- 拼写相近词组、短语
- mus.
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The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.
结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达,且在未成熟卵母细胞中,MⅠ期的表达明显强于GV期的表达;②RT-PCR产物克隆测序结果显示:昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp~1153bp)]完全一致;Blastn比对结果发现:在不同物种中差异较小,选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析,提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%,100%,100%,36%,100%,100%,67%,100%,100%,92%,99%,99%,99%;Clustal 1.81和Treeview构建出的分子进化树表明:在上述14种生物中昆明小鼠与中国仓鼠进化关系最近,与兔子、猪聚成一簇,与食蟹猴进化关系最远;③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段,重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。
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It is fundamental condition: Benefit 810 thousand population, 970 thousand mus of farmland, mountain forest area 4.2 million mus, careless field 1.2 million mus, it is famous " the countryside of metasequoia "," the countryside of spinach "," the countryside of the rhizome of Chinese goldthread "," nicotian countryside ", the mineral products such as fossil of natural gas, bittern, coal, gesso, ancient biology is resourceful, development property has certain base.
081万人口,97万亩耕地,山林面积420万亩,草场120万亩,是有名的"水杉之乡"、"菠菜之乡"、"黄连之乡"、"烟草之乡",天然气、卤水、煤炭、石膏、古生物化石等矿产资源丰富,发展产业有一定基础。
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Wei Dong belongs to reason of ancient Yellow River to, it is sandy wasteland more sparsely, involve 5 rural area 273 villages, total land 703 thousand mus, its intertill ground 519 thousand mus, appropriate forest sandy wasteland 178 thousand mus, 16.1% what occupy entire county area about.
卫东属古黄河故道,多是沙荒薄地,涉及5个乡273个村,总土地70.3万亩,其中耕地51.9万亩,宜林沙荒17.8万亩,约占全县面积的16.1%。
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Mus caroli:柯氏小鼠
(23)丛林鼠 Mus famugus | (24)柯氏小鼠 Mus caroli | (25)柯氏小 鼠 Mus caroli
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Mus pahari:锡金小家鼠
(21)小家鼠 Mus musculus | (22)锡金小家鼠 Mus pahari | (23)丛林鼠 Mus famugus
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Mus musculus House mouse:家鼷鼠
56.田鼷鼠 Mus caroli Formosan mouse | 57.家鼷鼠 Mus musculus House mouse | ◎ 58.台湾高山白腹鼠 Niviventer culturatus Formosan white-bellied rat