unmanipulated
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Methods : Liver DCs were isolated without expansion in cytokines from human liver allowing us to study unmanipulated tissue-resident DCs ex vivo .
利用直接分离的人肝脏树突状细胞,研究肝脏常驻树突细胞。
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However, most reports on these aspects have been limited to the unmanipulated cells. The majar goals of the second part of this study is to thoroughly explore the effect of ex vivo expansion to the homing-related potential of UCB CD34〓 cells.
基于该领域的研究现状和第一部分的实验结果,第二部分在不同的扩增时点,将CD34〓细胞从扩增产物中再次纯化出来,从动态的角度观察了扩增过程中UCB CD34〓细胞归巢相关细胞粘附分子的表达、粘附功能、趋化功能和趋化因子受体CXCR4表达的变化,从而评价细胞因子介导的体外扩增对UCB HSPC体外归巢相关功能的影响。
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METHODS: Liver DCs were isolated without expansion in cytokines from human liver allowing us to study unmanipulated tissue-resident DCs ex vivo.
从人肝脏中没有表达细胞因子肝树状突细胞被分离出来,允许我们在体外研究未经过处理的组织原位的树状突细胞。
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METHODS: Lier DCs were isolated without expansion in cytokines from human lier allowing us to study unmanipulated tissue-resident DCs ex io.
从人肝脏中没有表达细胞因子肝树状突细胞被分离出来,允许我们在体外研究未经过处理的组织原位的树状突细胞。
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Three animals randomly selected from three groups were killed on Day 0 to determine the baseline of PLA2 activity within unmanipulated rat sciatic nerve; other 39 animals underwent operation for model-making of acute sciatic (Maves-modified Bennet-Xie model) on right sciatic nerves, while Sham operations were performed on left sciatic nerves.
第0天,每组随机抽取1只共3只大鼠处死,测量双侧坐骨神经节段磷脂酶A2活性为基线,其余39只大鼠右侧坐骨神经制作急性坐骨神经痛模型(Maves改良的Bennet-Xie模型),对侧行Sham手术;第7天,每组随机抽取3只处死,测量治疗前模型和Sham坐骨神经节段的磷脂酶A2的活性;第14天,剩余大鼠处死后测量各组模型和Sham坐骨神经节段的磷脂酶A2的活性。
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