plasmid ['plæzmid]
- plasmid的基本解释
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n.
[生]质粒, 质体
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The omp17.3 gene of Brucella abortus was amplified by PCR and then the amplicon was cloned into the eukaryotic expression plasmid pcDNA3.1 to construct a recombinant plasmid pcDNA3.1-omp17.3. Then the recombinant plasmid pcDNA3.1-omp17.3 was transfected into COS7 cells, and the expressed OMP 17.3 was detected by Western-blotting.
采用PCR方法扩增了布鲁氏菌17.3ku外膜蛋白编码基因,并将该基因克隆至真核表达载体pcDNA3.1中,成功构建了真核表达质粒pcDNA3.1-omp17.3.pcDNA3.1-omp17.3转染COS-7细胞后,通过Western-blotting检测到了17.3ku蛋白的瞬时表达。
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Result: 1.(1) Three strips come to appear in the position of 6.0kb, 5.4kb and 600bp after gelose electrophoresis and their size accord with recombined plasmid DNA, pcDNA3 plasmid and VEGF165 gene accordingly.(2) Pure degree of the pcDNA3-VEGF165 recombined plasmid is: OD260/OD280=1.87. Its
在进行了重组质粒的扩增、提取、纯化、鉴定、兔MSCs的原代及传代培养以及RT-PCR、Western Blot 转染检测后,实验取得了令人满意的结果,证实了MSCs 能在体外有效转录外源VEGF 基因并分泌出VEGF 蛋白,同时也说明pcDNA3-VEGF165重组真核表达质粒是有效的。
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At the same time, intact shuttle-plasmid pZ189 was also introduced into the host cell which was used for detection of nontargeted mutation frequency. After replicating in cells, progeny plasmid DNA was isolated and transformed into E coli. MBM7070.In the presence of ampicillin, X-gal and IPTG, pZ189-transformed E. Coli MBM7070 forms blue colony if the plasmid contains an active SupF suppresser tRNA gene, and white or light blue colony if the SupF tRNA gene is inactive.
但是由于检测非定标性突变需同时引入穿梭质粒pZ189在细胞中复制,并回收质粒DNA,通过转化宿主菌E.coliMBM7070,在含x-gal和IPTG的氨苄指示板上培养,如果用于检测突变的靶基因SupF tRNA基因发生突变,则菌落色泽为白色或淡蓝色,而正常者为兰色。
- 更多网络解释 与plasmid相关的网络解释 [注:此内容来源于网络,仅供参考]
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plasmid incompatibility:质粒不相容性
研究人员的新方法利用了一种叫做质粒不相容性(plasmid incompatibility)的自然过程--当一种新的质粒进入已经含有质粒的细胞中时,两种质粒就会竞争资源,而竞争的结果就是一个胜利另一个则被消除.
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plasmid incompatibility:质粒不相容性[在无选择压力的条件下同一不相容群的质粒不能共存于同一细胞]
plasmid 质粒 | plasmid incompatibility 质粒不相容性[在无选择压力的条件下同一不相容群的质粒不能共存于同一细胞] | plasmin (血)纤(蛋白)溶酶
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plasmid incompatibility:质粒不亲和性
质粒 dna plasmid dna | 质粒不亲和性 plasmid incompatibility | 质粒转导 plasmid transduction
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plasmid incompatibility:质粒不相容性[在无选择压力的条件下同一不相容群的质粒不能共存
plasmid|质粒 | plasmid incompatibility|质粒不相容性[在无选择压力的条件下同一不相容群的质粒不能共存 | plasmin|(血)纤(蛋白)溶酶
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promiscuous plasmid:滥交质粒[可以在多种不同种属的细菌中自动传播的接合质粒]
prolinase 脯氨酰氨基酸二肽酶 | promiscuous plasmid 滥交质粒[可以在多种不同种属的细菌中自动传播的接合质粒] | promoter 启动子
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