plasma membranes
- plasma membranes的基本解释
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[医] 质膜
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- plasma membrane
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Subcellular localization of ATPase activity in the flesh of developing apple fruit was carried outusing a lead phosphate precipitation technique via electron-microscope. The ultrastrcture of phloemand its surrounding cells was also observed. The results showed that ATPase activity wasdominantly localized on the plasma membranes of sieve element/companion cell complex.
应用铅沉淀的细胞化学方法,研究了发育过程中苹果果肉内卸载区(韧皮部及其周围薄壁细胞)ATP酶的亚细胞分布和变化,并且,作为补充,观察了韧皮部及其周围薄壁细胞的超微结构。
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During cytokinesis, cortactin localized at Golgi-pools and the new plasma membranes between two daughter cells.
在胞质断裂时期cortactin定位于高尔基体和断裂处子细胞间细胞膜的位置。
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Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.
发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合,而且结合是饱和性的、可被竞争的,符合配体-受体结合的典型特征,同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性,表明水稻细胞质膜上存在nsLTP的受体;利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜,再进行结合实验,结合活性几乎不受影响;而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失,表明其受体为没有经过糖基化修饰的蛋白质;利用交联剂BS~3交联配体一受体后,再进行SDS-PAGE分离和放射自显影,结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合,可能是其受体;细胞外碱化实验表明,nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应,却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应,表明nsLTP和Cryptogein结合细胞膜上相同的位点,保护了植物细胞免受Cryptogein导致的细胞程序性死亡,并诱导系统获得性抗性的产生;体外Pull-down实验表明,nsLTP144和水稻的OsCaM1具有相互作用,暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。
- 更多网络解释与plasma membranes相关的网络解释 [注:此内容来源于网络,仅供参考]
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adipocyte plasma membranes:abbr. apm; 脂肪细胞膜免疫
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plasma membranes:质膜
质膜:Plasma membrane | 质膜:plasma membranes | 血糖:Plasma ammonia