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Western blot analysis shows that the protein could be identified only by the antiserum to M. bovis but not the sera against.M, paratuberculosis, brucellosis, babesia and infectious bovine rhinotracheitis virus.
Western blot分析显示：该融合蛋白能与抗牛分枝杆菌阳性血清发生特异性反应，而与牛副结核病、牛布氏杆菌病、牛巴贝斯病和牛传染性鼻气管炎病阳性血清不反应。
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The result showed that the speciality of the reverse indirect hemagglutination diagnostic preparation is good.
该诊断液特异性强，仅与副猪嗜血杆菌5型阳性血清发生凝集反应。
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After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.
选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象，提取细菌基因组DNA，用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切，回收大小为1～5Kb的DNA片段，将其连接入原核表达载体pET-28a-c，最后转化宿主菌，结果成功地构建了基因表达文库，为后续的基因筛选工作奠定基础；另外，本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计，以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板，进行PCR扩增反应，结果表明成功地获得两个新基因：23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸)，并进一步做了不同物种之间的分子系统发育分析，构建了基于23S rRNA基因的邻接法系统发育树，阐明了副猪嗜血杆菌与其它菌种的分子进化关系。
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DNR：正定霉素
例如正定霉素(DNR)可以与接上了半乳糖末端残基的入血清白蛋白结合成肝靶向药物(DNR-Gal-HSA),用于肝癌的治疗,使70%的药物浓集在肝脏,降低药物的毒副反应.