tunnel effect

tunnel effect的基本解释

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[电子]隧道效应

与tunnel effect相关的网络例句 [注：此内容来源于网络，仅供参考]

The entrance section of FuXi Double-arched Tunnel is modeled in the fact of factual shallow buried and unsymmetrical pressure condition. Adopting the three heading construction method to simulate twelve kinds of construction projects, confirm reasonable construction orders and left and right tunnel face interval, systematically analyze and research the changing law of the tunnel deformation and surrounding rock stress in soft and cracked condition of surrounding rock,bring forward pertinent advices against construction disease; Build the deep buried tunnel model in middle section of tunnel , separately adopt the central heading bench cut benching method and the central heading full cross method to simulate ten kinds of construction projects, establish the reasonable construction orders, sidestep length and tunnel face interval, which are due to the deep buried tunnel, systematically analyze and research the change law of the tunnel deformation and surrounding rock stress in the section with good surrounding rock condition, bring forward the reasonable construction projects which can make sure of construction safety and save construction time; Because the central wall is disturbed many times during constructing process, its displacement and stress distribution is very complex.

富溪隧道进口段依照实际的浅埋偏压状况建立模型，采用三导洞法开挖模拟了12种方案，确定了合理的施工顺序和左右主洞掌子面错距，系统地分析研究了软弱破碎围岩浅埋偏压段施工的隧道变形和围岩应力变化规律，提出了防止施工病害的针对性建议；洞身段按深埋隧道建立模型，分别采用中导洞台阶法和中导洞全断面法模拟了10种方案，确立了适合深埋隧道的合理施工顺序、台阶长度和掌子面错距，系统分析研究了围岩较好深埋段施工的隧道变形和围岩应力变化规律，提出了兼顾施工安全和节省工期的合理施工方案；由于受到多次扰动，中隔墙的位移和应力变化规律较为复杂，通过对各种方案的对比分析，得出了中隔墙在施工不同阶段的位移变化规律、应力变化规律和应力分布规律，尤其对于浅埋偏压段隧道施工时中隔墙位移和应力变化规律做了深入研究，提出了合理的施工建议。

The selection of geological prospecting in different stages(initial-prospecting, detailed-prospecting)(2)the selection of geological prospecting in the deep-laid and shallow-laid of tunnel project (3)the selection of geological prospecting of tunnel projecting in different geological conditions Finally, the author summed up main problems needing to be solved in tunnel engineering geological prospecting as follow:(1)differentiate stratum and find out the depth and thickness of different stratums in the tunnel section (2)find out geological structure and existence situation of fault, broken zone and weak plane (3)find out weathering level of bed rock to classify surrounding rock combining speed materials (4)differentiate stratum structure of the entrance of tunnel to judge the stability of it (5)find out existence situation and performance of ground water (6)situations of other harmful geological phenomenon In view of above problems and classification of surrounding rock, the author showed principles of rational choosing of methods in geological prospecting and counted disperse range of physical character parameter value in different petrosal character, getting achievements as follow:(1)summarization of effect, merits and demerits of different geological prospecting methods in the tunnel geological investigation (2)method selection and arrangement principle of tunnel engineering geological prospecting in different geological conditions and prospecting stages (3)According to previous experience and cases, the author concluded the物性parameters most in use ,and showed the basis of selecting geological prospecting methods in different geological conditions.

论文中用几个应用实例来说明：(1)不同勘察阶段物探工作的选用；(2)深埋、浅埋隧道工程物探工作的选用；(3)不同地质条件隧道工程物探工作的选用。最后总结归纳出隧道工程物探所需要解决的问题主要有：(1)划分地层，查明隧道通过剖面的各地层深度与厚度。(2)查明地质构造，隧道轴线段有无断层、破碎带、软弱层面等。(3)查明基岩风化程度，从而结合速度资料进行围岩分级。(4)划分洞口地层结构，判断洞口稳定性。(5)查明地下水赋存情况及其活动动态。(6)其他不良地质现象存在情况。针对上述需要查明的地质问题与计算围岩分级情况，论文提出合理选用物探方法的原则，并统计了物性参数值在不同岩性中的分布范围。得出以下研究成果：(1)总结出各物探方法在隧道地质调查中的作用和优缺点。(2)得出不同地质条件下和不同勘察阶段，隧道工程物探方法选择和布置的原则。(3)根据以往工作经验和事例，对常用的物性参数进行了归纳总结，同时介绍了不同地质条件下物探方法选择的依据。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

与tunnel effect相关的网络解释 [注：此内容来源于网络，仅供参考]

subway tunne; underground railway tunnel：地铁隧道

水下隧道;水地隧道 subaqueous tunnel; underwater tunnel | 地铁隧道 subway tunne; underground railway tunnel | 浅埋隧道 shallow tunnel; shallow-depth tunnel; shallow burying tunnel

subway tunnel;underground railway tunnel;metro tunnel：地铁隧道

1642 公路隧道 highway tunnel | 1643 地铁隧道 subway tunnel;underground railway tunnel;metro tunnel | 1644 人行隧道 pedestrian tunnel

subaqueous tunnel：水下隧道

1646 山岭隧道 mountain tunnel | 1647 水下隧道 subaqueous tunnel | 1648 海底隧道 ;水下隧道 submarine tunnel;underwater tunnel