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130 rats were randomly divided into five groups: 5F electroacupuncture treatment group, 50Hz electroacupuncture treatment group,100Hz electroacupuncture treatment group, the model group and the control group. 4mm defect was produced in the left sciatic nerve of each rat and silicon nerve guards were used to bridge the nerve defect not including the rats of the control group. After 10 weeks electroacupuncture treatment, gross observation, histological examination of the sciatic nerve were carried with method of HE stainning and S-100 immunhistochemical staining, Sevier-Munger stainnning. The histomorphometric examination of the triceps muscles were observed with measuring width muscle cell and the wet weight, and measuring the content of the heparin and the lactic aid of the triceps muscle with biochemistry methods.
实验方法：将130只Wistar大鼠随机分为五组：正常组、造模组、电针5Hz组、电针50Hz组、电针100Hz组，除正常组外，其余各组手术切取坐骨神经4mm后，做坐骨神经硅胶管桥接术，造成周围神经损伤模型，取患侧足三里、三阴交、环跳穴，术后第四天开始电针，每两天一次，每次10分钟，治疗10周后处死动物，取出硅胶管内新生的坐骨神经，纵切片，HE染色、S100免疫组化染色、Sevier-Munger银染，观察坐骨神经纤维再生情况，以健侧同段坐骨神经做对照；取患侧脖肠肌称取湿重，HE染色，观察腓肠肌肌细胞直径，生化测量腓肠肌肌糖元、乳酸。
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In this paper,the Lactate dehydrogenase, malate dehydrogenase,superoxide dis-mutase of five tissues of Carassius auratus gibelio; Cyprinus carpio Linnaeus; Esox americanus Gmelin; Leuciscus waleckii in Dalai Lake were studied by vertical polypropylene gel electrophoresis and the isozyme composion of four kinds of fishes were obtained. Then the analysis of the expressing specify of 3 kinds of isozyme in different tissues and loci were carried on and the genetic base of isozyme system was discussed.
本实验采用垂直板聚丙烯酰胺凝胶的电泳方法对达赉湖的野生鲤鱼、银鲫、狗鱼、东北雅罗鱼的五种组织脏器(肌肉、心脏、肝脏、脑、眼睛)的三种同工酶(苹果酸脱氢酶、乳酸脱氢酶、超氧化物岐化酶)进行了初步研究，获得了达赉湖四种鱼类物种的同工酶谱，在此基础上对同工酶在不同组织的表达差异和基因位点进行分析，探讨其同工酶系统的遗传基础。
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The Surface Enhanced Raman Scattering SERS e ffect from Calcium lactate CLon Ag mirror is studied in this paper.
利用银镜作为衬底，本文对乳酸钙的表面增强喇曼散射效应进行了研究。
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TTT：麝香草酚浊度试验
反射层一般由银介质以及其他化学物质组成;一、血清酶学检查:丙氨酸转氨酶(ALT)天门冬氨酸转氨酶(AST)碱性磷酸酶(ALP)乳酸脱氢酶(LDH) 二、肝功能检验:总胆红素(TBIL)直接胆红素(DBIL) 间接胆红素(IBIL) 麝香草酚浊度试验(TTT)
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silver lactate：乳酸银
柠檬酸氢银 silver hydrogen citrate | 乳酸银 silver lactate | 月桂酸银;十二酸银 silver laurate
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silver hydrogen citrate：柠檬酸氢银
柠檬酸氢银 silver hydrogen citrate | 乳酸银 silver lactate | 月桂酸银;十二酸银 silver laurate