were it not for

were it not for的基本解释

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若不是有...的话, 若非

与were it not for相关的网络例句 [注：此内容来源于网络，仅供参考]

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

Thx! Demi Lovato - Everything You'Re Not I've been bruised and I've been broken Can't believe that I put up with all this pain I've been used and I was chocking on the promise I would never fall again I used to sing to your twisted symphony The words that had me trapped inside your misery But now I know The reason why I couldn't breathe 'Cause all I want is everything you're not So go ahead and slam the door 'Cause you can't shut me out And no i don't, I don't care what you say 'Cause all I really, all I really want Is everything you're not Everything you're not not not Your darkness was my weakness but it turns out That it only went so deep,deep A meaningless diversion that is all that you Ever meant to me And I am done with your twisted symphony The words that had me sound like stolen poetry I tore the pages and I can finally breathe 'Cause all I want is everything you're not So go ahead and slam the door 'Cause you can't shut me out And no i don't, I don't care what you say 'Cause all I really, all I really want Is everything you're not I want a gentleman who treats me like a queen I need respect, I need love nothing in between I will not spell it out for you if you can't see 'Cause you're not worthy, you don't deserve me A now I'm gone Everything you're not, not, not Everything you're not, not, not 'Cause all I want is everything you're not So go ahead and slam the door 'Cause you can't shut me out And no i don't, I don't care what you say 'Cause all I really, all I really want Is everything you're not Never gonna break my heart again Never gonna see your face again Never wanna feel this way again Never gonna break my heart again Never gonna see your face again Never wanna feel this way again

我一直伤痕累累，我已经被打破了不敢相信我忍受这一切痛苦我一直在使用上，我是会让人窒息的承诺我决不会再次下跌我从前对你们扭曲的交响乐我的言语，困在里面的痛苦但是现在我知道了为什么我不能呼吸&我想要的一切，你不是来吧，砰的一声关上门因为你可以相信我没有，我不知道，我不在乎你说什么&我真的，我真正想要的每件事都是你不是这样吗你所说的一切都是不不不你是我的弱点，但黑暗那只是走了如此之深，深这是一个无意义的导流你一切所有的曾经想我我需要你的扭曲的交响乐这让我的话听起来像是偷来的诗歌我撕了，我终于可以呼吸的页面&我想要的一切，你不是来吧，砰的一声关上门因为你可以相信我没有，我不知道，我不在乎你说什么&我真的，我真正想要的每件事都是你不是这样吗我想要一个绅士一样对待我的王后我需要尊重，我需要爱没什么我不会拼写出来给你，如果你不能看见因为你不值得的，你不值得我现在我是一去不复返了你所说的一切都是不、不、不你所说的一切都是不、不、不&我想要的一切，你不是来吧，砰的一声关上门因为你可以相信我没有，我不知道，我不在乎你说什么&我真的，我真正想要的每件事都是你不是这样吗永远不会伤透了我的心永远要看到你的脸永远要有这样的感觉有些别扭，凑合着看吧

Major results were summarized as follows:(1) A total of 58 tree species from 53 genera in 36 families among which 8 species were Rosaceae, 5 species were Liliaceae, 5 species were Eriaceae, 3 species were Ranunculaceae, 3 species were Compositae, 2 species were Cupressaceae, 2 species were Caprifoliaceae, 2 species were Umblliferae, and other 28 tree species were only one receptively. These were recorded in the 8 plots of the A. georgei var. smithii forest;(2) Number of families, genera, species and Margalef index correlated negatively with altitude (P.05), with a peak at 3600 m.(3) Shannon-Wiener index correlated negatively with altitude (P.01), and maintained stable at the altitudes between 3700~4100 m, evenness with altitudes, however, this trend was insignificant.(4) Jaccard index increased sharply with increasing altitude at the altitudes between 3600~4100 m, and was lower between different vegetation types at the altitudes between 4100~4200. Cody index β(subscript c decreased with an increasing altitude, but there were 2 troughs between 4000~4100 m and 4200~4300 m.(5) Maximum tree height H(subscript max and HH(subscript max=37 mcorrelated negatively with altitude (P.05); but basal area and BA BA(subscript max=5.3m^2 correlated with altitudes, however, this trend was insignificant.

结果表明：(1)在调查的8个急尖长苞冷杉林样地内共有植物58种，分属于36科53属，其中植物较多的科有蔷薇科8种、百合科5种、杜鹃花科5种、毛茛科3种、菊科3种、柏科2种、忍冬科2种、伞形科2种，其余的28科各只有1个种；(2)物种科、属、种数、Margalef指数D(下标 M与海拔存在显著的负相关性(P.05)，在分布急尖长苞冷杉最低海拔3600m处出现物种丰富度的最大值；(3)多样性指数与海拔之间有极显著的负相关性(P.01)，并且在3700~4100m之间多样性指数保持稳定；均匀度指数与海拔梯度之间存在负相关性，但不显著；(4)Jaccard指数C(下标 j在海拔3600~4100m随海拔的升高而升高，在生境过渡带的4100~4200m之间Jaccard指数C(下标 j较低；Cody指数β(下标 c随海拔的升高呈下降的趋势，但在4000~4100m和4200~4300m 海拔区间出现2个低谷；(5)最大树高H(下标 max和最大胸径DBH(下标 max与海拔之间存在显著负相关性(P.05)；胸高断面积之和和立木密度与海拔之间存在负相关性，但不显著。

与were it not for相关的网络解释 [注：此内容来源于网络，仅供参考]

were it not for：若不是,假如没有

wear and tear 磨损和毁坏 | were it not for 若不是,假如没有 | What a vision! 多壮观!

were it not for：若不是有

wept 哭泣 | were it not for 若不是有 | were 是

were it not for：要是没有...的话; 要不是

were: be的过去式(陈述语气用于复数, 虚拟语气用于单数及复数) | were it not for ... 要是没有...的话; 要不是... | 17 I mean, not as far as I'm concerned. 我想到目前为止和我无关.

were it not for：若非,苟无

777.well off 富裕,兴盛. | 778.were it not for 若非,苟无. | 779.what is more 并且,又.

except for apart from;were it not for：除...外

The castle has a lovely situation on a hill. 城堡座落在山上,环境优美. | except for apart from;were it not for 除...外 | v. pass on (news, information, feelings, etc.) to sb. or sth. 传达(消息、感情等...