transfer RNA

transfer RNA的基本解释

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转移核糖核酸, 传递核糖核酸

与transfer RNA相关的网络例句 [注：此内容来源于网络，仅供参考]

Methods: One-step trizol had used to get the total RNA of the four animal brain, then PEGS000 to purify the small RNA from the total RNA, after the ligation ofpoly on the 3\' and the adaptor on the 5\', the oligo primer will be used to reverse translation the small RNA, and then used the specific primers to amplification the small RNA sequences. After the gel purition recovery, the small RNA will be ligated to the T-vector, and be transformed to the TOP 10 E. coli, at last, we picked up the masc. clones for sequencing.

通过Trizol一步法提取四种动物脑中的总RNA，接着用PEG纯化其中的小分子RNA，再经过poly连尾反应在小RNA的3'端连接上一段poly，然后在5'端连上adaptor，用Oligoprimer将连接好的小RNA进行反转录，用根据adaptor和Oligoprimer设计的引物来扩增小RNA，扩增后的小RNA进行胶回收后连接到T载体上，转化TOP10大肠杆菌后挑取阳性克隆进行测序，得到的序列再经过生物信息学的分析后确定是否为miRNA。

RNA interference represents a common biological phenomenon of double-stranded RNA mediated post-transcriptional gene silencing, which lies in altitude plants and animals. The mechanism of this process is described as that: the dsRNA in cells are cut into double-stranded small interfering RNA. By joining with an effector complex termed RISC (RNA-induced silencing complex), siRNA binds to the cellular RNA with homologous sequences, and contributes to the degradation of the corresponding mRNA, the inhibition of specific gene expression.

RNAi技术是双链RNA(double strand RNA，dsRNA)介导的序列特异性的转录后基因沉默，广泛存在于高等植物和动物中，其机制是细胞内的dsRNA被Dicer剪切成双链的小分子干涉RNA (small interfering RNA，siRNA)与RNA诱导的基因沉默复合物结合，降解与之互补的靶mRNA，特异性抑制靶基因表达。

The total RNA from brain tissue of substantia nigra of PD rats is extracted at the 1st, 2nd, 4th and 8th week and comparison is made between it and that from normal rats. The total RNA, after RNA formaldehyde degenerated sepharose electrophoresis and separation and Northern transfer, is adsorbed unto colloxylin membrane. Then ECL labeled Selenoprotein P and an unknown gene fragment are used as probes to hybridize with the RNA on the colloxylin membrane. And the hybridized result is obtained with enzyme-linked immunosorbent assay. Finally, analysis of OD is made using ONE-Dscan software.

分别提取1周、2周、1月、2月的帕金森大鼠（各8只）黑质区脑组织总RNA，并取相对应时间的正常大鼠的脑组织总RNA作为对照，经RNA甲醛变性琼脂糖凝胶电泳分离后，通过Northern转移，将其吸附在硝酸纤维素膜上，然后利用ECL标记的Selenoprotein P和一段未知基因为探针，与硝酸纤维素膜上的RNA分子进行杂交，再通过显影取得杂交的结果，应用ONE-Dscan软件进行光密度值分析。

与transfer RNA相关的网络解释 [注：此内容来源于网络，仅供参考]

transfer rna：转移

transfer potential 转移势 | transfer rna 转移 rna | transferable 传第性的

transfer rna：转移核糖核酸

transfer dna 转移 dna | transfer rna 转移核糖核酸 | transferase 移转酶

transfer rna：运输性

"输送泵","transfer pump" | "运输性RNA","transfer RNA; soluble RNA; tRNA" | "转移酶","transferase"

transfer rna：携带胺基酸到核 体上进 蛋白质合成的

83. ribosomal RNA: 含 最丰的RNA,构成核 体 | 84. messenger RNA: 携带遗传密码的RNA | 85. transfer RNA: 携带胺基酸到核 体上进 蛋白质合成的RNA

TRNA transfer RNA：转运核糖核酸

TRH thyrotropin releasing hormone 促甲状腺素释放激素 | tRNA transfer RNA 转运核糖核酸 | TSH thyroid stlmulating hormone 促甲状腺激素