messenger RNA
- messenger RNA的基本解释
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[化] 信使RNA
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Methods: One-step trizol had used to get the total RNA of the four animal brain, then PEGS000 to purify the small RNA from the total RNA, after the ligation ofpoly on the 3\' and the adaptor on the 5\', the oligo primer will be used to reverse translation the small RNA, and then used the specific primers to amplification the small RNA sequences. After the gel purition recovery, the small RNA will be ligated to the T-vector, and be transformed to the TOP 10 E. coli, at last, we picked up the masc. clones for sequencing.
通过Trizol一步法提取四种动物脑中的总RNA,接着用PEG纯化其中的小分子RNA,再经过poly连尾反应在小RNA的3'端连接上一段poly,然后在5'端连上adaptor,用Oligoprimer将连接好的小RNA进行反转录,用根据adaptor和Oligoprimer设计的引物来扩增小RNA,扩增后的小RNA进行胶回收后连接到T载体上,转化TOP10大肠杆菌后挑取阳性克隆进行测序,得到的序列再经过生物信息学的分析后确定是否为miRNA。
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RNA interference represents a common biological phenomenon of double-stranded RNA mediated post-transcriptional gene silencing, which lies in altitude plants and animals. The mechanism of this process is described as that: the dsRNA in cells are cut into double-stranded small interfering RNA. By joining with an effector complex termed RISC (RNA-induced silencing complex), siRNA binds to the cellular RNA with homologous sequences, and contributes to the degradation of the corresponding mRNA, the inhibition of specific gene expression.
RNAi技术是双链RNA(double strand RNA,dsRNA)介导的序列特异性的转录后基因沉默,广泛存在于高等植物和动物中,其机制是细胞内的dsRNA被Dicer剪切成双链的小分子干涉RNA (small interfering RNA,siRNA)与RNA诱导的基因沉默复合物结合,降解与之互补的靶mRNA,特异性抑制靶基因表达。
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At present it is very difficult to predict all the RNA secondary structures. This paper describes RNA secondary structure prediction methods from 1970\' to this day in a systematic way some. Tracing back and following the tracks of prediction methods the author made a summary of RNA research progress and situation while a enumeration problem of RNA secondary structure is studied with the method of combinatorial enumeration.
目前所有有关RNA二级结构的预测算法要预测出所有序列的二级结构仍然很困难,本文系统介绍了从上世纪70年代以来发展至今的各类RNA二级结构预测算法,从对RNA结构预测方法的追溯和跟踪,对当今RNA研究的进展和形势做了一个总结,并且应用组合计数的技巧解决了一类RNA二级结构的计数问题。
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messenger rna:信使
mesozoic era 中生代 | messenger rna 信使 rna | metabiosis 半共生
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messenger rna:信使核糖核酸
marine invertebrates 海洋无脊椎动物 | messenger RNA 信使核糖核酸 | meteor shower 流星雨
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messenger rna:传信息核 核酸
Melanoma 黑色素瘤 | Messenger RNA 传信息核 核酸 | Metastasis 转移
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messenger rna:信息核糖核酸
促性腺激素 gonadotropin | 信息核糖核酸 messenger RNA | 侵犯性 invasiveness
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mRNA messenger RNA:信使核糖核酸
mol mole 摩尔,"物质的量"单位 | mRNA messenger RNA信使核糖核酸 | McAb monoclonal antibody单克隆抗体
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