regular quantity
- regular quantity的基本解释
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正则元素
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To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.
为在毕赤酵母中分泌表达人肝再生增强因子,以色谱法分离纯化后进行体外活性研究,构建表达载体pPICZαA- ALR,经电穿孔转入毕赤酵母中,用0.5%甲醇诱导表达;重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明, rhALR以分子量为30kD的二聚体为主;定量分析结果表明,重组酵母培养上清中rhALR约占总蛋白的66%,表达量约为40mg/L;经DEAE柱和G75柱纯化后,获得的rhALR纯度大于95%,得率为52%;体外生物学活性实验表明,rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。
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And flexibility of common weapons can not meet their requirement, they have no capacity of completing tasks of battles flexibly according to actual situation just like human being; furthermore, common weapons should be made in large quantity for completement of various tasks during terms of war because weapons just like arrows have only one life for use, thus they will consume extra materials and energy that may be consumed by people, so merely use these regular weapons is not economical; and Japan has small quantity of population which should not be wasted rashly.
第二,日本人口基数不大,资材不不丰富,但是完成扩大活动空间的愿望却很强烈,又一般的机器武器灵活性不够,不能象人那样具备随机应变的能力来完成战争任务;且因为象火箭这些机器武器的使用是一次性的,完成战时各项任务又需要大量制造,这会消耗掉本应由人消耗的物质能源,仅用这些武器是不划算的;而日本人口基数不高,人是耗不起的。
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The mount of germ and fungus quantity in soil are remarkably increased for the continuous cropping about 5 years, puts the trichobacteria quantity always change tendency to reduce;The edaphon total quantity, the bacterium quantity change reduces generally above the continuous cropping 5 years and below 5 years (including 5 years) the change are not obvious;In the nitrogen physiology group by ammoniates the bacterium primarily and the quantity changes are not very big;The nitrifying bacteria quantity is lower than the comparison (non- continuous cropping), the quantity of denitrifying bacteria is opposite;The alkalinity phosphatase, the urease, invertin activeness have a closed relations with the continuous cropping when it will increase less than 5 years or decrease in more than 5 years. The relation between catalase activeness and the continuous cropping is not very big. The reduction of the invertin activeness, the alkalinity phosphatase, urease activeness have slightly elevates, catalase activeness have no the regular changes.
表现为连作条件下土壤真菌数量显著增大,放线菌数量总的变化趋势降低;土壤微生物总量、细菌数量变化在连作5年以上普遍降低,5年以下(包括5年)则变化差异不明显;氮素生理群中以氨化细菌为主,但数量变化不大;硝化细菌数量低于对照组,反硝化细菌数量则相反:多酚氧化酶、转化酶活性在5年之前逐渐上升,5年之后逐渐下降,,脲酶活性随着连作年限增加持续上升,过氧化氢酶活性、碱性磷酸酶活性变化无规律。
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devastate: destroy completely:破坏
11.deplete: lessen greatly in quantity,contents,power or value 使减少,使枯竭 | 12.devastate: destroy completely 破坏 | 13.rotate: to plant different plants each year; take turns in regular order 轮作