tRNA
- tRNA的基本解释
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(=transfer RNA)转移RNA
- 相似词
- 更多 网络例句 与tRNA相关的网络例句 [注：此内容来源于网络，仅供参考]
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Recombinant ptotein made up 39.2%(pET-20b-bglA) and 33.5%(pET-28a-bglA) of the total proteins in the intracellular fraction, the solubility proportion of the enzyme up to 32.8%(pET-20b-bglA) and 40.1%(pET-28a-bglA), the activities of the enzyme were 66.8 (pET-20b-bglA) and 71.2 U/mg (pET-28a-bglA). These results showed that E. coli BL21-CodonPlus (DE3)-RIL with argU tRNA, ileY tRNA and leuW tRNA genes helped to improve expression of the enzyme through enhanced identification of the rare codons AGA/AGG, AUA and CUA. Further optimized the conditions for inducing, the solubility proportion of the enzyme was 70.6% at 16 °C and 1.2 times higher than 37 °C. The solubility proportion of the enzyme increased from 12.3% to 32.8% when IPTG concentrations dropped from 1000 μM to 25 μM. The recombinant enzyme was purified by heat treatment, DEAE Sepharose anion-exchange chromatography and TOYOPEARL HW-55F.
maritima MSB8 的-葡萄糖苷酶基因 bglA克隆至表达载体 pET-20b 和 pET-28a，构建重组质粒 pET-20b-bglA 和 pET-28a-bglA，然后转化不同大肠杆菌 E.coli 宿主，Tm-SIGlA 在 E.coli BL21-CodonPlus(DE3)-RIL 中获得高效表达，重组蛋白的表达量为 33.5%(pET-28a-bglA)和 39.2%(pET-20b-bglA)，可溶性比例为 32.8%(pET-20b-bglA)和 40.1%(pET-28a-bglA)，比酶活达 66.8 (pET-20b-bglA)和 71.2 U/mg (pET-28a-bglA)，这些结果表明，E.coli BL21-CodonPlus(DE3)-RIL 宿主带有的 argU tRNA、ileY tRNA 和 leuW tRNA 基因，分别增强对稀有密码子 AGA/AGG、AUA 和 CUA 的识别，有助于提高该酶在 E.coli 中的表达；进一步优化诱导条件，重组 E.coli BL21-CodonPlus(DE3)-RIL/pET-20b-bglA 在 37 ℃下诱导培养，IPTG 浓度由 1000 μM 降至 25 μM，目的蛋白可溶性表达由 12.3%增至 32.8%，提高 1.7 倍，16 ℃下低温诱导实现目的蛋白 70.6%的可溶性表达，较 37 ℃下诱导培养提高 1.2 倍。
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By the methods of molecular biology, enzymology, chemistry, physics the project was carried out. The genes encoding leucyl- and arginyl-tRNA synthetase , and tRNA and tRNA from Escherichia coli were cloned and overexpressed in E.
以大肠杆菌亮氨酰tRNA合成酶和精氨酰tRNA合成酶为对象用分子生物学、酶学、酶动力学、化学、物理学的方法进行该项研究。
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Four types of start codons were identified in the 13 protein coding genes, in which TAA and TAG were used in 12 PCGs as stop codons, except ND5, Most tRNA genes could form typical secondary structures except tRNA, which had an absence of the DHU arm.
除tRNA外，其余21个tRNA的二级结构均属典型的三叶草结构。tRNA的DHU臂缺失，在相应的位置上只形成一个环。
- 更多网络解释 与tRNA相关的网络解释 [注：此内容来源于网络，仅供参考]
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peptidyl transferase：基转移酶
或者两者紧密相接.进入的 tRNA 分子在它所带的氨基酸形成肽键后,就从 A 位置移到 P 位置.详细地说,带有某一氨基酸的 tRNA 分子进入 A 位置(图 11-38⑤),然 后由于肽基转移酶(peptidyl transferase)的作用,它所带的氨基酸跟 P 位置上 tRNA 所 带的氨基酸形成肽键,
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acceptor site：受位
(一)转肽酶以及给位与受位 核糖体相当于"装配机"能够促进 tRNA 所带的氨基酸缩合成肽.核糖体有 2 个位置分 别称为给位(donor site)与受位(acceptor site) ,供携带氨基酸或新生肽链的 tRNA 附着.给 位又称为 P 位(peptidyl site,肽位) ;
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stringent control：应急控制[细菌子 氨基酸饥饿时发生的rDNA、tRNA 基因及核糖体蛋白基
streptothricin 链丝菌素 | stringent control 应急控制[细菌子氨基酸饥饿时发生的rDNA、tRNA 基因及核糖体蛋白基 | subcellular 亚细胞的