were [wə:]
- were的基本解释
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be的过去式
- I wish it were so.
- 我巴不得这样。
- If I were you, I would stay.
- 倘若我是你,那我就留下。
- 相似词
- were的同义词
- v. be
- 与were相关的情景对话
- 衣食住行 / 肯定表达
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A:
If I were in your position, I wouldn’t give up an inch.
如果我处在你的位置,我会存不不让。
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B:
I think that’s an excenllent proposal.
我觉得这是个出色的建议。
- 衣食住行 / 否定表达
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A:
If I were in your position, I wouldn’t give up an inch.
如果我处在你的位置,我会寸步不让。
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B:
I regret to say I can’t accept your suggestion.
很遗憾,我不能接受你的建议。
- 商务外贸 / 缺货
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A:
Were you able to quote on all the items we need ?
我们需要的每个项目你都能报价吗。
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B:
No, not all of them .
不,没办法全部。
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A:
Oh ?why not ?
哦,为什么?
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B:
We aren‘t able to supply the third ad fifth items .
第三及第五项目,我们没有货供应。
- 更多 网络例句 与were相关的网络例句 [注:此内容来源于网络,仅供参考]
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RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .
结果 成功构建突变人CD59的真核细胞表达系统:运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞:用含有400ug/mlG418的F12培养基培养14天,筛选出稳定阳性表达克隆,RPMI1640培养基扩增获得稳定表达细胞株,并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02,表达率分别为53.7%、54.5%;应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株;RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株,裂解细胞得到CD59蛋白质;通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达;50mM核糖培养72小时,获得突变人CD59糖化细胞株,BCECF染料释放试验结果显示,PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低,未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。
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Major results were summarized as follows:(1) A total of 58 tree species from 53 genera in 36 families among which 8 species were Rosaceae, 5 species were Liliaceae, 5 species were Eriaceae, 3 species were Ranunculaceae, 3 species were Compositae, 2 species were Cupressaceae, 2 species were Caprifoliaceae, 2 species were Umblliferae, and other 28 tree species were only one receptively. These were recorded in the 8 plots of the A. georgei var. smithii forest;(2) Number of families, genera, species and Margalef index correlated negatively with altitude (P.05), with a peak at 3600 m.(3) Shannon-Wiener index correlated negatively with altitude (P.01), and maintained stable at the altitudes between 3700~4100 m, evenness with altitudes, however, this trend was insignificant.(4) Jaccard index increased sharply with increasing altitude at the altitudes between 3600~4100 m, and was lower between different vegetation types at the altitudes between 4100~4200. Cody index β(subscript c decreased with an increasing altitude, but there were 2 troughs between 4000~4100 m and 4200~4300 m.(5) Maximum tree height H(subscript max and HH(subscript max=37 mcorrelated negatively with altitude (P.05); but basal area and BA BA(subscript max=5.3m^2 correlated with altitudes, however, this trend was insignificant.
结果表明:(1)在调查的8个急尖长苞冷杉林样地内共有植物58种,分属于36科53属,其中植物较多的科有蔷薇科8种、百合科5种、杜鹃花科5种、毛茛科3种、菊科3种、柏科2种、忍冬科2种、伞形科2种,其余的28科各只有1个种;(2)物种科、属、种数、Margalef指数D(下标 M与海拔存在显著的负相关性(P.05),在分布急尖长苞冷杉最低海拔3600m处出现物种丰富度的最大值;(3)多样性指数与海拔之间有极显著的负相关性(P.01),并且在3700~4100m之间多样性指数保持稳定;均匀度指数与海拔梯度之间存在负相关性,但不显著;(4)Jaccard指数C(下标 j在海拔3600~4100m随海拔的升高而升高,在生境过渡带的4100~4200m之间Jaccard指数C(下标 j较低;Cody指数β(下标 c随海拔的升高呈下降的趋势,但在4000~4100m和4200~4300m 海拔区间出现2个低谷;(5)最大树高H(下标 max和最大胸径DBH(下标 max与海拔之间存在显著负相关性(P.05);胸高断面积之和和立木密度与海拔之间存在负相关性,但不显著。
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Compared with control,①the mean tumor weight of H22 of SXKA Granules three dose groups were decreased significantly(P.01,P.05),and the mean inhibition rates of SXKA Granules 20、10 g/kg dose groups were above 30%;②the mean tumor weight of S180 of SXKA Granules three dose groups were decreased significantly(P.01),and the mean inhibition rates of SXKA Granules 20、10 g/kg dose groups were above 32%;③the mean tumor weight of EAC of SXKA Granules three dose groups were decreased significantly ( P.01, P.05),and the mean inhibition rates of SXKA Granules 20 g/kg dose groups were above 38%;④the mean tumor weight of Lewis carcinoma of SXKA Granules three dose groups were decreased significantly(P.01, P.05),and the mean inhibition rates of SXKA Granules 20、10、5 g/kg dose groups were above 36%;⑤the mean tumor weight of W256 of SXKA Granules three dose groups were decreased significantly ( P.01, P.05),and the mean inhibition rates of SXKA Granules 20、10 g/kg dose groups were above 32%;⑵Compared with control,SXKA Granules 20、10 g/kg dose groups had extended the survial time of the P388-bearing mice respectively(P.01),and the mean prolong rates of SXKA Granules 20、10 g/kg dose groups were above 50%;⑶Compared with S180-bearing group, SXKA Granules 20、10 g/kg dose groups could increase the weight of thymus and spleen, Spleen index and thymus index were increased, SXKA Granules 5 g/kg dose group could increase thymus index(P.05);⑷As Compared with control group, SXKA Granules 20、10 g/kg dose groups could improve mouse serum half hemolysis value depressed by transplanted tumor dramatically(P.01), which revealed the SXKA granules could improve the mouse humoral immunity system;⑸SXKA Granules 20 g/kg dose group could increase of englobe indexαon S180-bearing mice remarkably(P.01), which indicated the SXKA Granules could improve their cellular immunity system.
对荷W256大鼠,生兴克癌冲剂20、10、5 g / kg三组的平均瘤重明显低于对照组(P.01,P.05),生兴克癌冲剂20、10 g / kg组的平均肿瘤抑制率均大于32 %;⑵与空白对照组相比,生兴克癌冲剂20、10 g/ kg能显著地延长移植小鼠白血病P388小鼠的存活天数(P.01),生兴克癌冲剂20、10 g/ kg对荷白血病P388小鼠生命延长率均在50%以上;⑶与S180荷瘤组相比,生兴克癌冲剂对荷瘤鼠的免疫器官重量、胸腺指数和脾指数有一定的提高趋势,其中生兴克癌冲剂5 g / kg组对荷瘤小鼠的胸腺指数有一定的提高作用(P.05);⑷与S180荷瘤组相比,生兴克癌冲剂20、10 g /kg组可提高由荷瘤引起的小鼠血清半数溶血素值的降低(P.01),表明其可提高荷瘤小鼠体液免疫功能;⑸与对照组相比,生兴克癌冲剂20 g /kg组可提高荷S180肉瘤小鼠的免疫吞噬系数α值(P.01),表明其可提高荷瘤小鼠细胞免疫功能。
- 更多网络解释 与were相关的网络解释 [注:此内容来源于网络,仅供参考]
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were:是
1064 . well 很好、令人滿意地 | 1065 . were 是 | 1066 . west 西部
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were:是(are的过去式)
38. other 其它的 | 39. were 是(are的过去式) | 40. all 全部的
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were:(动词are的过去式)
remember--vt.记得;想起 | were--(动词are的过去式) | hope--vt.希望
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were:沃哷
Weigute 韦古特 | Were *沃哷 | Wase *斡色
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Were through:我们完了
87. Youll be sorry. 你会后悔的. | 88. Were through. 我们完了! | 89. Look at the mess youve made! 你搞得一团糟!
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