tam-o'-shanter [tæmə'ʃæntə]
- tam-o'-shanter的基本解释
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n.
大黑头巾形帽子
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To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.
1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry,FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时,对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后,Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR,RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时,Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope,LSM)观察北豆根总碱(0、40μg/ml)作用24小时后,Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。
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Patrick Tam is better known today as Wong Kar-wai's mentor (Wong worked under Tam's wing as his scriptwriter on the 1987 Final Victory; and Tam edited Days of Being Wild and Ashes of Time) but he will be remembered for Nomad, one of the youth movies of the 80s that marry libertarian impulses with those impulses of social responsibility that somehow makes this an oxymoronic youth movie -- being heavy in the social and light in the libertarian.
谭家明,多数人都知道他是王家卫的导师(1987年谭家明拍《最后胜利》时,王家卫是他的编剧,谭家明也负责阿飞正传和东邪西毒的剪辑),但他的《烈火青春》,一部80年代年轻人在自由主义与社会责任之间迷惑矛盾的电影,应该永留史册。
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Also ducks. I remember that they were most peculiar ducks with top-knots that looked like tam-o'-shanter caps.
到那里去的还有鸭子,我觉得他们的顶冠最奇特了,就像苏格兰人戴的宽边圆帽似的。
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tam-o'-shanter:大黑头巾形帽子
static shift 静态偏移 | tam-o'-shanter 大黑头巾形帽子 | nucleoliform 核仁形
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tam-o'-shanter:头巾形帽子
tam 便帽 | tam-o'-shanter 头巾形帽子 | tam-tam 铜锣
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tam-o'-shanter:苏格兰圆扁帽
仿针织精纺呢 tammy | 苏格兰圆扁帽 tam-o-shanter | 止血棉带 tampon tape
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tam-o'-shanter:<农夫汤姆>
'The Jolly Beggars' | 'Tam o'Shanter' | Kilmarnock edition 基尔马诺本