partial antigen

partial antigen的基本解释

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不完全抗原

与partial antigen相关的网络例句 [注：此内容来源于网络，仅供参考]

In the absence of appropriate costimulation, partial activation of T cells can be lead to a state of long-lasting antigen-specific unresponsiveness or anergy.

在TCR识别MHC-抗原肽的同时，B7和CD28分子对的共刺激作用将使T细胞增殖并上调细胞因子表达，启动免疫应答反应。

The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因，并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列，通过对克隆的降钙素序列的比较研究，结果显示同一科的鱼降钙素序列保守性较高，同时，根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究，结果显示在分类学上，分类地位较远的鱼，其降钙素相似性较低，分类地位越接近的鱼，其降钙素相似性越高；我们利用谷胱甘肽S-转移酶(Glutathions S-transferase，GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究，应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索，并将纯化的融合蛋白作为抗原，获得了高效价的兔抗鲑鱼降钙素免疫血清；生物活性研究表明，大肠杆菌表达的融合蛋白具有显著的降血钙作用。

Results: The proliferation of Kasumi-l cell was obviously inhibited by VPA on dose- and time-dependent manner. The IC50 was 2.33 mmol/L treated with VPA for 72 h. Incubation with VPA, the cell ratio at G0/C1 phase increased. VPA induced myeloid cell differentiation antigen CD11b to increase with a time-and dose-dependent way and caused apoptosis. Wright's staining and light microscope were used to show the morphology of partial differentiation and obvious apoptosis with 3 mmol/L VPA for 72 hours. The typical DNA ladder of apoptosis characteristic was observed with DNA gel electrophoresis.

结果：VPA能显著抑制Kasumi-1细胞的增殖，且呈剂量和时间依赖性，VPA处理Kasumi-1细胞72h时的半数抑制浓度(IC50)为2.33 mmol/L；VPA处理后，细胞周期检测G0/G1期细胞比例逐渐增高；VPA能诱导髓系分化抗原CD11b表达水平的阳性率升高，呈时间及剂量依赖性；VPA能诱导Kasumi-1细胞凋亡，Kasumi-l细胞经VPA3 mmol/L处理72h后，出现典型的凋亡细胞所具有的梯形DNA条带。