olfactory bulb
- olfactory bulb的基本解释
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[医] 嗅球
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METHODS: According to adherent + Thy1.1 antibody and complement-purification method, cranium was opened to expose olfactory bulb. Thereafter, two olfactory bulbs were obtained to remove cerebral pia mater, blood capillary, and peripheral tissues; additionally, olfactory nerve layer and olfactory bulb granular layer were sheared into 1-mm3 pieces for extract single-cell suspension. The cells were adjusted at the density of 1×107 /L and incubated with poly-l-lysine-coated culture bottle or culture plate in 5% CO2 incubator at 37 ℃. On the third day, cells were cultured with serum-free DMEM/F12 culture media.
在差速贴壁+Thy1.1抗体及补体纯化法的基础上,剪开大鼠颅骨,显露位于颅腔前方的嗅球,取出2只嗅球,在显微镜下去除嗅球表面的软脑膜和毛细血管及外周组织,保留富含嗅鞘细胞的嗅神经层和嗅球颗粒层,剪成1 mm3小块分离获取单细胞悬浮液,调整细胞密度至1×107 L-1,接种在用poly-l-lysine包被的培养瓶或培养板中,于37 ℃、体积分数为5%的CO2培养箱中培养,第3天用无血清DMEM/F12培养基换液培养。
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RESULTS: Olfactory bulb-derived olfactory ensheathing cells adhered to the wall at 24 hours. Olfactory mucosa-derived olfactory ensheathing cells adhered to the wall at 4-5 days. The morphology of the two kinds of cells was similar. Most of them were bipolar and spindle-shaped, and few were tripolar and flat.
结果:体外培养24 h嗅球源性嗅鞘细胞即可贴壁,而嗅黏膜源性嗅鞘细胞多在培养四五天后贴壁,两种来源的嗅鞘细胞形态相似,以双极或梭形细胞为主,少量为3极及多突起形多级细胞,同时夹杂扁平、煎鸡蛋形细胞。
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Study on the rapid Propagation Technique of Lycoris. Herb and get follow results: In the period found of axenic clone 0.1%HgCl2 is the best disinfector to deal with the Lycoris" bulb ,as to neat part, such as root, leave, and bulbil, is fit to use 0.1%HgCl2 antisepsis 7min. And found the best effect is the bulb scale with base. Root, leaf and bulb scale without base all were not inducement adventitious buds. Different position of bulb had different culture effect. 3-15 of middle part of bulb can be induced most adventitious buds but inner and outer of least it. Incised the bulb with three types (pieces of eight, pieces of six, piece of four), and found the type of pieces of six is best to Lycoris mass production. L.sprengeri is fit MS+BA5mg/l+NAA0.1mg/l and L.squamigera is fit MS+BA5mg/l+NAA0.5mg/l, but the medium fit to culture L.longituba haven"t be found.In the period of Subculture-Found of mass production, the most multiplication of adventitious buds in MS+BA5mg/l+NAA0.5mg/l during subculture of L.sprengeri, L.squamigera.
石蒜属植物快速繁殖技术研究中,在无菌无性系建立阶段:鳞茎以0.1%HgCl_2消毒10-12分钟效果最好,而叶、根尖、鳞芽等较干净部位选用0.1%HgCl_2消毒7分钟;用三种石蒜属植物的叶片、根尖、鳞芽及带基盘与不带基盘鳞片进行培养,以带基盘鳞片诱导分化效果最理想,鳞芽易培养出芽,但数量有限,而叶片、根尖与不带基盘鳞片均未诱导分化;带基盘鳞片为石蒜属植物快速繁殖最佳外植体,以鳞茎中部3-15层芽诱导率高,较外层稍次之,内部鳞片诱导率最低;选用八等分法、六等分法、四等分法切割鳞茎,六等分法综合效果最好;每外植体带三鳞片培养最为适宜;三种石蒜属植物各自适合的培养基成分不同,换锦花在MS+BA5mg/l+NAA0.1mg/l中培养效果最好,夏水仙为MS+BA5mg/l+NAA0.5mg/l,而白花长筒石蒜在各培养基组合中培养效果均不理想,其适合的培养条件有待于进一步研究。
- 更多网络解释 与olfactory bulb相关的网络解释 [注:此内容来源于网络,仅供参考]
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olfactory bulb:嗅球
...与Pais发现座狼鳄有以下特征:[[额骨]]中线有条深沟、[[嗅球]](Olfactory bulb)处有个凹陷、前上颌骨的最后牙齿加大,* 相对于它们的脑部,暴龙类具有大比例的[[嗅球]](Olfactory bulbs)与[[嗅神经|嗅觉神经]],这显示暴龙类具 ...b|暴龙的脑部模型,
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olfactory bulb:大脑嗅球
大脑中有两部分的生殖能力相当旺盛:齿状回区(dentate gyrus)的细胞和大脑嗅球(olfactory bulb). 图7显示的是老鼠的大脑嗅球的横截面,图中绿色的部分代表相较"年轻"的细胞,它们形成于动物的成人时期. 科学家们通过研究还发现--没有了新生细胞,
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olfactory bulb:[解剖] 嗅球
obtained score 实得分数 | olfactory bulb [解剖] 嗅球 | on the spot 现场
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olfactory bulb:嗅球,嗅脑
\\"嗅觉的\\",\\"olfactory\\" | \\"嗅球,嗅脑\\",\\"olfactory bulb\\" | \\"嗅觉皮膜\\",\\"olfactory epithelium\\"
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accessory olfactory bulb:犁鼻器
嗅觉器官:olfactory organ | 犁鼻器:accessory olfactory bulb | 人工嗅觉:artificial olfactory
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