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Porcine was acted as the experimental animal. It was researched on problem of the origin of obtaining early embryonic cells, methods of separating blastomere cells, best parameter and order of ECNT embryo electric activation and system of ECNT embryo IVC, which was to find a scientific method of fitting ECNT and a procedure of fitting the lab producing embryos largely, optimize the procedure of ECNT and improve the ECNT embryo in vitro.
本实验以猪为实验动物,对猪胚胎细胞核移植供体的来源、卵裂球细胞分离方法、核移植重建胚的电激活最佳参数、核移植受体的激活时序以及核移植重建胚体外培养系统相关问题进行了研究,旨在探索一种适用于猪胚胎细胞核移植的科学方法,提高猪胚胎细胞核移植胚胎的体外发育率,优化胚胎细胞核移植程序。
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The three types of reconstructed embryos(reconstructed embryo of goat-rabbit, goat-bovine and goat-goat) were produced respectively by nuclear transfer using goat ear firbroblast cells as donors and rabbit, bovine and goat ooctyes as recipients, and the factors of cytoplast environments influencing cloned embryo development in vitro, cytoplast effect in nuclear transfer, the methods of transferring cloned embryo and the effects of pregnancy factor on implantation of cloned embryo were studied in this experiment, in order to supply basis for solving the difficulty of implantation of inter-species cloned embryo in recipient and improving the efficiency of cloning.
本试验以波尔山羊耳成纤维细胞为供体,分别以牛、羊、兔的卵母细胞为受体进行体细胞核移植,构建了山羊-兔、山羊-牛和山羊-山羊三种重构胚。研究了不同的胞质环境对重构胚体外发育的影响与核移植中的胞质效应,并对异种克隆胚胎采用了去透明带移植和配种后再移植的方法,探讨移植方法和妊娠因子对异种克隆胚胎在受体动物体内发育的影响,旨在为解决异种克隆胚移植不易着床发育的难题和为提高动物克隆效率提供理论依据。
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The results shown as follow: 1. The study on mice denuded oocytesin vitro maturation, in vitro fertilizationand in vitro development. This part of paper is to discuss whether the remove of granulose cells can affect the mice oocytes maturation rates, fertilization rates and development rates in vitro.
一、去除卵丘细胞的小鼠卵母细胞的体外成熟、体外受精初步研究本实验探讨了不同成分明确的成熟培养液对去除卵丘细胞的小鼠卵母细胞的体外成熟、体外受精和胚胎的体外发育的影响。
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Results coculture with endometrial epithelial cells could promote the formation of mouse morula (71.3% and 61.2%,p.05) and blastocyst (50.0% and 39.7%,p.05), and increase the blastocyst hatched rate (34.8% and 24.3%,p.05) and total cell number of blastocyst (44.1 and 35.6, p.05).conclusion coculture with endometrial epithelial cells can facilitate the development of early mouse embryo in vitro, which can optimize the condition of embryonic development in vitro.
结果:与无上皮细胞相比,共培养明显促进了小鼠桑椹胚率(71.3%和61.2%,p.05),囊胚率(50.0%和39.7%,p.05),囊胚孵化率(34.8%和24.3%,p.05)和胚胎总细胞数(44.1和35.6,p.05)。结论:子宫内膜上皮细胞能够促进小鼠胚胎的体外发育,对早期胚胎的体外发育环境具有优化作用。
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Based on the experience and methods summed up by other researchers, the technique of cell culture was employed to study bovine follicular oocytes maturation, fertilization and the development of earlier embryo in vitro. The objective of this research is to upbuild and perfect a set of high-and-new-tech systems to obtain high-yield and high-quality bovine embryo, to provide technical operational regulations and ways and means to embryo engineering in practical production, to accumulate data for theoretical research.
本研究在总结前人研究的基础上,应用体外培养技术对牛卵母细胞体外成熟、体外受精及早期胚胎体外发育作了进一步研究,旨在建立健全一套完整的体外生产高产优质牛胚胎的高新技术体系,为胚胎工程和生产实践提供技术操作规程和方法,为理论研究积累资料。
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To optimize the in vitro culture system of mouse pronuclear zygote and to investigate the effect of FBS, PVA, hLIF on mouse zygote development in vitro, we did four experiments separately.
筛选最佳的小鼠胚胎体外发育培养体系,并进一步探讨不同浓度的FBS、PVA、hLIF对其体外发育的影响,从而为小鼠卵母细胞核移植后重构胚的体外培养提供支持。
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The present study was conducted to investigate the effect of 6-DMAP on parthenogenetic activation rate, pronuclear formation and in vitro parthenote development of in vitro matured goat oocytes.
体外成熟的山羊卵泡卵母细胞用7%的乙醇和5μmol/L的离子霉素刺激处理后,分别置于含2 mmol/L 6-二甲基氨基嘌呤(6-DMAP)和不含6-DMAP的培养液中培养4 h,研究6-DMAP对体外成熟山羊卵泡卵母细胞孤雌激活率、原核形成以及孤雌胚体外发育潜能的影响。
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Tolerance threshold of 2-cell mouse embryos to ethanol was around 1.5% when these embryos were cultured in Whitten's medium with 0, 0.1%,0.5%,1.0%, 1.5%, 2.0%, 3.0%, 5.0% and 10.0% ethanol, respectively.
用含不同浓度乙醇的Whitten氏培养液对小鼠2细胞、4细胞、8细胞和桑椹期胚胎分别进行体外培养,研究了乙醇对小鼠不同发育时期胚胎体外发育的影响。
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Coculture with endometrial epithelial cells can facilitate the development of early mouse embryo in vitro, which can optimize the condition of embryonic development in vitro.
子宫内膜上皮细胞能够促进小鼠胚胎的体外发育,对早期胚胎的体外发育环境具有优化作用。
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Light microscope combined with stereological methods were used to observe and analyze renal tissue and corpuscles at different developmental stages in vivo and vitro.
结果 胚龄12天(E12)体外培养发育情况与在体无显著差异,其余各组随胚龄的增加,小鼠肾组织的体外发育呈下降趋势。
- 更多网络解释与体外发育相关的网络解释 [注:此内容来源于网络,仅供参考]
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ectogenesis:体外发育
ectoenzym 外酶 | ectogenesis 体外发育 | ectogenetic 体外发育的
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funiculus:胃绪
能入行配子生殖每个个体具有一个在体腔膜上形成的卵巢和发生在体腔膜形成的胃绪(funiculus)上的多个精巢受精卵在体内或体外发育海产种类有一似担轮幼虫的幼虫期通常以出芽法行无性生殖可产生休眠芽,
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in vitro culture:体外培养
体外培养(in vitro culture)就是将活体结构成分(如活体组织、活体细胞或者活体器官等)从体内或其寄生体内取出,放在类似于体内生存环境的体外环境中,让其生长发育的方法.
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in vitro fertilization IVF:离体受精,体外受精
in vitro developmental block 体外发育阻断 | in vitro fertilization IVF 离体受精,体外受精 | in vitro maturation IVM 体外成熟
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Bufo gargarizans:中华蟾蜍
1.体外受精体外发育 绝大多数的两栖动物营这种繁殖方式,我们经常见到的黑斑蛙Rananigromaculata)、中华蟾蜍(Bufo gargarizans)等都是以这种方式进行,这些动物一般将卵产于水中,受精卵在水中完成其胚胎发育.
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ectogenic:外生的
ectogenetic 体外发育的 | ectogenic 外生的 | ectogenous 外生的
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ectogenic:外生的; 体外发育的 (形)
ectoderm 外胚层, 外细胞层 (名) | ectogenic 外生的; 体外发育的 (形) | ectogenous 外生的; 体外生活的 (形)
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ectogenous:外生的; 体外生活的 (形)
ectogenic 外生的; 体外发育的 (形) | ectogenous 外生的; 体外生活的 (形) | ectohormone 外激素 (名)
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Ectoprocta:外肛動物
肛门开口于总担的外侧,故又称外肛动物(Ectoprocta). 缺乏肾管和循环系统. 苔藓动物为雌雄同体,能进行配子生殖. 每个个体具有一个在体腔膜上形成的卵巢和发生在体腔膜形成的胃绪(funiculus)上的多个精巢,受精卵在体内或体外发育.
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tubers in vitro:离体块茎
体外实验:in vitro modeling | 离体块茎:tubers in vitro | 体外发育:In Vitro Development