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Different strains have different reaction to the delay of oviducts in body after animal death. After 10min delay C57BL/6 mouse oocytes have death rate of 56.5%,significantely higher than Kunming mouse (47.6%);Spontaneous activation rates were respectively 13.3% and 46.0%, C57BL/6 were obviously lower than Kunming mouse.4. The oviducts were obtained after being delayed 5min 24h after the mice were injected with hCG. The oocytes were cultured in CZB. About 81.1% occurred spontaneous activation, evidently lower than parthenogenetic rate (96.4%) with SrCl_2. Spontaneous activable oocytes had high cleavage rate(93.2%) and 4-cell rate(87.3%). However, spontaneous activable oocytes had blastula development rate(18.7%) as low as parthenogenetic oocytes by SrCl_2(22.9%).
不同品系小鼠卵母细胞对输卵管在体内滞留产生的反应不同,滞留10min C57BL/6系小鼠卵母细胞死亡率为56.5%,显著高于昆明鼠(47.6%);自发激活率分别为13.3%和46.0%,C57BL/6系小鼠显著低于昆明鼠。4.hCG后24h体内滞留5min卵母细胞在CZB中培养自发激活率为81.1%,显著低于SrCl_2孤雌激活率(96.4%);自发激活的卵母细胞有较高的卵裂率(93.2%)和4-cell比率(87.3%),但囊胚率(18.7%)较低,同卵龄的卵母细胞经SrCl_2孤雌激活囊胚发育率为22.9%,差异不显著。
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Results:There were 261 oviducts in the 135 infertile women and 57 of them were obstructed.The successful rate of tube insertion is 73.3% in the 57 obstructed oviducts.
结果:135例不孕症患者261条输卵管,经插管确诊梗阻输卵管57条,治疗成功率为733%。
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Methods: 60 oviducts and placental villi from tubal pregnant women were tested for the presence of HSV DNA by in situ hybridization. Additionally, 20 normal oviducts were used as control.
方法应用非放射性核酸分子原位杂交技术检测60例异位妊娠患者输卵管及绒毛组织HSV DNA的存在情况,并与正常输卵管比较。
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oviducts:输卵管
ocelli 单眼 | oviducts 输卵管 | oviposition 产卵