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RESULTS The results indicated that PCF could significantly increase the viability of thymocytes and enhance the activities of SOD, GSH-Px, CAT in cells. PCF decreased the amounts of ROS and the rate of apoptosis in cells. PCF inhibited the morpholog alteration of the thymocytes irradiated by UVB and maintained the ultrastructure of cells in normal. PCF attenuated UVB caused DNA fragmentation in thymocytes. While combined ERKs inhibitor yielded typical DNA fragmentation, co-treatment of thymocytes with PCF and JNKs and p38 kinase inhibitors completely blocked UVB-induced DNA fragmentation.
结果 PCF能显著提高UVB辐射后小鼠胸腺淋巴细胞的活性;提高胞浆中SOD、GSH-px、CAT的活性,降低ROS含量及细胞凋亡率;抑制UVB辐射所致的胸腺淋巴细胞凋亡的形态学改变,维持细胞的正常超微结构;抑制UVB所致的胸腺淋巴细胞DNA片段的出现;预先应用ERK抑制剂使DNA片段增加,给予JNK和P38抑制剂后协同PCF使DNA片段带减少。
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We found that intravenously administered arginine significantly decreased the spleen weight, the serum interleukin-6 level, and IL-6 production by leukocytes, splenic macrophages, and thymocytes; significantly decreased tumor-necrosis factor-α production of leukocytes and thymocytes; and significantly increased the stimulating index of cell proliferation in T-thymocytes in peritonitic rats.
结果显示,静脉给予arginine显著降低腹膜炎大鼠脾脏重量、血清间白素-6、与白血球、脾脏巨噬细胞及胸腺细胞IL-6分泌量,且显著降低白血球与胸腺细胞肿瘤坏死因子-α分泌量,但显著增加胸腺细胞增生的刺激指数。
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To study the protective effects of polypeptide from Chlamys farreri on thymocytes damaged by H_2O_2 and antioxidative mechanisms of PCF,we made a thymocytes damaged-H_2O_2 model.
在有氧代谢过程中,机体会产生少量的活性氧,这些ROS可以被机体内存在的抗氧化酶和非酶抗氧化剂所清除。
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murine thymocytes:小鼠胸腺
肝癌细胞:hepatoma cell lines | 小鼠胸腺:murine thymocytes | 小鼠骨髓:Murine bone marrow