used to do

used to do的基本解释

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过去常常做某事

与used to do相关的网络例句 [注：此内容来源于网络，仅供参考]

I dance around this empty house Tear us down Throw you out Screaming down the halls Spinning all around and now we fall Pictures framing up the past Your taunting smirk behind the glass This museum full of ash Once a tickle Now a rash This used to be a funhouse But now it's full of evil clowns It's time to start the countdown I'm gonna burn it down down down I'm gonna burn it down 9, 8, 7, 6 5 4, 3, 2, 1, fun Echoes knocking on locked doors All the laughter from before I'd rather live out on the street Than in this haunted memory I've called the movers Called the maids We'll try to exorcise this place Drag my mattress to the yard Crumble tumble house of cards This used to be a funhouse But now it's full of evil clowns It's time to start the countdown I'm gonna burn it down down down This used to be a funhouse But now it's full of evil clowns It's time to start the countdown I'm gonna burn it down down down I'm gonna burn it down 9, 8, 7, 6 5 4, 3, 2, 1, fun Oh, I'm crawling through the darkest home My key don't fit my life no more I'll change the drapes I'll break the plates I'll find a new place Burn this fucker down do do do do dodo do do do do do dodo do do do do do dodo do do do do do dadadada do do do do dodo do (9, 8, 7, 6 5 4, 3, 2, 1) do do do do dodo do do do do do dodo do do do do do dodo doo This used to be a funhouse But now it's full of evil clowns It's time to start the countdown I'm gonna burn it down down down This used to be a funhouse But now it's full of evil clowns It's time to start the countdown I'm gonna burn it down down down I'm gonna burn it down

本人舞蹈解决这个空房子撕裂我们失望你驱逐出去尖叫了大厅纺纱各地，现在我们属于图片框过去您的嘲弄微笑背后的玻璃该博物馆完全灰一旦痒现在皮疹这曾经是一个funhouse 但现在是充满了邪恶的小丑现在是时候开始倒计时我要去烧伤跌下来了我要去刻录下来 9 ， 8 ， 7 ， 6 5 4 ， 3 ， 2 ， 1 ，乐趣回声敲锁车门所有的笑声之前我宁愿住在街道上进行比这个闹鬼记忆我已经称为推动者所谓的女佣我们会尝试傩这个地方拖曳我床垫的码崩溃暴跌众议院卡这曾经是一个funhouse 但现在是充满了邪恶的小丑现在是时候开始倒计时我要去烧伤跌下来了这曾经是一个funhouse 但现在是充满了邪恶的小丑现在是时候开始倒计时我要去烧伤跌下来了我要去刻录下来 9 ， 8 ， 7 ， 6 5 4 ， 3 ， 2 ， 1 ，乐趣噢，我最黑暗的爬行通过主页我的钥匙不适合我的生活没有更多我会改变窗帘我要打破盘子我会找到一个新的地方烧伤这个笨蛋下跌做这样做这样做渡渡鸟做这样做这样做渡渡鸟做这样做这样做渡渡鸟这样做这样做dadadada 这样做这样做渡渡鸟做（ 9 ， 8 ， 7 ， 6 5 4 ， 3 ， 2 ， 1 ）做这样做这样做渡渡鸟做这样做这样做渡渡鸟这样做这样做渡渡鸟斗这曾经是一个funhouse 但现在是充满了邪恶的小丑现在是时候开始倒计时我要去烧伤跌下来了这曾经是一个funhouse 但现在是充满了邪恶的小丑现在是时候开始倒计时我要去烧伤跌下来了我要去刻录下来

When the mind apprehends the essence of a thing (quod quid est; tò tí en enai), the external object is perceived without the particular notes which attach to it in nature, and it is not yet marked with the attribute of generality which reflection will bestow on it.

当铭记apprehends的本质的东西（狱吏嚼1-5 ；德州仪器恩enai ），外部对象被认为没有特别注意到它重视它的性质（中存在singularibus ），这是没有标明属性一般性的反映将赋予它

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

与used to do相关的网络解释 [注：此内容来源于网络，仅供参考]

used to do：过去常常

他不能和朋友们相处好,老师不喜欢他,应该是他一直(always)需要洗澡4.B Teddy是一个失去母亲而同时失去母爱的小男孩,在之前(before)应该是非常好的学生8.A 是母亲过去常常(used to do)的动作, 而现在母亲去世了,

used to do：过去常做

make a good football player 成为一名出色的足球队员 | used to do 过去常做 | lose to somebody 输给某人

used to do：过去做某事

go on doing ... 继续做某事 | used to do ... 过去做某事 | be used to doing ... 习惯做某事

used to do：过去常常做

12.as well as 也;还;而且 | 13.used to do 过去常常做...... | 14.at least 最少;至少

used to do sth：过去常常

used to sth. 习惯于 | used to do sth. 过去常常 | wait for 等候,等待