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Extracts from fugu entrails had the biological function of agglutinating cells.
从河豚鱼内脏分离得到的一种具有凝集红细胞生物功能的活性物质。
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We used Aeromonas hydrophila which we separated from the diseased Paralichthys olivaceus to made a inactivated whole cell bacterin .We injected this vaccine in the mice and the Paralichthys olivaceus .Both the mice and the Paralichthys olivaceus gained a good protection of 80% and 70%.The fish with dipping immersion gained a good protection of 85%,and the incidence of disease was 6%.We detected the antibody level in the antiserum by agglutination reaction and indirect ELISA which seemed to be immunogenic crucially.We anatomized the mice then we found that all the purtenance of the immunzied mice are healty.All of this proved that the vaccine is effective.
本实验将所分离到的嗜水气单胞菌用终浓度为0.2%~0.5%的甲醛对菌体进行灭活,制备了灭活疫苗,并优化灭活疫苗的灭活条件;将制备的灭活疫苗分别注射小鼠和牙鲆,均取得了较为理想的效果,其中小鼠的免疫保护率达到80%,牙鲆的免疫保护率达到70%;采用渗透压差法大规模浸泡免疫牙鲆也取得了很好的效果,浸泡免疫过的牙鲆发病率仅为6%,攻毒后免疫保护率为85%;通过应用间接ELISA方法检测受免动物的抗血清,并将牙鲆的抗血清注射新西兰白兔,从而获得兔抗鱼血清,更便于检测,结果都表明动物体内产生了较高的抗体水平;剖检攻毒后的动物,发现免疫过的动物内脏健康无异样,表明该疫苗安全有效,从而为防治牙鲆腹水症开辟了一条新途径,也为进一步制备其基因工程疫苗奠定了基础。
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From its the depart in splanchnic organization arrives bacterium of 2 individual plant, pass bacteriological method appraisal, one individual plant is deputy pig bloodsucking bacili, another individual plant is a pig streptococcic; will send check sample book have an inoculation to marc-145 cell, can observe cellular pathological changes, the pcr of virus fluid classics of results detects, for positive of blue ear ill viru
从其内脏组织中分离到2株细菌,通过细菌学方法鉴定,一株为副猪嗜血杆菌,另一株为猪链球菌;将送检样本接种到marc-145细胞,能观察到细胞病变,收获的病毒液经pcr检测,为蓝耳病病毒阳性;将送检的样本接种spf鸡胚,收获的鸡胚尿囊液有血凝性,血凝试验和血凝抑制试验鉴定为猪副黏病毒。
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Achatina fulica:非洲大蜗牛
利用硅胶柱层析法和薄层层析法(TLE)分离乙酸乙脂浸泡非洲大蜗牛(Achatina fulica)得到的粗提物,对各纯化物的抗菌活性进行测定,并用气相色谱-质谱法技术分析抗菌物质的主要化学成分.结果表明其头足部和内脏团的主要抗菌物质成分不同,
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Septated unicollar uterus:单颈有隔子宫(畸形)
Separation of viscus 内脏分离 | Septated unicollar uterus 单颈有隔子宫(畸形) | Septic dysentery 脓毒性痢疾
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splanchnodiastasis:内脏分离 内脏移位
splanchnoderm 脏层 胚脏壁 | splanchnodiastasis 内脏分离 内脏移位 | splanchnodynia 内脏痛