triumphant strains
- triumphant strains的基本解释
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凯, 恺
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The cultural condition of PGPR strains Temperature,pH,light,cultural method,acid or alkali producing,and salt tolerance of 9 PGPR strains isolated from Gramineous Forage were tested,the results showed that all the PGPR strains could grow in the temperature range from 5℃~45℃,and optimum temperature for 178,O-6,Dry6 strains was 35℃,for X5,173,Y5,C-6 strains was 30℃,for N4 strain was 25℃~35℃,for 86 strain was 25℃;most of PGPR strains prefered to neutral or alkaline condition,strains 178,O-6,N4 and X5 were preferable to alkali condition especially;light was beneficial to PGPR's growth;all of them produce alkali;most of PGPR strains were not sensitive to NaCl concentration;all the strains were aerobiotic bacteria.
结果表明:各供试菌株对温度的适应范围较广,在5℃~45℃范围内均能生长,178、O-6、Dry6 菌株的适宜生长温度为35℃,X5、173、Y5、C-6 菌株的适宜生长温度为30℃,N4 菌株的适宜生长温度为25℃~35℃,86 菌株的适宜生长温度为25℃;大部分菌株在中性或偏碱性的条件下生长好,特别是Dry6、N4 和X5 菌株对碱性环境适应性强,在pH 值8.0 时生长最好;光照有利于菌株的生长;绝大部分菌株在3%NaCl浓度下生长良好,在5%~7% NaCl 浓度下除173 和86 菌株外,其它菌株都能生长,即对盐份的耐受性较好,且9 个菌株均为产碱菌;除173 菌株是兼性厌氧细菌外,其它都是好氧性细菌。
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A series PCR amplification for differential control strains and DNA samples diluted gradient (1:10) have been used to evaluate the specificity and sensitivity of PCR assay established.Results 1. Detection of GAS by PCR assay: The 345bp specific fragment of speB gene were amplified in all the tested GAS strains including three strains of scarlet fever, whereas it was detected in none of the differential control strains. The lowest limit of detection was 6.5pg genome DNA of GAS strain. 2. Detection of corynebacterium diphtheria by PCR assay: The318bp specific fragment of toxB gene were amplified in all the tested toxigenic corynebacterium diphtheria strains, whereas it was detected in none of the differential control strains. The lowest limit of detection is 850fg/μl genome DNA of corynebacterium diphtheria strain. 3. Detection of Lp by PCR assay: The 340bp specific fragments of mip gene were amplified in all the tested Lp strains, whereas it was detected in none of the differential control strains including three strains of non-pneumophila.
结果:1、用PCR方法检测A组链球菌:以A组链球菌致热性外毒素基因speB为靶序列,设计的扩增引物对全部对照菌株的扩增结果为阴性,而全部A组链球菌参考株均能扩增出特异的345bp片段,其中包括三株猩红热链球菌,检测敏感性为6.5pg/μl DNA.2、用PCR方法检测白喉杆菌:以白喉外毒素基因toxB为靶序列,设计的扩增引物对全部白喉杆菌参考株均能扩增出特异的318bp片段,而全部对照株的扩增结果为阴性,检测敏感性为850fg/μl DNA.3、用PCR方法检测嗜肺军团菌:以嗜肺军团菌巨噬细胞感染增强子基因mip为靶基因,设计的引物对嗜肺军团菌14个血清型参考株均扩增出特异的340bp片段,而鉴别对照株包括三株非嗜肺军团菌均未扩增出任何片段。
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Methods One hundred and fifty strains of Malassezia yeasts from pityriasis versicolor (29 strains), Malassezia folliculitis (16 strains), seborrheic dermatitis (49 strains), onychomycosis (33 strains), and healthy individuals(23 strains) were studied, according to physiological and morphological features in culture, and compared to standard strains.
方法以标准株作对照,用生理生化学及形态学方法将150株来源于花斑癣(29株)、马拉色菌毛囊炎(16株)、脂溢性皮炎(49株)、甲真菌病(33株)及正常人皮肤(23株)的马拉色菌进行分类及描述,并分析了各菌种在一些皮肤病的分布情况。