pep talk

pep talk的基本解释

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鼓舞士气的讲话, 动员发言, 鼓励话, 勉励语

与pep talk相关的网络例句 [注：此内容来源于网络，仅供参考]

Results We obtained a lysogenic bacteriophage MZTP01with clear plaque and 1 mm diameter. Fragment D with 2362bp (Genebank No. AY639599) was obtained after the phage DNA hydrolyzed by HindⅢ/EcoRⅠ. Among fragment D, the pep gene with molecular weight of 47 kDa and length of 1101bp was cloned and expressed. Recombinant M15 (pQE30pep) was built and overexpressed in Escherichia coli with a 47kDa clear band. At the same place a clear band was observed by Western blot. Judging from the time course expression, we could conclude that PEP protein produced at 1 hour after induction and then increased gradually. PEP protein was mainly in the form of inclusion body in the recombinant and slowed the growth speed of host. Homologous comparison of PEP protein from phage MZTP01 with other PEPs from BLAST were that phage MZTP01 PEP protein had 100% homologe with that of Escherichia coli K12, and most of others took the similarity in the range between 37%~84%.

诱导获得的溶原性噬菌体MZTP01斑点清晰，直径约1mm，成斑时间12h；从噬菌体基因组DNA双酶切(HindⅢ/EcoRⅠ)片段中回收长度为2362bp的D片段(Genbank登录号： AY639599)，又从D片段中克隆了长度为1101bp、编码367aa、分子量为47kDa的pep基因，表达载体M15(pQE30pep)在大肠杆菌(Escherichia coli， E.coli)中表达获得了47kDa的清晰表达带，在1h 时开始产生蛋白并有逐步上升的趋势； Western blot 也在47kDa处得到一条清晰的条带；可溶性分析表明PEP蛋白在重组菌株中是以不可溶的包含体形式存在的，该蛋白的产生明显地抑制了宿主的生长速度；噬菌体PEP氨基酸序列之间的同源性比较表明，噬菌体MZTP01 PEP蛋白与来自E.coli K12噬菌体的PEP蛋白的同源性程度最大。

Like a shooting star Your headed by me You touched my heart But won't set me free Oh boy You've got to be patient with me Oh boy I wanna get to know you much better let's start it out Being friends for awhile You hold my eye When I first saw your smile Oh boy This could really turn into something Oh boy That's why I don't wanna rush it Don't talk Don't tell your friends About us Don't talk Or this all will end I promise Be quiet, be quiet Or I will just deny it Be quiet, ssshh Keep it between us I'm going out, with a bunch of my friends You can meet up with us at quarter to ten Oh boy Don't act like there's something between us Oh boy From now on, let's keep it a secret Don't talk Don't tell your friend About us Don't talk or this will end I promise Be quiet, be quiet Or I will just deny it Be quiet, sshh Keep it between us I know it feel that this is real But I need sometime to see If you're gonna be the one for me From now let's just keep it hush Let's keep it between us This is the real reason I don't really care Cuz people likes to talk, people like to stare Just one thing I guess, just one thing I don't know So when it comes to us, baby keep your mouth closed So don't talk (Don't talk, don't tell your friend) Oh, don't tell Don't tell, oh no I promise Be quiet be quiet ...oh hey Or I will just deny it Be quiet Don't talk Ohooo, between us Don't talk Don't tell your friends Yeah yeah Don't talk or this all will end I promise Be quiet, be quiet Or I will just deny it Be quiet, ssshh Keep it between us Keep your mouth closed

就像一个射击之星您为首的我你说出了我的心但不会设定Me免费哦男童你要耐心与我哦男童我想结识你好得多让我们从它列作为朋友，为一段时间你紧握我的眼睛当我第一次看到你的微笑哦男童这可能真的变成东西哦男童这就是为什么我不想急匆匆它不谈论不告诉你的朋友关于我们不谈论还是这一切将结束我承诺肃静，肃静或者我会否认安静， ssshh 保持我们之间我走出去，与一群我的朋友你能满足了，与我们在9点45分哦男童并不像有什么东西我们之间哦男童从现在起，让我们保持一份秘密不谈论不告诉你的朋友关于我们不谈论这个或将结束我承诺肃静，肃静或者我会否认肃静， sshh 保持我们之间我知道它觉得这是真正的但我需要一段时间来看看如果你立志成为一个对我来说从现在开始让我们只保持hush 让我们继续我们之间这是真正的原因我真的不小心 cuz人喜欢聊天，人们喜欢凝望只是一件事，我猜想，只是一件事我不知道因此，当谈到对我们来说，婴儿保持口腔封闭所以不要谈（不要说，不要告诉你的朋友）哦，不告诉他们不告诉他们，哦，没有，我答应肃静，肃静。。。

To meet the multilayer operation of electro-optical devices, the self-crosslinkable fluorinated 6F-PEEK-PEP, 3F-PEEK-PEP and 11F-PAE-PEP terminated with phenyl ethynyl moieties were prepared successfully.

3F-PEEK和6F-PEEK薄膜在光通讯波段具有两个有较低吸收的"窗口"，也就是说在光通讯波段有较小吸收。

与pep talk相关的网络解释 [注：此内容来源于网络，仅供参考]

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