- 更多网络例句与分枝杆菌相关的网络例句 [注:此内容来源于网络,仅供参考]
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It is the fact that three methionines distributed in the Hsp16.3 will begradually oxidated, in vitro, by hydrogen peroxide(H2O2). When the hsp16. 3 gene was cloned with pMV261 plasmid vector and transfected into Mycobacterium smegmatis, we found that the ability of anti-H2O2 will be decreased greatly among the strains of M. smeg. containing pMV261-hspl6. 3 while compared with the control strains of M. smeg. containing free plasmid vector. But we found that the ability of anti-H2O2 will be increased greatly among the strains of M. smeg. containing pMV261-38kDa while compared with the same control.
尽管Hsp16.3中有三个甲硫氨酸在体外能被过氧化氢(H_2O_2)逐一氧化,我们将Hsp16.3基因克隆到pMV261质粒上,并转染到耻垢分枝杆菌中发现,转染了pMV261-16.3的耻垢分枝杆菌抗过氧化氢的能力大大下降,而对照含pMV261-38kDa的耻垢分枝杆菌抗过氧化氢的能力却有明显的增强,据此,我们认为,Hsp16.3在细胞内可能不具有抗过氧化氢的能力。
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Pasteurization, which is often used for milk or waste milk, can be used to reduce bacteria levels and has been shown to be very effective at killing a variety of pathogenic bacteria including Salmonella, E. coli, Mycobacterium avium subspecies paratuberculosis, Mycobacterium californicum, Mycobacterium bovis, and Listeria monocytogenes.
巴氏灭菌常使用于牛奶或牛奶废料,它可以用来降低细菌水平,并已被证明能有效的杀死多种致病细菌,其中包括沙门氏菌、大肠杆菌、副结核分枝杆菌亚种、californicum分枝杆菌、牛分枝杆菌和李斯特菌。
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Respectively use chelex 100 resinate,boiling extraction,proteinase K digest/isopropanol precipitation,proteinase K digest/phenol/chloroformextra ction,alkaline lysis,guanidine thiocyanate lysis,guanidine hydrochloride lysis,spin column method to extract Mycobacterium tuberculosis DNA for multiplepolymerase chain eraction.
选择3份含结核分枝杆菌分别为4.325×103、6.857×104、5.356×105 Copies/ml的痰液,分别用Chelex 100树脂法、煮沸法、蛋白酶K消化异丙醇沉淀法、蛋白酶K消化酚/氯仿抽提法、碱裂解法、异硫氰酸胍裂解法、盐酸胍裂解法、离心柱法抽提结核分枝杆菌DNA作多重聚合酶链反应,显色法芯片检测结核分枝杆菌RFP和INH耐药基因。
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The results demonstrated that 22 of 50 were positive by PCR-ELISA method while only 7/50 and 18/50 by antiacid staining and culture respectively, and no positive result was found in 20 sputum specimens without Mycobacterium tuberculosis by the methods; Conclusion The pCR-ELISA method can objectively detect Mycobacterium tuberculosis from clinical specimens with high specificity and sensitivity.
对50份来自结核病人高度怀疑有结核分枝杆菌的标本检测表明,PCR-ELISA检出22份阳性,比抗酸染色法(7/50)、培养法(13/50)和PCR-电泳法(18/50)检出率高,而20份证实无结核分枝杆菌的标本,几种方法检查均为阴性。结论该法可敏感、特异和客观地检测临床标本中的结核分枝杆菌。
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In order to investigate new methods to the detection of Mycobacterium tuberculosis and diagnostic value of FQ-PCR in the detection of DNA in Mycobacterium tuberculosis, FQ-PCR was used to detect DNA of Mycobacterium tuberculosis in body fluid from 68 patients with tuberculosis and 68 patients without tuberculosis and it was also compared with the two methods of smear acid-fast stainning and antituberculotic antibody.
为探讨结核分枝杆菌检测的新途径及其FQ-PCR技术在结核分枝杆菌DNA诊断中的实用价值,运用FQ-PCR技术对68例结核病患者和68例非结核病患者体液进行结核分枝杆菌DNA测定,并与涂片抗酸染色及ELISA—抗结核抗体法进行比较。
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In this study we build a Mycobacterium marinum random mutant libratory with MycoMarT7 transposon phage, found two cell wall virulence genes with colony morphology and cording structure strategy and build up virulence detection system in cell and animal model. We concluded that PDIM is critical for the virulence of Mycobacterium marinum.
本课题利用MycoMarT7噬菌体建立了海分枝杆菌随机突变库,以海分枝杆菌为模型筛选出两个分枝杆菌细胞壁毒力相关基因,并建立了海分枝杆菌的毒力检测系统,发现PDIM在海分枝杆菌的致病性中起到重要重要的作用。
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The determination of differential proteins between Mycobacterium tuberculosis and nontuberculosis mycobaeteria may he helpful to differentiation of mycobacteria.
结核分枝杆菌和非结核分枝杆菌差异蛋白的发现,可能有助于分枝杆菌的鉴别。
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Results: Cultures for Mycobacterium were positive in a total of 2657 people, among them 1848 strains (69.55%) were Mycobacterium tuberculosis, and 809 strains (30.45%) were Nontuberculous Mycobacterium. Among the 1848 strains of Mycobacterium tuberculosis, 337 strains (18.24%) were drug resistant, and 75 strains (4.06%) of them were resistant to both Isoniazid and Rifampin which made them multi-drug resistant. Among the 548 strains of Nontuberculous Mycobacterium, 453 strains (82.66%) were drug resistant. There were a total of 261 rapidly growing mycobacteria, and 242 (92.72%) of them were drug resistant.
结果:分枝杆菌培养阳性共2657菌株/人,其中1848株(69.55%)为结核分枝杆菌,809株(30.45%)为非典型结核分枝杆菌感染。1848株结核分枝杆菌中,337株(18.24%)有抗药性问题,其中75株(4.06%)同时对Isoniazid和Rifampin有抗药性为多重性抗药菌。548株非典型结核分枝杆菌中453株(82.66%)有抗药性问题;快速生长菌群共261株,其中242株(92.72%)有抗药性问题。
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ABSTRACT:Nine proteins encoded by Mycobacterium tuberculosis RD1 region are important protective antigens that become absent in long passaging of Mycobacterium tuberculosis. They only exist in pathogenic Mycobacteria and are absent in Bacille Calmette-Guerin and environmental Mycobacteria. With good immunogenicities, they may play an important role in the diagnosis and prevention of Mycobacterium tuberculosis.
RD1区编码的9个蛋白,是结核分枝杆菌在长期传代过程中丢失的重要保护性抗原,仅存在于致病性分枝杆菌中,在卡介苗及环境分枝杆菌中缺失;其有较强的免疫原性,在结核病的诊断中发挥重要作用。
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The fusion DNA fragments of ag85b-mpb64 and ag85b-mpb64-esat-6 were obtained by PCR andSOE technique. Various DNA vaccines were constructed with the pcDNA3.1: fusion of two genes, and of three genes, bivalent combinations and trivalent combinations(pCA+pCM+pCE6). BALB/c mice were vaccinated with this DNA vaccines.The mice injected withBCG were positive control and the mice injected with pCDNA3.1 and PBS were negative control.The mice were immunized 3 times with 2-wk intervals. The animals in group BCG were only inoculatedsubcutaneously with 1×10~6 CFU BCG at initial vaccination. The serum IgG titers and IgG isotype weredetermined using iELISA coated with M. bovis PPD and rMAE protein expressed and depurated inprokaryotic expression system every week.
同样,利用PCR和SOE技术,获得牛分枝杆菌mpb64-ag85b和mpb64-ag85b-esat-6融合基因,以pCDNA3.1为载体构建了牛分枝杆菌多价组合和多基因融合DNA疫苗:二基因融合(pCDNA3.1-MPB64-Ag85B,简称pCMA)和三基因融合(pCDNA3.1-MPB64-Ag85B-ESAT-6,简称pCMAE)DNA疫苗;二价组合和三价组合(pCA+pCM+pCE6)DNA疫苗,免疫BALB/c小鼠,以牛分枝杆菌BCG免疫组为阳性对照,以pCDNA3.1及PBS免疫组为阴性对照,共免疫3次,每次间隔2周,BCG组仅初免时皮下免疫1次。1免后每周,以原核表达纯化的重组MPB64-Ag85B-ESAT-6蛋白和牛分枝杆菌PPD为包被抗原,以间接ELISA方法检测血清IgG水平及lgG亚类。
- 更多网络解释与分枝杆菌相关的网络解释 [注:此内容来源于网络,仅供参考]
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mau Mycobacterium aurum:金色分枝杆菌
1284 mas Mycobacterium asiaticum/亚洲分枝杆菌 | 1285 mau Mycobacterium aurum/金色分枝杆菌 | 1286 maa Mycobacterium austroafricanum/南非分枝杆菌
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Mycobacterium:分枝杆菌属
分枝杆菌属(Mycobacterium)是一类细长略弯曲的微生物,有时有分枝或出现丝状体.目前在分类学上已将分枝杆菌属归纳于放线菌中.对人致病的放线菌可分含和不含分枝菌酸两类.分枝杆菌属于含分枝菌酸类.形态与染色 结核分枝杆菌为细长略带弯曲的杆菌,
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Mycobacterium tuberculosis:分枝杆菌
引起结核病的分枝杆菌主要有三种,结核分枝杆菌(Mycobacterium tuberculosis)亦称人型结核菌,主要感染人、类人猿、猴、狗、牛等,也感染猪,但对猪病变一般认为仅局限于肠膜和颈部淋巴结,但现在已发现在猪肺部出现干酪样结核病;牛分枝杆菌(M.bovis),
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mycobacterium leprea:麻风(分枝)杆菌
mycobacteria 分枝杆菌 | mycobacterium leprea 麻風(分枝)杆菌 | mycobacterium trberculosis 結核(分枝)杆菌
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mycobacterium trberculosis:结核(分枝)杆菌
mycobacterium leprea 麻風(分枝)杆菌 | mycobacterium trberculosis 結核(分枝)杆菌 | mycobactin 分枝杆菌素
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mpa Mycobacterium paratuberculosis:鸟分枝杆菌
1326 mpf Mycobacterium parafortuitum/副偶发分枝杆菌 | 1327 mpa Mycobacterium paratuberculosis/鸟分枝杆菌 | 1328 mpg Mycobacterium peregrinum/外来分枝杆菌
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mpa Mycobacterium avium ss. Paratuberculosis:鸟分枝杆菌副结核亚种
1288 mac Mycobacterium avium complex/鸟分枝杆菌复合... | 1289 mpa Mycobacterium avium ss. Paratuberculosis/鸟分枝杆菌副结核亚种 | 1290 mai Mycobacterium avium-intracellulare complex/鸟-胞内分枝杆菌复合...
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mycobacteria:分枝杆菌/分枝细菌/分支杆菌
mycobacillin 分支杆菌素 | mycobacteria 分枝杆菌 分枝细菌 分支杆菌 | mycobacterialadjuvants 分枝杆菌佐剂
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Nontuberculosis mycobacteria:非结核分枝杆菌
非结核分枝杆菌(nontuberculosis mycobacteria)是指结核分枝杆菌,牛分枝杆菌与麻风分枝杆菌以外的分枝杆菌.原称为非典型分枝杆菌(atypical mycobacteria),其特性有别于结核分枝杆菌,如对酸,碱比较敏感;对常用的抗结核菌药物较耐受;生长温度不如结核分枝杆菌严格;
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atypical mycobacteria:非典型分枝杆菌
原称为非典型分枝杆菌(atypical mycobacteria),其特性有别于结核分枝杆菌,如对酸、碱比较敏感;对常用的抗结核菌药物较耐受;生长温度不如结核分枝杆菌严格;多存在于环境中;为条件致病菌.