three character primer

three character primer的基本解释

n.

三字经

与three character primer相关的网络例句 [注：此内容来源于网络，仅供参考]

The method is just like the QuikChange site-directed mutagenesis method except for its special primer. This pair of primers is composed of a long primer and a short primer which is central overlapped.Both of the Tm of 5\'sequence in long primer and Tm of 3\'sequence in short primer are above 66℃.The Tm of short primer is almost half of that of long primer.It is convenient to perform insertion or substitution mutation of DNA fragments larger than 20bp,In addition,it is highly applicable in research about the deletion mutation of DNA fragments larger than 2000bp.

我们实验室一直在寻找一种简单高效的可以用于基因的删除、插入及替代突变的方法，通过大量研究和实验发现，可以使用一种基于反向PCR原理，使用全新的引物设计的突变方法——&COP&突变法，该方法采用与QuikChange点突变法相同的操作步骤，其策略是设计一对中心重合的引物，长引物的5\'端序列和3\'端序列的Tm值均不低于66℃，短引物Tm值约为长引物一半，且与长引物中心重合互补。

The invention discloses a method to analyze fish genetic information with mitochondria DNA D-loop controlling zone, which comprises the following steps:(1) extracting genom DNA and mitochondria DNA of fish;(2) designing simple primer in fish mitochondria DNA D-loop controlling zone; possessing twenty one basic groups CAC CCY TRR CTC CCA AAG CYA in upstream primer MitD1-F; possessing twenty three basic groups GGT GCG GRK ACT TGC ATG TRT AA in downstream primer MitD1-R;(3) proceeding PCR reaction under the function of primer;(4) choosing agarose jel electrophoresis;(5) checking sequence for augment mtDNA D-loop gene fragment.

本发明公开了一种利用线粒体DNA D-loop控制区进行鱼类遗传信息分析的方法。它包括如下步骤：(1)分别提取鱼类基因组DNA和线粒体DNA；(2)在鱼类线粒体DNA D-loop控制区设计简并引物：其上游引物MitD1-F有21个碱基：CAC CCY TRR CTC CCA AAG CYA；下游引物MitD1-R有23个碱基：GGT GCG GRK ACT TGC ATG TRT AA；(3)在引物的作用下进行PCR反应；(4)琼脂糖凝胶电泳；(5)对扩增的mtDNA D-loop基因片断进行测序。

In these portion,the article begins the study of tone sandhi from the point view of two-character phrases and three-character phrases,which is also the focus and difficult point as well.On the basis of substantial materials of Dingzhou dialect,the author elaborates its rules and principles of tone sandhi of the general phrases and those with light tones,and reiterative location.the author puts his views as follows: 1.The rules of tone sandhi of two- character phrase are simple and unitive. 2.The complex of the phrase with light tones lies in the combination of the first tone and light tone.In the case,there are two different pronunciations in Dingzhou dialect.The author guesses it is the coexist of ancient pronunciations and common spoken Chinese. 3.The tone sandhi of general three-character phrases on the basis of the rules of that of two- character phrases,using the grammar structure of the three-character phrases as the restricted conduction,which function jointly. 4.The rules of tone sandhi of three- character phrases with light tones are restricted only by that of the two- character phrases with light tones and thus are foreign to grammar structure. 5.The tone sandhi of the words with reiterative location has their own system.

这两章中，作者采用直接调查法，社会语言学方法分别从一般字组、含轻声字组和叠音词字组三个方面对定州方言连读变调进行详尽地描写，文章中既有对连读变调的共时性描写，也有对连读变调历时性推测，总的来讲笔者认为定州方言变调具有一定的系统性，主要表现在以下几个方面：两字组连读变调规律简单统一，含轻声字两字组内部规律较为复杂，但是具有很强的一致性；定州方言含轻声两字组变调的复杂性主要表现在阴平+轻声的组合中，在这种情况下，定州方言有两种读音方式，推测它是语音存古现象和普通话语音并存的结果；一般三字组连读变调以两字组连读变调规律为基础，以三字组的语法结构为制约条件，二者共同发生作用；轻声三字组变调规律只受轻声两字组变调规律的制约，与语法结构无关；叠音词的变调规律自成系统。

与three character primer相关的网络解释 [注：此内容来源于网络，仅供参考]

primer detonator：起爆雷管

primer charge 点火药 | primer detonator 起爆雷管 | primer 引爆药包

nitrocellulose primer：硝基底漆

末道底漆 finish primer | 硝基底漆 nitrocellulose primer | 磷化底漆 etch primer

nitrocellulose primer：磷化底漆

finish primer 硝基底漆 E~Uqn!bt | nitrocellulose primer 磷化底漆 K]!w | etch primer 硝基面漆 <'J]?B- (q