membranes
- membranes的基本解释
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n.
(动物或植物体内的)薄膜( membrane的名词复数 ), 隔膜, (可起防水、防风等作用的)膜状物
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Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.
发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合,而且结合是饱和性的、可被竞争的,符合配体-受体结合的典型特征,同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性,表明水稻细胞质膜上存在nsLTP的受体;利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜,再进行结合实验,结合活性几乎不受影响;而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失,表明其受体为没有经过糖基化修饰的蛋白质;利用交联剂BS~3交联配体一受体后,再进行SDS-PAGE分离和放射自显影,结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合,可能是其受体;细胞外碱化实验表明,nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应,却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应,表明nsLTP和Cryptogein结合细胞膜上相同的位点,保护了植物细胞免受Cryptogein导致的细胞程序性死亡,并诱导系统获得性抗性的产生;体外Pull-down实验表明,nsLTP144和水稻的OsCaM1具有相互作用,暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。
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Silk fibroin-chitosan blend membranes were prepared by mixing method and the mechanics of blend membranes was measured. The result shows that the blend membranes have good mechanical properties and can conquer the weakness of silk membranes, stiffness and brickleness.
采用共混法制备丝素-壳聚糖共混膜,并测定其力学性能,结果表明各种配比的丝素-壳聚糖共混膜均具有良好的机械性能,能够克服纯丝素膜刚而脆的弱点。
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In this thesis the planar bilayer lipid membranes inlaid with oxide cholesterol , phosphatidylcholine or cephalin have been prepared .The effects of membranes formulas on membranes stability and zinc ions transfer ability have also been studied.
中文摘要采用氧化胆固醇与磷脂混合作为成膜液制备双分子层脂质膜的方法,研究了制备平板双分子层脂质膜的配方和成膜条件,并对制得的双分子层脂质膜的稳定性、离子通透性、离子选择通过性能、双层膜中氧化胆固醇对通透性的影响等进行了研究。
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semipermeable membranes:半透膜
semipermanent storage structures半永久储存构架 | semipermeable membranes半透膜 | semipositive molds半溢式模
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Membranes:薄膜
在基布上复合的GORE-TEX薄膜(MEMBRANES)上有数以百万计的微孔. 对于水气水滴(其尺寸通常为1毫米数量级)而言,微孔(其尺寸通常为0.0001毫米数量级)的尺寸只有水气水滴的几千分之一,水气水滴无法透过微孔(即防水);
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Membranes:膜
在基布上复合的GORE-TEX薄膜(MEMBRANES)上有数以百万计的微孔. 对于水气水滴(其尺寸通常为1毫米数量级)而言,微孔(其尺寸通常为0.0001毫米数量级)的尺寸只有水气水滴的几千分之一,水气水滴无法透过微孔(即防水);
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Membranes:薄膜,细胞膜
Sweep:扫 | Membranes:薄膜,细胞膜 | Beacon:信号灯
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Membranes:過濾膜
過濾頭 - Syringe Filter | 過濾膜 - Membranes | 微量吸管 - Pipettor
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