tadpole-shaped
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The left lens of Anuran Tadpole - Bufo Raddei Strauch Tadpole and Xenopus laevis Tadpole were removed when they had posterior limb bud. By decapitating and fixing it with Carnoy's fixation fluid in different days, by means of paraffin serial section and Hematoxylin-Eosin stain and by using the microscope to observe the microstructure of lens regeneration of Bufo Raddei Strauch Tadpole and Xenopus laevis Tadpole. By extirpating the left eye and fixing it with aldehyde and osmium acid , by using electron microscope to observe the ultrastructure of lens regeneration of Bufo Raddei Strauch Tadpole and Xenopus laevis Tadpole.
以无尾两栖类—以花背蟾蜍与非洲爪蟾后肢芽期蝌蚪为实验材料,摘除其左眼晶状体,在再生不同时期固定,石蜡连续切片,苏木精-伊红对染,光镜观察,研究晶状体再生的组织来源;另外,选再生不同时期材料用戊二醛,锇酸固定,透射电镜观察,以研究晶状体再生组织的超微结构变化。
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Xenopus laevis Tadpole (1) Xenopus laevis Tadpole was capable of lens regeneration, which originated from the outer cornea.(2)The days of lens regeneration of Xenopus laevis Tadpole was earlier than Bufo Raddei Strauch Tadpole, The rate of regeneration of Xenopus laevis Tadpole was higher than Bufo Raddei Strauch Tadpole.(3)During Xenopus laevis lens regeneration the outer cornea cells proliferated, in the elongating cells the mitochondria became complicated and in the columnar cells the cisternal dilating of endoplasmic reticulum was ubiquity.
非洲爪蟾蝌蚪(1)再生晶状体来源于外角膜;(2)晶状体再生较花背蟾蜍蝌蚪晶状体再生时期早,再生率高;(3)电镜观察发现再生前期外角膜增殖,观察到晶状体上皮细胞和伸长细胞(进一步发育为晶状体纤维细胞),伸长细胞中线粒体形状各异随着进一步的分化,细胞器逐渐消失;分化过程中晶状体上皮细胞粗面内质网增多,并且内质网扩张普遍存在。
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CGRP content in myocardium gradually enhanced from normal group to controls group, and model group's was the highest. We found immunopositive neurons and nerve fibers of nNOS and CGRP by immunochemistry in normal myocardium. They were mainly round-shaped and oval-shaped, also tadpole-shaped、 shuttle-shaped、triangle-shaped、ring-shaped and irregular-shaped. Nv of neurons and nerve fibers in atria were more than in ventricle in some degree; Those in right part of heart were more than in left. Morphology of CGRP neurons changed in model group, the number of ring-shaped neurons increasing, while the round-shaped and oval-shaped decreasing, and Nv and Sv of model group were markedly less than normal. Expression of nNOS mRNA was overly higher than normal group, but CGRP was opposite.
心肌组织CGRP的含量由正常组、实验对照组至CHF组逐渐升高,差异显著;免疫组化研究表明心肌组织中存在nNOS和CGRP免疫阳性神经细胞及纤维,细胞形态多样,正常多以圆形和椭圆形为主,还有梭形、蝌蚪形、三角形、指环形及不规则形,细胞密度心房高于心室,右心高于左心;CHF组CGRP神经细胞形态有一定改变,淡染的指环形细胞增多,而深染圆形或椭圆形细胞减少,其面、数密度均明显低于正常组,差异显著;用RT-PCR测得CHF组nNOS mRNA极度表达,显著高于正常组,而CGRP表达明显低于正常组。
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tadpole nests:蝌蚪穴
tadpole diagram 蝌蚪图 | tadpole nests 蝌蚪穴 | tadpole plots 蝌蚪图
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tadpole diagram:蝌蚪图
tactron 冷阴极充气管 | tadpole diagram 蝌蚪图 | tadpole nests 蝌蚪穴
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tadpole plots:蝌蚪图
tadpole nests 蝌蚪穴 | tadpole plots 蝌蚪图 | Taeniaesporites 四肋粉属