上切式

基本解释 (translations)

upcut

与上切式相关的网络例句 [注：此内容来源于网络，仅供参考]

In application, it would use trim problem of cantilever wall for Taiwan Highway Bureau as an example to establish the optimal requirement layout of steel bar by different retaining wall height. It would decrease scrap through appropriate trim plan of steel bar and the efficiency is obvious.

应用上，本文以台湾省公路局悬臂式挡土墙设计标準图主筋裁切為例，尝试建立不同挡土墙高度之主筋裁切最这钢筋需求配置，试想若能透过这当钢筋裁切计画使残料数量降低，其间的效益不可忽视。

JWQJ-3000 all-purpose cutting machine, QWQJ-2000 light all-purpose cutting machine, light cutting machine, raising peak manner diamond frame saw, all standers of millers for granite, etc. are our main products which enjoy high reputation at home and abroad.

其主要产品有： JWQJ-3000万能切机，QWQJ-2000轻便型万能切机，轻便切机，上顶式金刚石框锯，花岗石各种规格的磨头等。

The technical proposal includes a frame (9), a suspension frame (1) on the frame (9), a depth wheel (7) and a power system. The invention is characterized in that the power system is connected with one or more than one crank and rocker mechanisms provided with one or more than one cutters(6). The cutter (6) is parallel to the advancing direction of the multifunctional punctuate, strip typed grass root cutter.

技术方案包括机架(9)，位于机架(9)上的悬挂架(1)，限深轮(7)和动力系统，其特征在于，与该动力系统相连接有一个或一个以上的曲柄摇杆机构，在该曲柄摇杆机构上安装了一把或一把以上的切刀(6)，所述切刀(6)与本多功能点、条式牧草切根机的前进方向平行。

On the basis of analyzing the industrial application, marketing shears and development actuality and trends of the shear homogenizer both home and abroad, we discussed the feasibility of its application in different industries.

本文首先介绍了剪切式均质机国内外发展现状与趋势，分析了剪切式均质机的工业应用与市场地位，并从理论上探讨了剪切式均质机应用于工业领域及众多行业的可行性。

Zhili CUTTING MACHINERY's facty area funded in WANJIANG industrial district, DONGGUAN. ITS products including double-cylinder with four columns double pillars cutter, sptive plastic cutter, automatic rocker cutter, level cutter, all kinds of making shoes line.

智力机械在大陆的生产基地设在东莞万江，产品包括精密四柱油压裁断机、吸塑包装裁断机、自动送料裁断机、直切机、平切机、发泡机、上胶机、吸塑机、平面式裁断机、摇臂裁断机、龙门油压裁断机、铆钉机、折边机及皮鞋生产线等。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Premaxillary incisor band, outside front distraction mask, and distraction rubber band were self-made.

自制上切牙带环、外置式前牵引面具、牵引橡皮圈。

From the original signal of"ladder type" undercut, it is difficult to find out the exception, through observing the undercut signal by means of the"focalizing scanning"of wavelet transform in time-frequency domain, the fault features are accurately extracted. The results reveal that this method has flexibility to the variation of tool diameters, tooth of cutter and cutting parameters.

在&阶梯型&过切的原始信号上几乎看不出信号有明显异常现象，在时频空间采用小波分析方法&聚焦式&扫描观察&阶梯型&过切信息，能准确地提取过切的故障特征，研究表明，该方法对刀具直径、刀具齿数及切削参数的改变有较强的适应性。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

In such case,the bilirubin was adsorbed on the silver colloid particle with the two planar pyrromethenone groups intercalated into the globe silver colloid particle,however,the biliverdin might lie flat on the surface of silver colloid with syn-synsyn conformation.

通过对其谱带的指认分析，表明胆红素形成了三对内氢键，并以其两个吡咯亚甲基酮环沿银胶颗粒表面切向方向镶嵌在银胶颗粒上，而胆绿素则以顺式-顺式-顺式构型平躺吸附于银胶颗粒表面。

与上切式相关的网络解释 [注：此内容来源于网络，仅供参考]

Cheltenham：切尔滕纳姆

帽子是在店内的作坊里定型,勾出棱线的,然后加上切尔滕纳姆(Cheltenham)式的内衬带(在没那么讲究的店里,该衬带也许被称作吸汗带)和外饰带. 他们跟我说,帽子上的装饰带,可按顾客兴之所至,选择颜色,从梅花到圆点都行.

upcut：上切式

upcurve 上升曲线 | upcut 上切式 | updatabledata 可更新数据

downcut shears：上切式剪切机

downcomer 降气管 | downcut shears 上切式剪切机 | downgate 直浇口

downgate：直浇口

downcut shears 上切式剪切机 | downgate 直浇口 | downhand position 平焊位置

up-cutting guillotine shears：上切闸刀式剪切机

up-cut shears 上切式剪切机 | up-cutting guillotine shears 上切闸刀式剪切机 | up-down collar-press 上下领压机

up-cut lever shears：上切杠杆式剪切机

up-converting laser 升频转换激光器 | up-cut lever shears 上切杠杆式剪切机 | up-cut shears 上切式剪切机

MOLARS：磨牙

牙式 根据牙的形态和功能可把牙划分为切牙(incisors)、尖牙(canine teeth)、前磨牙(premolars)和磨牙(molars)(图5-6A). 切牙用以咬切食物,尖牙和磨牙有撕裂食物和研磨食物的功能. 不同的牙按一定的规律排列成上、下两列,