tadpole fish
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The left lens of Anuran Tadpole - Bufo Raddei Strauch Tadpole and Xenopus laevis Tadpole were removed when they had posterior limb bud. By decapitating and fixing it with Carnoy's fixation fluid in different days, by means of paraffin serial section and Hematoxylin-Eosin stain and by using the microscope to observe the microstructure of lens regeneration of Bufo Raddei Strauch Tadpole and Xenopus laevis Tadpole. By extirpating the left eye and fixing it with aldehyde and osmium acid , by using electron microscope to observe the ultrastructure of lens regeneration of Bufo Raddei Strauch Tadpole and Xenopus laevis Tadpole.
以无尾两栖类—以花背蟾蜍与非洲爪蟾后肢芽期蝌蚪为实验材料,摘除其左眼晶状体,在再生不同时期固定,石蜡连续切片,苏木精-伊红对染,光镜观察,研究晶状体再生的组织来源;另外,选再生不同时期材料用戊二醛,锇酸固定,透射电镜观察,以研究晶状体再生组织的超微结构变化。
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Xenopus laevis Tadpole (1) Xenopus laevis Tadpole was capable of lens regeneration, which originated from the outer cornea.(2)The days of lens regeneration of Xenopus laevis Tadpole was earlier than Bufo Raddei Strauch Tadpole, The rate of regeneration of Xenopus laevis Tadpole was higher than Bufo Raddei Strauch Tadpole.(3)During Xenopus laevis lens regeneration the outer cornea cells proliferated, in the elongating cells the mitochondria became complicated and in the columnar cells the cisternal dilating of endoplasmic reticulum was ubiquity.
非洲爪蟾蝌蚪(1)再生晶状体来源于外角膜;(2)晶状体再生较花背蟾蜍蝌蚪晶状体再生时期早,再生率高;(3)电镜观察发现再生前期外角膜增殖,观察到晶状体上皮细胞和伸长细胞(进一步发育为晶状体纤维细胞),伸长细胞中线粒体形状各异随着进一步的分化,细胞器逐渐消失;分化过程中晶状体上皮细胞粗面内质网增多,并且内质网扩张普遍存在。
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We got the result: Bufo Raddei Strauch Tadpole (1) Bufo Raddei Strauch Tadpole was capable of lens regeneration, which originated from the epithelial cells at iris dorsal margin by dipigmentation.(2) Dipigmentation of Bufo Raddei Strauch by two methods: one was licked up by amoebocyte, the other one was flowed from the pigment cells.(3) In the lens regeneration cells of Bufo Raddei Strauch profiles of the mitochondria became complicated ribosome manifold, and the endoplasmic reticulum was cisternal dilation. During the process of deferentiation of lens fiber the cell nucleolus became little and chromatin shrinked.(4)The crystalline electrophoresis of Bufo Raddei Strauch Tadpole lens regeneration showed that during lens regeneration P-crystalline was expressed earliest then was γ-crystalline and α-crystallin, this result is consistent with newt.
结果表明:花背蟾蜍蝌蚪(1)具有晶状体再生的能力,其晶状体的再生来源于虹膜背缘的上皮细胞;(2)虹膜背缘去色素是转分化的前提,主要有两种方式:一种也是最主要的方式是在晶状体被摘除后虹膜外侧产生巨噬细胞,巨噬细胞吞噬虹膜色素上皮细胞中的色素颗粒;另一种方式是虹膜色素上皮细胞自身释放色素颗粒;(3)电镜观察发现虹膜色素上皮细胞转分化过程中线粒体有明显变化,核糖体增多,粗面内质网增多,晶状体纤维分化过程中细胞核逐渐凝聚;(4)SDS-PAGE电泳发现,花背蟾蜍蝌蚪晶状体再生中相关蛋白表达顺序与有尾类蝾螈相似,蛋白表达顺序依次是β-晶状体蛋白、γ-晶状体蛋白和α-晶状体蛋白,并且后两种蛋白几乎同时表达。
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tadpole fish:狗鳕
tactic polymer 有规聚合物;立体异构聚合物 | tadpole fish 狗鳕 | Taenia 絛虫属