate whether HMME-PDT can be used to treat aging macular degeneration.
观察血卟啉单甲醚光动力疗法对家兔脉络膜毛细血管的封闭作用及对视网膜非选择性损伤的特点，探讨HMME是否能够选择性引起脉络膜毛细血管的闭合，达到治疗老年性黄斑变性的目的。
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We establish an asymptotic Lipschitz homotopy invariance theorem for these K-homology groups and K-theory groups. We show that the asymptotic index maps are isomorphisms for the asymptotically scaleable spaces, which include Euclidean cones, simply connected complete Riemannian manifolds with nonpositive curvature.
我们证明了这些K-同调群和K-理论群具有渐近Lipschitz同伦不变性；对于渐近可标度的几何空间（包括欧氏锥、单连通非正曲率完备黎曼流形等），证明了渐近指标映射为同构。
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This paper presented a new method for ranking the alternatives in AHP, different from the traditional methods, it can directly obtain the importance weight of each alternative by only solving alinear equations, without any given criteria or the paired comparison matrix. Moreover, it keeps the transitivity among the alternatives and the relative importance between arbitrary two neighbouring alternatives in the entire sequence ranked.
针对层次分析方法中传统的层次单排序方法存在的弊端，提出一种新的层次单排序方法，该方法不需判断矩阵和一致性检验，通过解线性方程组直接获得各因素关于上层因素的重要性权值，且保证了因素间重要程度的传递性以及相对重要程度的不变性。
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For it is rubor after damaging to the closing soft tissue, getting swollen, getting hot aching and model of functiolaesa these bed solicit and there is no fracture,no skin damaged, we designed the corresponding experiment, namely the law of hot board causes little mouse\'s painful experiment of threshold of pain; Freun\'s complete adjuvant caused the swollen experiment of big mouse\'s foot;The xylene caused the swollen experiment of little mouse\'s ear; Invigorating blood circulation silt experiment that changes the sex that the rheology appearance examines the blood and flows with the blood; Useing the high performance liquid chromatography-electrochemistry Detected monoamine neurotransmitters transmitter in the brain and serum; the experiment of immunohistochemistry measured the proto-oncogene C-FOS expresses.
针对闭合性软组织损伤后的红、肿、热、痛及功能障碍的典型临床体征，加上无骨折、无皮肤破损的特点，我们设计了相应的实验，即热板法致小鼠疼痛的痛阈实验；福氏完全佐剂(Freun\'s complete adjuvant，FCA)致大鼠足肿实验；二甲苯致小鼠耳肿实验；用血流变仪测血液流变性的活血化瘀实验；运用高效液相色谱—电化学(high performance liquid chromatography-electrochemistry Detection HPLC-ECD)法测量大脑及血清中单胺类神经递质的实验以及免疫组织化学法检测原癌基因C-FOS蛋白产物表达的实验。
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The relationship between a typical sigmoidal curve of denaturation and the process described by twostate model is analyzed in detail. It is shown that sigmoidal behavior does not mean that the unfolding process of the single-domain protein is two-state behavior.
通过对典型的S形变性曲线与二态模型之间的关系所作的详细分析，进一步强调变性过程表现为S型曲线，并非说明单结构域蛋白质的变性过程一定是二态过程。
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To develop standard WNV diagnostic tools, four monoclonal antibodies against WNV envelope protein domain III were produced. The isotype, the reactivity with denatured and native antigens, the affinity and specificity of antibodies have been characterized. Two MAbs (4B3 and 2F5) were used to establish an antigen capture-ELISA detection system.
为了建立有效的WNV检测方法，本研究制备了四株抗WNV囊膜蛋白第三结构域的单克隆抗体，鉴定了抗体的亚型、抗体和变性及非变性抗原结合的强度、抗原和抗体结合的亲和力常数，进行了免疫荧光分析、测定了抗原表位竞争、抗体的中和能力等，并检测了上述单抗与日本乙型脑炎病毒的交叉反应情况。
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For the geometrical reason,〓sphenylphosphinoethanein complexe(2)acts as an excellent〓elating ligand,the important feature of compound(3)and(4)is re-〓 to the single bridge dppe by which two tetrahedron 〓 or〓 linked together.
化合物（3）、（4）均涉及到叔膦dppe以单桥形式连结两个四面体〓，但化合物（3）为具有三种构象的流变性分子。
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The results show that single-screw extrusion system, the samples from the manufacturer complete, rheological properties better.
研究结果表明，采用单螺杆挤出成型制样，所得样品的塑化完全，流变性能较好。
- 更多网络解释与单变性的相关的网络解释 [注：此内容来源于网络，仅供参考]
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executory estate：待执行的财产权
cellular degeneratiaon 细胞衰退 细胞变性 | executory estate 待执行的财产权 | prosthodontics [复]n.[用作单][医]假牙修复学,假牙修制术
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monotrophic insect：狭食性昆虫
monotrophic 单变的,单食性的 | monotrophic insect 狭食性昆虫 | monotropic 单变,单变性的,单性
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monotropic：单变性的
monotropein 水晶兰苷 | monotropic 单变性的 | monotropicmonovariantunivariant 单变
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monotropic：单变 单变性的 单性
monotrophic insect 狭食性昆虫 | monotropic 单变,单变性的,单性 | monotropic function 单值函数