phosphatese
- phosphatese的基本解释
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[医] 磷酸酯合成酶
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The contents of Riboncules acid, 5 -hydroxyptamine (5-HT), Cyclic adenosine monophosphate , Cyclic guanosine monophosphate in milk; and the activites of Alkine phosphatese , lactic dehydrogemase in serum were determined by Chromometry and radioimmunoassay.
采用比色法和放射性免疫法测定了西农莎能奶山羊乳中RNA,S-HT,CAMP,CGMP和血清中LDH,AKP的含量为:0.432,0.408,0.405,0.473,0.194,0.405。
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Osteoblasts were detected by alkaline phosphatese and calcium salt staining. The expression of osteopontin was measured by Western blotting.
成骨细胞鉴定采用组织化学方法行碱性磷酸酶和钙化斑块染色同时应用Western blotting法测定骨桥蛋白表达。
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The results indicated that the content of organic matter of the natural meadow and the arbor forest land in the 0-20 cm surface soil grew separately by 36.11% and 32.96%; the nitrogen content of the natural meadow and the arbor forest land was also high, being 66.88 mg/kg and 69.37 mg/kg, respectively; the content of fast-acting potassium in the surface soil was higher than that of meadow ,while the activity of the urea enzyme, alkaline phosphatese and sucrase of the arbor forest land was stronger than that of the other four land utilization modes.
结果表明,天然草地和乔木林地10~20 cm表层土壤有机质增长率均较大,为36.11%和32.96%,二者碱解氮含量也较高,分别为66.88和69.37mg/kg;乔木林地和灌木林地0~20 cm 表层土壤有效磷含量均高于天然草地和人工草地,乔木林地速效钾含量最高。
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The Charon library DNA was then digested with restriction enzymes BamH Ⅰ and Hind Ⅲ, and DNA fragments of length of about 0.5-1. 5kb was subcloned into plasmid pUC 18 which was also digested by BamH Ⅰ and Hind Ⅲ and dephosphorylated by alkaline phosphatese before being ligated with Charon fragments.
抽提λCharon文库总DNA,用BamHⅠ和HindⅢ完全酶切电泳,用低熔点琼脂糖回收长约0.5-1.5kb的DNA片段;连接到pUC18上,pUC DNA先用BamHⅠ和HindⅢ酶切,后用碱性磷酸酯酶脱磷。