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The somatic hybrids usually showed specific bands and combined all the bands of both parents. In combinations, some new bands were detected, and occasionally specific bands of parents were lost, which indicated that chromosome recombination and exchange happened after fusion. Additionally, in some combinations, specific bands from leaf parent were detected, which revealed the plants were of putative hybrid origin, because leaf protoplast cannot redifferentiate by both single culture and co-culture.
体细胞杂种一般表现为具有双亲的特征带,且综合双亲的所有带,但在部分杂种中检测到了双亲都没有的新带,也发现有丢失亲本的特征带或共有带的现象,说明融合后染色体发生了重组和交换;有的引物只检测到叶肉亲本的特征带,根据柑橘叶肉细胞无论单独培养还是共培养均不能再生的事实,可推测其为体细胞杂种或二倍体叶肉亲本型胞质杂种。
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azeotropic distillation:共沸蒸馏
有些有机污染物在受热情况下不太稳定,加热之后,要不挥发,要不水解为水溶性物质从而降低毒性. 当然,也许有的污染物在受热情况下,依然不动,那是没办法的事情. 还有,在水沸腾过程中,可以用共沸蒸馏(Azeotropic distillation )去掉能挥发的有机污染物.