体素培养

词组短语

tissue culture

与体素培养相关的网络例句 [注：此内容来源于网络，仅供参考]

Furthermore, from the result of inoculation with fungi in vivo, some animalcula stimulate the formation and accumulation of dragon's blood. 3. According to the results of zymolysis culture of some animalcula, there are a small quantity of loureirins in the zymolysis products, contents of loureirin A range from 0.090 to 0.439mg/g, loureirin B from 0.012 to 0.133mg/g.

从某些代谢产物呈红色或菌体本身呈红色的菌的液体发酵培养结果来看，这些菌的发酵产物中有龙血素成分存在，但量极少，龙血素A、B的含量分别在0.090-0.439mg/g，0.012-0.133mg/g范围之类，并且其含量随菌的培养时间的增长有增加的趋势。

Result Based on directly regenerated plant of in vitro plant of Xinjiang cotton, the cotton epicotyl treated by pre-culture for 3-5 d was immersed in bacteria for 15 min and co-cultured for 2 d. Cephalothin at 100 mg/L was used to inhibit bacteria after 50 mg/L kan selection. And the combination of cytokinin with relatively high content and auxin with low content was used in its directly budding culture to obtain resistant seedlings.

结果］在新疆棉花离体培养植株直接再生植株基础上，经3~5d预培养处理过的棉花上胚轴，在浸菌15 min共培养2 d后，经50 mg/L kan筛选，用1000 mg/L头孢霉素抑菌，并用相对高含量细胞分裂素和低含量生长素的组合对其进行直接出芽培养，从而获得抗性小苗。

ResultBased on directly regenerated plant of in vitro plant of Xinjiang cotton,the cotton epicotyl treated by pre-culture for 3-5d was immersed in bacteria for 15min and co-cultured for 2d.Cephalothin at 1000mg/L was used to inhibit bacteria after 50mg/L kanselection.And the combination of cytokinin with relatively high content and auxin with lowcontent was used in its directly budding culture to obtain resistant seedlings.

结果在新疆棉花离体培养植株直接再生植株基础上，经3～5d预培养处理过的棉花上胚轴，在浸菌15min共培养2d后，经50mg/Lkan筛选，用1000mg/L头孢霉素抑菌，并用相对高含量细胞分裂素和低含量生长素的组合对其进行直接出芽培养，从而获得抗性小苗。

The results showed that the Saccharopolyspora spinosa SP06081 protoplast yield was the highest under these conditions: the collected mycelium from SP06081 grown in Tryptic Soy Broth medium with 0.2% glycin for 48 h was treated by 0.1 mg/mL lysozyme at 28oC for 20 min, then plated on the R2YE medium with sucrose as osmotic stabilizer, the number of regeneration protoplast was up to 108/mL. The protoplast-regenerated strains exhibited changes in morphology and antibiotic production, 29.3% protoplast-regenerated strains was characterized by loose mycelium and abundant broken branches as did their parent. Among them, 58.2% strains presented the trend to positive variation in spinosad yield, with the highest spinosad yield of up to 582.0 mg/L, 85.6% higher than that of their parent.

结果表明：菌体在添加0.2%的甘氨酸的TSB培养基中培养48 h收集， 0.1 mg/mL 溶菌酶， 28oC作用20 min制备原生质体，将原生质体涂布于以蔗糖为渗透压稳定剂的R2YE培养基中，原生质体再生数目最多，达108个/mL以上；原生质体再生菌株在形态和抗生素产量上产生分化， 29.3%的再生菌株形态上保持与亲本菌株一致，具有菌丝松散，断裂分枝多的特点，其中53.2%的再生菌株多杀菌素产量变异向正方向移动，最高产量达到582.0 mg/L，比亲本菌株提高85.6%。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Results showed that, pyrolin had obvious action on two species of fungi, and the antifungal effect was positively correlative with concentration. The EC50 was 0.4076 mg/mL and 0.4626 mg/mL respectively after cultured 3 days. The main antifungal period was the fast growth period of fungi, and lead to it lag behind. Observation under SEM revealed that pyrolin caused a series of marked morphological and structural alterations of hyphal. These changes included mutual adhesion of mycelium, the hyphal was irregular in diameter, local denting, some of mycelium was broken, disruption of cell wall, outbreaking of inner cytoplast, and the empty cavity appeared. As a result, pyrolin restrained the growth of these fungi.

结果表明，鹿蹄草素对上述两种真菌均具有明显的抑制作用，抑菌效果与浓度呈正相关，培养3d时的EC50分别为0.4076、0.4626 mg/mL；抑菌时期主要是在真菌的快速生长期，使其快速生长期滞后；扫描电镜显示，鹿蹄草素作用下菌体形态发生异常变化，表现为菌丝体之间相互粘连，菌丝粗细不均匀，细胞壁破裂，局部凹陷，部分菌丝断裂，大量内容物外溢，出现空腔等现象，从而发挥其抑菌作用。

The uropoiesis genital tract mycoplasma infects the Uu masculine gender rate to be highest, the feminine masculine gender rate is highest; The mycoplasma medicine sensitive first choice medicine is the force mildew element, junction Sha Meisu, the content mildew element; Must use the mycoplasma appraisal agaragar culture medium to make the mycoplasma proof raise.

泌尿生殖道支原体感染Uu阳性率最高，女性阳性率最高；支原体药敏首选药物是强力霉素、交沙霉素、美满霉素；必须使用支原体鉴定琼脂培养基做支原体确证培养。

Methods From June 2005 to July 2006,60 patients of Juancheng Family Planning Hospital with positive results for Uu or Mh culture of uterocervical secretion were randomized into two groups.

方法将宫颈分泌物支原体培养阳性者随机分成两组，对照组为单纯应用阿奇霉素治疗组；观察组为安尔碘液联合阿奇霉素治疗组；两组分别于治疗开始的第10天进行宫颈拭子支原体培养，阳性者间隔两周再次复查，根据培养结果，结合症状体征判断疗效。

The present study is involved in the expression of vitronectin , a ligand of integrin, on human spermatozoa and its role in fertilization; the expression of integrin α〓、α〓、β〓 sbunits on human and mouse oocytes and their relationship with maturation of oocytes; the reorganization of cytoskeletal protein, actin, of mouse oocytes mediated with RGD peptide binding with integrins; the roles and mechanisms of integrins in activation of mouse oocytes as trasmembrane signaling receptors, using the methos of cell culture, immunohistochemistry, flow cytometer, laser confocal microscope.

本课题采用细胞培养、免疫组化、流式细胞术、激光共聚焦显微技术进行以下四个方面的研究：整合素配体玻连蛋白在人精子的表达及其与精子功能状态及受精的关系：整合素〓在人、小鼠卵的表达及其与卵的成熟程度的关系：整合素介导小鼠卵内actin骨架蛋白的重组；整合素介导卵激活的早、晚期事件，包括卵细胞内钙离子浓度的变化及孤雌发育胚的形成，并探讨其机制。

NJWGY3665 bacterial strain is inoculated in a glucose nutrient culture medium to carry out the slant culture, the culture temperature is 28 to 37 DEG C and the culture time is 2 to 6 days;(2) seed culture: a spore which is cultured on a slant is produced into a single-spore suspension by using sterile water, which is also inoculated in a seed culture medium for culture, the temperature is 28 to 32 DEG C, the rotational speed is 200rpm and the culture lasts for 2 to 6 days;(3) fermentation culture: the seed liquid is inoculated in a fermentation culture medium for culture, the temperature is controlled at 28 to 37 DEG C, the pH is controlled at 6.0 to 9.0, the rotational speed is 180 to 220rpm, the fermentation lasts for 5 to 9 days, so as to obtain the Streptomyces sp., methanol or ethanol is used for the extraction and separation of mycelium through the method of centrifugal separation, then the resin method is adopted for carrying out refining, so as to obtain the peptide antibiotics enramycin.

NJWGY3665菌种接到葡萄糖营养培养基中，进行斜面培养，培养温度28-37℃，培养时间2-6天；(2)种子培养：用无菌水将斜面上培养的孢子制成单孢子悬液，并接种到种子培养基中培养，温度为28～32℃，转速为200rpm，培养2-6天；(3)发酵培养：将种子液接种到发酵培养基中培养，温度控制在28-37℃，pH控制在6.0-9.0，转速180-220rpm，发酵5-9天，得到链霉菌，采用离心分离的方法将菌丝体用甲醇或乙醇进行提取分离，再采用树脂方法进行精制，获得多肽类抗生素安来霉素。

与体素培养相关的网络解释 [注：此内容来源于网络，仅供参考]

cardinal：红衣主教

并在离体培养过程中对试管苗的生根、移栽基质及影响试管苗移栽成......该文通过对粉团蔷薇和多花蔷薇无刺品系No.3、No.4为砧木,切花月季'红衣主教(Cardinal)'为接穗的嫁接植株生长量、内源玉米素核苷(ZR)和脱落酸(ABA)含量,

embryo culture：胚培养

自然界不可能交配的品种间之杂交,利用胚培养(embryo culture)或细胞融合方法已能办到(如白菜与甘蓝利用酵素,抗体等特异性高的反应,将这些生体成分固定於膜上的生物感测器,将成为电子工业发展目标之一.

KT：激动素

在冬芽的离体培养过程中,细胞分裂素是茎叶生长的必要条件,其中又以6-苄基腺嘌呤(BA)的生长促进作用最强,玉米素(ZT)次之,激动素(KT)最弱,生长素(IAA)与赤霉素(GA3)对培养冬芽茎叶的生长几乎无效,脱落酸(ABA)则起抑制作用.

alkaline phosphatase：碱性磷酸

胚干细胞继代培养,通常 用酵素如胶原蛋白(collagenase) 或胰蛋白(trypsin)胚干细胞未分化 态所表现的分子标志 鉴定,包括(a)碱性磷酸(Alkaline Phosphatase)胎小体的培育过程,直接将胚干细胞培养在基质细胞(stromal cell)上,

tet：四环素

前列腺颗粒 未见 滴虫 未见 念珠菌 未见 支原体衣原体化验结果如下: 沙眼衣原体抗原(沙眼) 阴性 支原体培养(UU/MH) 解腺腺原体 红霉素(ERY) 敏感 乙铣螺旋霉素(ACE) 耐药 交沙霉素(JOS) 敏感 四环素(TET) 敏感 强力霉素(

Zinnia elegans：百日草

在百日草(Zinnia elegans)的单细胞培养中,细胞分裂素和生长素的共同使用也促进导管成分的分化. 赤霉素和生长素之间的相互作用也能对木质部的分化产生积极作用. 无论是在离体还是在活体条件下,植物细胞分化的研究重点是维管组织的分化,

JOS：交沙霉素

请专家帮我分析下.我检查的是白带(普通细菌培养+药敏):检查结果为 支原体培养+鉴定+计数+药敏 检出解脲脲原体(大于等于)10的4次方 四环素(TET) 耐药 氧氟沙星(OFL) 耐药 红霉素(ERY) 耐药 强力霉素(DOX) 敏感 交沙霉素(JOS) 敏感 司帕沙星(