- 更多网络例句与二氢荧光素相关的网络例句 [注：此内容来源于网络，仅供参考]
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METHODS: The BEC were preincubated with crocetin (1, 0.1 and 0.01 μmol/L) for 12 h, then exposed to AGE (100 mg/L). The RAGE mRNA expression was detected by RT-PCR analysis, the intercellular adhesion molecule-1(ICAM-1) was measured by ELISA. The extracellular superoxide ion and thiobarbituric acid reactive substances were assessed with superoxide ion kit and colorimetric assay, respectively The intracellular I2O2 was also detected using the probe 2,7-dichlorofluorescein. The mitochondrial membrane potential and mitochondrial Succinate dehydrogenase were analyzed by the retention of rhodamine 123 (Rh123) and MTT.
不同浓度的西红花酸(1、0.1、0.01μmol/L)预孵BEC细胞12h后，用AGE(100mg/L)刺激细胞12h，RT-PCR法测定RAGEmRNA的表达水平；ELISA法测定细胞间黏附分子－(ICAM-1)的表达；试剂盒分别检测胞外超氧阴离子和硫代巴比妥酸反应产物浓度；同时，还用2，7-二氯荧光素测定了胞内H2O2的浓度，并用罗丹明123(Rh123)荧光法及MTT法分别检测细胞线粒体膜电位水平和其琥珀酸脱氢酶的活性。
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DCHF is a nonfluorescent compound that can beoxidized to 2', 7'-dichlorofluorescin, a fluorescent product, by ROS in cells,which can be monitored by fluorescent microscope or confocal laser scanningmicroscope.
DCHF进入细胞后被ROS夺取二氢后氧化成二氯荧光素，用荧光显微镜和激光共聚焦显微镜观察DCF的荧光强度，表示ROS生成量的多少。
- 更多网络解释与二氢荧光素相关的网络解释 [注：此内容来源于网络，仅供参考]
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fluorescin; dihydrofluorescein：二氢荧光黄
荧光素钠 fluorescein sodium | 二氢荧光黄 fluorescin; dihydrofluorescein | 氟醋酸;氟乙酸 fluoroacetic acid