两细胞的

基本解释 (translations)

bicellular

与两细胞的相关的网络例句 [注：此内容来源于网络，仅供参考]

First, to isolate the total RNA both in hypha and yeast phase of Malasesiza furfur, Then reverse them to cDNA and found a different cDNA library with Malasesiza furfur in hypha phase by Subtractive Hybridization; At last, clone and sequencing the gene fragment on differential expression of Malasesiza furfur in hypha phase.

首先提取糠秕马拉色菌两相细胞的总RNA；然后逆转录生成两相细胞cDNA，运用消减杂交技术获得菌丝相细胞差异表达的cDNA，并建立差异文库：最后对差异cDNA片段克隆测序。

They discovered that vaccinia produces two proteins right after it infects a cell.

他们发现，痘苗感染一个细胞之后立即产生两种蛋白质，两种蛋白质结合在一起在细胞表面形成一种复合物，这种复合物能够阻止其他的痘苗病毒微粒进入细胞（病毒再次侵入细胞的过程叫做"重叠感染"）。

Impact of PCL capsulation on the mesenchymal stem cell activity studied in vivo, one plate culture with complete culture medium for control group with and two encapsulated transplantation for experimental groups(complete culture medium or basic culture medium for cell suspended) installed,after package and transplantation,retrieving capsulated cells for cell count,MTT assay,CCK-8 assay Daily for 8days to determine cell activity,and analysis growth curve.

以完全培养液平皿法细胞培养为对照组，设2个实验组进行聚己内酯囊管化骨髓间充质干细胞移植细胞活性研究。两个实验组分别以完全培养液和基础培养液悬浮细胞后封装移植，囊管内细胞回收后以细胞计数、MTT法、CCK-8法测定细胞的活性，共8天，进行生长曲线分析。

Results: ApoD was observed positively expressed in type2 astrocytes and oligodendrocytes from O2A lineage, while it was not expressed in type1 astrocytes from T1A lineage. Accordingly, we can verify the phenomenon found in the study of T1A and T2A gene different expression profile that ApoD mRNA was highly expressed in T2A and lowly expressed in T1A. Conclusion: It can be concluded that lipid metabolism related genes such as ApoD play important roles in the generation and differentiation of O2A lineage, which closely correlate with internal mechanism of lipid metabolism in the brain and myelogenesis.［Key words］astrocyte(T1A, T2A); oligodendrocyte; O2A lineage; apolipoproteinApoD

我们已采用基因芯片技术在mRNA水平研究两型星形胶质细胞的基因表达谱差异，发现ApoD在2型星形胶质细胞中差异高表达，故本文拟用激光共聚焦双重免疫荧光标记技术进一步在蛋白质水平观察ApoD在体外分化成熟的两型星形胶质细胞以及少突胶质细胞中的表达情况，以期为我们深入研究ApoD等脂代谢相关基因在脑内脂质代谢和神经髓鞘发育机制中所起的作用提供有用的实验依据，依此探讨胶质细胞发生和分化的信号机制。

objective:to analyze the difference between mammary duct ectasiaand plasma cell mammitis.methods:the data of 24 cases of mde and 28 cases of pcm,including clinical manifestations,distinguished diagnoses,surgical therapy,pathological results,were analyzed.results:the clinical manifestations of mde are nipple discharge and breast mass,which should be distinguished with breast mass and early stage of breast cancer.the treatment is local resection.major pathological changes are duct ectasia and obvious periductal inflammatory changes.the clinical manifestations of pcm are breast mass and inflammatory changes,which should to be distinguished with advanced breast cancer and inflammatory breast cancer.the surgical treatment is extensive resection.the major pathological changes are inflammatory reaction and multiple abscesses in breast tissue.conclusion:mde and pcm are significant different,and they are different progressing stage of disease,thus the two diagnoses are independent.

目的：分析乳腺导管扩张症和浆细胞性乳腺炎临床上的差异。提出各自独立诊断的论据。方法：结合24例乳腺导管扩张症和28例浆细胞性乳腺炎对两病的临床症状、鉴别诊断、手术治疗、病理结果进行比较分析。结果：乳腺导管扩张症临床表现为乳头溢液和乳腺肿物，主要与乳腺肿物和早期乳腺癌鉴别诊断，手术以局部切除多见，病理表现为导管扩张及导管周围明显炎性改变。浆细胞性乳腺炎临床表现为乳腺肿物和炎性改变，主要与晚期乳腺癌和炎性乳腺癌鉴别诊断，手术切除的范围较大，病理变化以乳腺组织的炎性反应和多发性脓肿为主。结论：乳腺导管扩张症和浆细胞性乳腺炎有明显的差异，应作为这两种疾病独立诊断。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Study the bioactivity of the n-HA/PA66 composite and the effects it would be to body's metabolism of calcium and phosphorus ion in vivo.(3) Study the osteo-conductivity and the ability to repair bone defect of the porous n-HA/PA66 composite and the feasibility use it as the scaffold of bone tissue engineering. Objects and Methods as follows: 1.To evaluate the biocompatability of nano-hydroxyapatite crystals and polyamide composite (n-HA/PA66) with the L929 cells.To proceed the morphological observation and take pictures of L929 cells after 1d,2d,4d,and 7d of co-cultured with extract of n-HA/PA66 ,and direct contact with n-HA/PA66.To determine light absorbtion value of every hole under 500 nm with enzyme linked immunity instrument after 1 d,2 d,4 d,and 7 d of contact of n-HA/PA66 extract with L929 cells,and direct contact with n-HA/PA66.In the meanwhile calculate the relative multiplication rate of cells,and evaluate them by six degree tests for cytotoxicity. To investigate the acute and chronic toxic reaction on the whole body induced by the new nano-hydroapatite crystals and polyamide composite(n-HA/PA66)after implanting in vivo and its effects on partial constitution of animal organs after implanting in vivo,and evaluate the potential and degree of subcuticular stimulation reaction.

本实验主要由以下三部分组成：一、n-HA/PA66 复合材料在动物体内、体外的生物相容性及生物安全性评价二、n-HA/PA66 复合材料植入动物体内的生物活性及近期对机体钙、磷代谢影响的实验研究三、网孔 n-HA/PA66 复合材料作为支架修复兔桡骨节段缺损的动物实验研究主要研究目标及方法如下：参照 GB/T16886.5-1997-ISO 10993-5：1992《医疗器械生物学评价细胞毒性试验体外法》之评价标准和要求，采用规定的 L929 细胞(小鼠结缔组织成纤维细胞)，分别经直接接触和材料浸提液与细胞共培养等方式对 n-HA/PA66 复合材料进行细胞毒性测试，采用细胞形态观察法观察两种细胞各组在 24h、48h、72h、5 天后各时相点的细胞形态学变化，并在显微镜下照相，从而对细胞与材料的生物相容性进行定性评价；同时采用细胞生长抑制法，以酶标仪定量测定评价各组 1，2，4，7 天 L929 细胞的相对增殖率，以定量测定并判别材料对细胞的毒性程度。

In vivo, CXCR4-A549 and A549 cells were injected subcutaneously into nude mice(each group in 6).Four weeks later the mice were scarified and the tumors in situ and the lungs were taken out to be examined histologically.(3) Th cell cycle distribution was measured by flow cytometry to analyse the effect of SDF-1/CXCR4 in the cell proliferation and atoposis. The changes of the intracellular calcium concentration after SDF-1 activation were aslo measured by flow cytometry. Western blot was used to anlayse the phosphorylation of AKT and ERK, and the expression of MMP-2 and VEGF-C.

3为探讨SDF-1/CXCR4在肺癌侵袭和转移中的作用机制：①利用流式细胞仪检测CXCR4-A549与A549两组细胞的增殖和凋亡，分析上调CXCR4的表达后，对细胞生长周期的影响；②利用流式细胞仪检测两组细胞钙离子内流强度，分析SDF-1/CXCR4对钙离子通道活性的调控；③采用Western blot实验检测两组细胞PI3K/AKT、MAPK/ERK相关信号通路的活性，分析SDF-1/CXCR4可能激活的下游信号分子；④采用Western blot和RT-PCR检测两组细胞肿瘤转移相关基因VEGF-C、MMP-2的表达，探讨SDF-1/CXCR4与VEGF-C、MMP-2的相互作用关系。

Methods: By the adherence , to isolate the bone marrow mesenchymal stem cells and adipose mesenchymal stem cells , to compare the characteristics of the two cells , including obtain the quantity of the cells 、 the morphos of the cells 、 the rate of adherence 、 cell kinetics 、 cell surface marker .

利用细胞贴壁性对骨髓间充质干细胞和脂肪间充质干细胞进行分离，比较两种细胞的情况，包括(1)单位质量组织获得两种细胞的量、(2)细胞形态、(3)细胞贴壁率、(4)细胞动力学、(5)细胞表面标记。

In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我们在取得两种不同品系小鼠的卵丘卵母细胞复合体后，先将卵丘卵母细胞复合体置于含有多种生长因子和激素的MEM培养液中培养6小时，此时卵母细胞已进入第一次减数分裂的前中期，并且在DIC倒置显微镜下可以看到浓缩的染色体，用Polscope可以发现明显的纺锤体，借助这种显微镜通过显微操作将两种不同品系小鼠来源的卵母细胞的MI纺锤体进行互换，经过三次直流电脉冲作用后，分别有91.6％的胞质—MI核质体对融合，经过进一步的培养后，超过80％的重组卵母细胞排出第一极体，体外受精后分别有79％和77％的重组卵形成双原核，受精后的胚胎在KSOM胚胎培养液中体外培养4天后，超过60％的胚胎发育至囊胚。

与两细胞的相关的网络解释 [注：此内容来源于网络，仅供参考]

apical cell：顶端细胞

无论是主枝或是短枝,顶端都有一个半球形细胞,叫做顶端细胞(apical cell),植物的生长即由顶端细胞不断分裂形成的. 顶端细胞横分裂,形成两个子细胞,上面的子细胞继续保持顶端细胞的作用(见图). 绿藻和高等植物之间有很多相似之处,

Hair cell：毛细胞

2)毛细胞(hair cell)为感受声波刺激的感觉上皮细胞,有内、外两群. 内毛细胞形似瓶状,约有3500个,成单行排列于内指细胞的凹陷内. 外毛细胞呈柱形,约有12 000-20 000个,成3-5行排列,细胞底部嵌接于外指细胞的凹陷内.

rods：视杆细胞

光线通过其中8层,被另外两层的视杆细胞和视锥细胞的光接收器吸收:视杆细胞(rods)负责低分辨率的、单色的、夜间的视觉. 视锥细胞(cones)负责高分辨率的、彩色的、白天的视觉. 它花费25ms把光转成电信号,再送给大脑. 光学传感器有各种途经.

primary spermatocyte：初级精母细胞

(2)初级精母细胞(primary spermatocyte)这类细胞的体积比精原细胞大. 细胞核的DNA经过复制后,细胞进行第一次成熟分裂,分裂后的细胞染色体的数目减少一半,所形成的两个次级精母细胞,一个含有22条常染色体和一条X染色体,另一个含有22条常染色体和一条Y染色体.

amphophilic：两染的,双染的,兼染酸性及碱性色素的

\\"双染性细胞,中性,嗜两性\\",\\"amphophil\\" | \\"两染的,双染的,兼染酸性及碱性色素的\\",\\"amphophilic\\" | \\"安比西林\\",\\"ampicillin\\"

bicellular：两室的

bice | 灰蓝色, (泛指)绿色 | bicellular | 两室的 | bicellulate | 具二细胞的

diplostichous：两列的,两行的

diplospory 倍数孢子形成,种细胞无孢子生殖 | diplostichous 两列的,两行的 | diplostreptococcus 双链球菌

heterogametic：两种胚细胞的

heteroecism 转主寄生现象 | heterogametic 两种胚细胞的 | heterogamous 世代交替的

heterogametic：形成两种胚细胞的; 异形配子的 (形)

heterogamete 异形配子 (名) | heterogametic 形成两种胚细胞的; 异形配子的 (形) | heterogamous 异形配子的; 世代交替的; 异配生殖的 (形)

chromophil cell：嗜色细胞

胞两大类.嗜色细胞(chromophil cell)又分为嗜酸性细胞和嗜碱性细胞两种.应用电镜免疫细胞化学技术.可观察到各种腺细胞均具有分泌蛋白类激素细胞的结构特点.而各类腺细胞胞质内颗粒的形态结构.数量及所含激素的性质存在差异.可以此区分各种分泌不同激素的细胞.并以所分泌的激素来命名.▲2.中间部 人的中间部(pars intermedia