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The multi-copy expression vector pZM is a shuttle plasmid which can replicate in Escherichia coli, Streptomyces and S. erythraea. It contains PermE promoter, fd terminator, multiple clone site, thiostrepton and ampicillin resistance genes, and ColE1 ori and pJV1 ori replicons which make the vector replicate in E.
游离型多拷贝表达载体pZM是可以在大肠杆菌、链霉菌和糖多孢红霉菌中扩增的穿梭型质粒,带有PermE启动子、fd终止子、多克隆位点、硫链丝菌肽和氨苄青霉素抗性基因以及在大肠杆菌和糖多孢红霉菌中复制的ColE1 ori和pJV1 ori复制子,载体大小9880bp。
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The replicons were detected and characterized by RT-FUR. IFA. Renilla Luciferase assay system and real time RT-FUR. respectively. Results It was shown that SLB2 mutant did out significantly affect the translation of the input RNA.
将突变体(包括相关的阳性和阴性对照复制子)分别线性化并体外转录成RNA后,取等量各转录体RNA,以脂质体法分别转染BHK-21细胞。
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This result was also confirmed by neomycin gene-containing replicons with same mutations.
这一结果在新霉素包含子转染中也得到了证实。
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All replicons were down-regulated by ribavirin, but the level of reduction in the V85I mutant was the lowest.
所有复制子均受病毒唑的调节,但是在 V85I 突变异种中水平最低。
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All replicons were down-regulated by ribavirin, but the level of reduction in the V85I mutant was the lowest.
这一结果也被包含相同突变的新霉素基因复制子的结果证实。
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