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METHODS: Neonatal mouse brain tissues were sterilely obtained to prepare monoplast suspension.
无菌条件下取新生小鼠脑组织,制备单细胞悬液。
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METHODS: Fetal liver was obtained sterilely. Monoplast suspension was collected by collagenase digestion and mechanical separation, and then centrifuged using 1.070 g/mL Percoll separating medium and 1.077 g/mL Ficoll separating medium. Cells achieved from the interface of separatory liquids were cultured in DMEM/F12 medium, supplemented with 0.1 volume fraction of fetal bovine serum, 20 μg/L hepatocyte growth factor and 40 μg/L epidermal growth factor, for 9 days.
无菌状态下取出胎儿肝脏,以胶原酶消化法和机械分离法获得胎儿肝脏来源单细胞悬液,分别采用1.070 g/mL Percoll分离液和1.077 g/mL Ficoll分离液进行密度梯度离心,吸取界层细胞,添加含体积分数为0.1 FBS、20 μg/L肝细胞生长因子、40 μg/L表皮生长因子的DMEM/F12新鲜培养基诱导9 d。
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ML of bone marrow was sterilely aspirated from the posterosuperior iliac spine of 36 healthy mongrel dogs. BMSCs were isolated and purified by Percoll density gradient centrifugation and cultured in vitro by adherence method.
选择健康杂种犬36只,无菌抽取犬骨髓液10 mL,经Percoll液密度梯度离心后贴壁法体外培养骨髓间充质干细胞,传2代后行5-氮胞苷诱导,5-溴脱氧尿苷标记备用。
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All patients received a massive bone allograft that had been sterilely procured without secondary irradiation.
所有的病人都接受了经消毒而未进行二次辐射的大块同种异体骨。
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METHODS: Bone marrow was sterilely isolated. BMSCs were isolated and purified by the density gradient centrifugation.
无菌条件下抽取猪骨髓液,密度梯度离心法分离纯化骨髓间充质干细胞。
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