- 更多网络例句与免疫素相关的网络例句 [注:此内容来源于网络,仅供参考]
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The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.
本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因,并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列,通过对克隆的降钙素序列的比较研究,结果显示同一科的鱼降钙素序列保守性较高,同时,根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究,结果显示在分类学上,分类地位较远的鱼,其降钙素相似性较低,分类地位越接近的鱼,其降钙素相似性越高;我们利用谷胱甘肽S-转移酶(Glutathions S-transferase,GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究,应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索,并将纯化的融合蛋白作为抗原,获得了高效价的兔抗鲑鱼降钙素免疫血清;生物活性研究表明,大肠杆菌表达的融合蛋白具有显著的降血钙作用。
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Results ①Compared to sham group, ISH and IHC of rat hypothalamic CRHshow positive cells numbers increase, positive areas enlarge and average gray scaledecreases, which reveals the expression of hypothalamic CRH neurons enhances afterPX;②The enhancement of ISH and IHC of hypothalamic CRH in PX rats aftermelatonin supplementation dose-dependently recovers, but it can't return to be normaleither in high dose group or in low dose group and either at 4th week or at 8th week;③IHC of CRH in median eminence of neurohypophysis shows positive areas enlargeand average gray scale decreases, which reveals the releasing of CRH fromhypothalamic CRH neurons increases;④ELISA of CRH in serum shows thedifferences of CRH concentrations among all groups are not significant, which indicates PX and melatonin supplementation have no significant effect on CRH levels.
结果: ①松果体摘除后大鼠下丘脑室旁核CRH 原位杂交和免疫组化的结果与假手术对照组相比均显示阳性细胞数增多、阳性面积增加、平均灰度降低,提示松果体摘除后下丘脑CRH 神经元的表达增强;②松果体摘除大鼠补充褪黑素后,下丘脑CRH 原位杂交和免疫组化染色增强呈剂量依赖性恢复,不过不论是低剂量组还是高剂量组,也不论是术后4 周还是术后8周,均不能恢复至正常;③松果体摘除后神经垂体正中隆起CRH 免疫组化结果显示阳性面积增加、平均灰度降低,提示下丘脑CRH 神经元释放CRH 增加;④ELISA 法检测血清CRH结果示各组血清CRH 浓度差异没有显著性,表明松果体摘除及补充褪黑素对血清CRH 水平没有显著影响。
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Results ①Compared to sham group, ISH and IHC of rat hypothalamic CRHshow positive cells numbers increase, positive areas enlarge and average gray scaledecreases, which reveals the expression of hypothalamic CRH neurons enhances afterPX;②The enhancement of ISH and IHC of hypothalamic CRH in PX rats aftermelatonin supplementation dose-dependently recovers, but it can't return to be normaleither in high dose group or in low dose group and either at 4th week or at 8th week;③IHC of CRH in median eminence of neurohypophysis shows positive areas enlargeand average gray scale decreases, which reveals the releasing of CRH fromhypothalamic CRH neurons increases;④ELISA of CRH in serum shows thedifferences of CRH concentrations among all groups are not significant, whichindicates PX and melatonin supplementation have no significant effect on CRH levels.
结果: ①松果体摘除后大鼠下丘脑室旁核CRH 原位杂交和免疫组化的结果与假手术对照组相比均显示阳性细胞数增多、阳性面积增加、平均灰度降低,提示松果体摘除后下丘脑CRH 神经元的表达增强;②松果体摘除大鼠补充褪黑素后,下丘脑CRH 原位杂交和免疫组化染色增强呈剂量依赖性恢复,不过不论是低剂量组还是高剂量组,也不论是术后4 周还是术后8周,均不能恢复至正常;③松果体摘除后神经垂体正中隆起CRH 免疫组化结果显示阳性面积增加、平均灰度降低,提示下丘脑CRH 神经元释放CRH 增加;④ELISA 法检测血清CRH结果示各组血清CRH 浓度差异没有显著性,表明松果体摘除及补充褪黑素对血清CRH 水平没有显著影响。
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The result indicated that the percentage of lymphocyte were decreased, the percentage of neutrophile granulocytes were magnified significantly with the lighting time increasing, which was due to the level of the melatonin inhibited in the long time lighting; and to supply different exogenous dose, induce the percentage of lymphocyte were magnifying, the percentage of neutrophile granulocytes were decreased. And the statistical analysis showed there was significant difference.
结果表明:在给予不同光照时间的条件下,随着光照时间的延长,褪黑素的分泌水平受到抑制,导致具有特异免疫功能的淋巴细胞百分率下降,具有非特异免疫功能的中性粒细胞百分率上升,经统计分析表明不同组间的测定结果均存在显着差异;采用24h全天光照,用以模拟机体切除松果腺的情况,再给予一定剂量外源性褪黑素,导致机体内具有特异免疫功能的淋巴细胞百分率上升,具有非特异免疫功能的中性粒细胞百分率下降,经统计分析表明不同组间的测定结果均存在显著差异,并且高剂量褪黑素对机体的影响更为明显。
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Objective To study the morphological and functional effect of pinealectomy on CRH neurons in paraventricular nucleus of rat hypothalamus as well as thefunction of melatonin supplementation, enrich the research of the effect of pinealbody and melatonin on hypothalamic-pituitary-adrenal axis,provide theoreticalevidence of the effect of pineal body and melatonin on immune function andimmune-associated disease such as tumor and autoimmune disease.
目的: 研究松果体及其褪黑素对大鼠下丘脑室旁核CRH 神经元形态和功能的影响以及补充褪黑素的作用,丰富松果体及褪黑素对下丘脑——垂体——肾上腺轴影响的形态及功能方面的研究,为进一步揭示松果体及褪黑素对免疫功能以及免疫相关性疾病如肿瘤、自身免疫性疾病等的影响机制提供理论依据。
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Through active immunization of Inhibin antige n from porcine seminal plasma and super ovulation of allochthonous purified FSH, the effect of IB active immunization on cow was studied and its mechanism was al so further ascertained by examination of relative hormone concentrations in seru m at different physiological periold.12 Hostein cows and 12 Xiza young cows of 3 -5 years old (postpartum 50 d and above),were selected and divided into the exp e rimental group and control group randomly according to the estrum order.
张志胜 ,田树军,王志刚,桑润滋用猪精液抑制素抗原主动免疫供体母牛,结合外源纯化FSH超排处理,观察了抑制素主动免疫对牛超排效果的影响,并通过检测不同生理时期血液中相关生殖激素的水平,探讨了抑制素免疫影响牛超排效果的机制。
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In order to study the effects of avilamycin and narasin on the non-specific immunity of Cyprinus carpio, avilamycin and narasin were mixed with feed in different concentrations respectively.
本研究以鲤为试验对象,在饲料中分别添加不同浓度的阿维拉霉素和甲基盐霉素,研究阿维拉霉素和甲基盐霉素对鲤非特异性免疫机能和免疫应答能力的影响。
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The results indicated that avilamycin has significant effects on the non-specific immunity of Cyprinus carpio.
对鲤投喂适宜浓度(4mg/kg—8mg/kg)的阿维拉霉素,短期内有一定的免疫促进作用,投喂较长时间后会发生免疫抑制;对鲤使用高浓度的阿维拉霉素(≥16mg/kg)时,会迅速发生免疫抑制。
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Polyclonal antibodies for Ochratoxin A were generated from rabbits after the animals had been immunized with either OTA -γ- globulin or OTA - keyhole limpet hemocyanin. A competitive indirect enzyme - linked immunosorbent assay and a competitive direct ELISA were used for the characterization of the antibodies and for analysis of OTA in coffee, corn, barley and bean samples.
本实验利用OTA -γ- globulin和OTA - KLH两种不同的OTA接合物为抗原来免疫兔子,进而收集免疫血清以获得抗OTA 的多株抗体,接著利用此一抗体来建立非直接型竞争酵素连结免疫吸附分析法和直接竞争型酵素连结免疫吸附分析法,并用cdELISA的方法加以侦测咖啡及玉米类、麦类、豆类样品中赭麴毒素A 的含量。
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objective the aim of this study is to investigate the expression and the distribution of the nerve growth factor during the period of neural tube development of human embryo.method early development of neural tube was studied in human embryos about 35 gestational days by using immunocytochemical abc technique.result there were ngf immuno-positive substances in the cytoplasm and nuclei of neuroepithelial cells in the ventricular zones of neural tube.in the intermediate zone of neural tube,ngf immunoreactivity was detected in the nuclei of some neurons,or the processes of other neurons which contained no ngf-immunoreactive substances in their nuclei;the expression pattern of ngf in the marginal zone of neural tube was similar to that of the intermediate zone.the density of ngf-immunorecative particles was higher on the rostrum side of neural tube than on the caudal side.the ngf immuno-positive cells were also observed among the somites of embryo under the neural tube.conclusion these results suggest that ngf was an important signal molecule to induce neural tube differentiation,and that ngf may play a significant role in regulation of the biological function of neurons in developing neural tube.
目的 研究捷安肽素的抗真菌作用机理。方法采用形态学方法和同位素标记法。显微形态观察经捷安肽素处理后的供试真菌的形态学变化。进一步采用14c同位素标记的特异底物&尿苷二磷酸-(14c)-葡萄糖&示踪,研究捷安肽素对真菌(1,3)-β-d-葡聚糖合成酶活性反应的影响。结果研究神经生长因子在早期人胚神经管发育过程中的定位表达。方法采用免疫细胞化学 abc法染色,研究35天人胚的发育情况。结果在人胚神经管的室管带中,神经元的细胞质和细胞核ngf免疫反应阳性;在中间带,一部分神经元的细胞核ngf免疫反应阳性,另外一部分神经元的细胞核ngf免疫反应阴性,而其突起ngf免疫反应阳性;在边缘带ngf的表达与中间带相似。在神经管的头侧ngf阳性反应较强,神经管的尾侧ngf阳性反应较弱。结论 ngf在人胚神经管免疫反应阳性,表明ngf可能是诱导神经管分化发育的重要信号分子,提示ngf可能在人胚神经管的发育中具有十分重要的作用。神经生长因子;人胚;神经管;发育
- 更多网络解释与免疫素相关的网络解释 [注:此内容来源于网络,仅供参考]
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immune hemagglutinin:免疫血凝素
immune hemagglutination 免疫性血细胞凝集 | immune hemagglutinin 免疫血凝素 | immune hemolysin 免疫溶血素
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immune hemolysin:免疫溶血素
免疫功能 immune function | 免疫溶血素 immune hemolysin | 免疫干扰素 immune interferon
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immune lactoglobulin:免疫乳球蛋白
immune isohemagglutinin 免疫同族血凝素 | immune lactoglobulin 免疫乳球蛋白 | immune lysin 免疫溶素
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Immune precipitin:免疫沈淀素
免疫凝集素 Immune agglutinin | 免疫沈淀素 Immune precipitin | 免疫质 Immune substance
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bacteriotropin:亲菌素 趋菌素 免疫调理素
bacteriotropicserum 亲菌性血清 | bacteriotropin 亲菌素 趋菌素 免疫调理素 | bacterioviridin 绿菌属叶绿素
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gastrin:胃泌素
(MBP) 酶联免疫/酶免法(ELISA) 96T狗髓鞘碱性蛋白(dog MBP) 酶联免疫/酶免法(ELISA) 96T人 同型半胱氨酸(HCY) 酶联免疫/酶免法(ELISA) 96T人 同型半胱氨酸(HCY) 酶联免疫/酶免法(ELISA) 96T人 胃泌素(Gastrin) 酶联免疫/酶免法
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opsonic index:调理指数;助噬素指数
助噬素免疫;调理素免疫 opsonic immune | 调理指数;助噬素指数 opsonic index | 调理素;助噬素 opsonin
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opsonic immune:助噬素免疫;调理素免疫
鸦片 opium | 助噬素免疫;调理素免疫 opsonic immune | 调理指数;助噬素指数 opsonic index
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immunise:使免疫/赋予免疫性
immunifaction /免疫法/ | immunise /使免疫/赋予免疫性/ | immunisin /免疫素/
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enzyme immunoassay:酵素免疫分析法
297 酵素 enzyme | 298 酵素免疫分析法 enzyme immunoassay | 299 酵素连结免疫电渍技术 enzyme-linked immunoelectrotransfer blot technique