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There was also not any haploid metaphases (n=16 or 19) was detected in hybrids. However, about 1-2% metaphases observed in this study was allopolyploid. GISH proved that they was made up of haploid genomes of M. nobilis (n=16) and triploid genomes of C. farreri (n=57). GISH was also employed to detect different chromosome components and identify donor chromatin in hybrids between C. farreri♀×A.
但我们检测到了约1-2%异源多倍体的分裂相,GISH分析后发现这些分裂相是由单倍的华贵栉孔扇贝的染色体和多倍的栉孔扇贝染色体组成,比如论文中图示了一个异源四倍体的分裂相,它是由单倍的华贵栉孔扇贝的染色体(n=16)和三倍的栉孔扇贝染色体(3n=57)组成。
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RESULTS: No Chromosome somatotype aberration was found in 100 metaphases in the experimental group.
结果:①每只实验动物观察100个中期分裂细胞,均未见染色体型畸变。
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It shows that As2O3 has marked cytogenetic toxic effect on the root tip cells of Vicia Faba,and effect of accumulating the metaphases in mitosis.
结论是As2O3对蚕豆根尖细胞具有明显的细胞遗传学毒性效应,并具有积累有丝分裂中期细胞的效应。
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The results showed that 4~8 Ag-NORs were observed in their metaphases respectively.
结果表明,在中期分裂相中各自的A g-NORs数在4-8个之间。
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Good chromosome preparation methods are necessary to get good spreading of chromosomes within metaphases for karyotype analysis.
染色体标本的制备是核型分析最基础的工作,要制作高质量的染色体标本,获得可靠的镜检结果,必须要有很好的制片方法。
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