mutation of thrushes
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YMDD mutation: HBV YMDD mutation were developed in 12 lamivudine recipients, whereas the treated group had no incidence of HBV YMDD variation, there were significant difference between two groups (p=0.000). YMDD mutation resulted in increase of the mean HBVDNA levels (p=0.000), but no contribution to serum ALT diversification (p>0.05). 2. Precore nt1896 mutation: Precore nt1896 mutation incidence had no difference between lamivudine recipients and objects who received combination therapy of Kidney-invigorating Granule and Marine Injection, and patients of HBeAg negative and HBeAg positive had a close incidence of precore mutation as well as. Precore nt1896 mutation had no influence on serum ALT level. The occurrence of precore nt1896 mutation contributed to serum HBVDNA load increased and worse response of HBVDNA extenuation. 3. HBV genotype: The baseline of serum HBVDNA gradually increase in turn of genotype B, genotype C and genotype B/C. Genotype B seemed to obtain higher rate of serum HBVDNA loss and HBeAg/anti-HBe seroconversion after combination therapy of Kidney-invigorating Granule and Marine Injection, differencing with lamivudine therapy.
疗效相关因素研究:1YMDD变异:对照组有12例检出YMDD变异,治疗组无1例检出,两组比较有显著性差异(P=0.000);发生YMDD变异者血清HBVDNA和ALT水平均有所升高,HBVDNA平均水平高于非变异者(P=0.000);血清ALT与非变异者无明显差异(P>0.05)。2前C区nt1896变异:前C区nt1896变异发生率在治疗组和对照组以及HBeAg阴性和HBeAg阳性患者之间均无明显差异(P>0.05);1896c变异对血清ALT水平无明显影响;治疗组发生1896c变异者,其HBVDNA平均水平较之未变异者明显升高(P<0.05),治疗后HBVDNA下降幅度明显低于未发生1896c变异者(P=0.000)。3HBV基因型:血清HBVDNA基础水平在B、C、B/C混合型间存在明显差异(P<0.05),按B型、C型、B/C混合型依次升高;治疗组HBVDNA阴转率、HBeAg/抗-HBe血清转换率,B型似乎高于C型和B/C混合型,但无统计学意义(P>0.05);对照组HBVDNA阴转、HBeAg/抗-HBe改变在B、C、B/C混合型间无明显差异(P>0.05)。
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The result of uroscopy of the proband was strong positive. There was a novel deletion mutation of c.876-877 del TC in the coding region of exon 6 of IDS gene, which was a hemizygous mutation. However, the mutation of his mother and sister was a heterozygous mutation. Detection of the exon 6 of IDS gene showed that the mutation was not found among normal controls and other patients with MPS I, IV, and VI other than MPS II. Homology comparison of amino acid sequences from different species showed that the phenylalanine glutamine of the mutation site of c.876-877 del TC located in p.292-293 was highly conserved. The activity of IDS enzyme of the proband was only 2.3 nmol/4 h/mL, which was much lower than normal; but the activity of IDS enzyme of his father, mother and sister was 641.9 nmol/4 h/mL, 95.8 nmol/4h/mL and 103.2 nmol/4h/mL, respectively.
结果显示:先证者尿检呈强阳性;其IDS基因exon 6编码区内存在c.876-877 del TC新缺失突变,为半合子突变,而其母、其姐为杂合突变;正常对照和其他非II型MPS患者的IDS基因exon 6的检测结果均未发现该突变;不同物种氨基酸序列的同源性比对显示: c.876-877 del TC突变所在的位置即p.292-293的苯丙氨酸谷氨酰胺高度保守;酶活性测定的结果显示:先证者的IDS酶活性仅为2.3 nmol/4 h/mL,大大低于正常值,而其父的为641.9 nmol/4 h/mL,其母的血浆酶活性为95.8 nmol/ 4h/mL,其姐的为103.2 nmol/4 h/mL。
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Studies on the mutant breeding of high-yield gibberellins strain The protoplast of Gibberella fujikuroi m978 were treated by mutation breeding forhigh-yield gibberellins, LiCl mutation was better than DES mutation or UV irradiation ifsingle mutation, but any single mutation was worse than compound mutation by whichthe mutants were more overproducting and genetically stable.
采用传统诱变育种,筛选高产菌株在原生质体条件下,对赤霉素产生菌m978的原生质体进行菌种选育;使用单因子诱变方法,LiCl处理的效果优于DES诱变和UV诱变;复合诱变比单因子诱变更容易得到高产突变株,且获得的高产突变株稳定性更优。
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a mutation of thrushes:一群畫眉鳥
a flight of swallows 一群燕子 | a mutation of thrushes 一群畫眉鳥 | an ambush of tigers 一群老虎