puparial
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Apart from body color of puparial cuticle, there were microstructure differences which were found out by scanning electron microscopy. SEM had denser, more packed structure of the puparial exuviae than that of SEW.
扫描电镜分析结果表明,相对于SEW,SEM的蛹表皮结构更加的致密,表明黑化除了引起蛹的体色发生变异外,还引起蛹表皮结构发生了细微的差异。
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In order to identify the mechanism of melanic mutant of S. exigua, genetic regulation on pupae body color, puparial exuviae, phenoloxidase activity, dopamine andβ-alanine of SEW and SEM were investigated.
本文以SEW和SEM为材料,分别从蛹黑色性状的遗传规律、多酚氧化酶活力、电镜分析、高效液相分析、多巴胺和β-丙氨酸的微量注射及饲喂诱变几个方面进行了研究,以期找出甜菜夜蛾黑化的机理。
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Injection of 0.06mg had no effect on the puparial color of SEM, and injection of 0.06 to 0.22mg ofβ-alanine gave an intermediate phenotype.
注射试验证明,SEM蛹的黑化可能是由于多巴胺的增多或β-丙氨酸的减少引起的。
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Whenβ-alanine (0.22mg/insect) was injected into pupae of SEM, the resulting puparial phenotype was similar to that of SEW.
将多巴胺和β-丙氨酸同时注射入试虫时,蛹的颜色变化不明显。
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The phenotype was partially maintained when β-alanine was co-injected with dopamine. The results indicated that higher level of dopamine, or lower level ofβ-alanine, caused the black puparial cuticle of SEM.
用含有多巴胺和β-丙氨酸的饲料分别饲养SEW和SEM,也可以诱导蛹的体色发生转变,但改变的只是表现型,这种表现型不能稳定遗传。