- 拼写相近单词
- leptospirosis
- 更多网络例句与leptospira相关的网络例句 [注：此内容来源于网络，仅供参考]
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Methods Peritoneal macrophages of guinea pigs were infected in vitro by three different Leptospira strains, the virulent Leptospira interrogans serovar Lai type strain Lai, the avirulent L. interrogans serovar Lai type strain IPAV, and the nonpathogenic L. biflexa serovar Patoc type strain PatocⅠ, respectively, and heat inactivated Staphylococcus epidermidis was added 0.5, 1.5, 3 and 6 h after infection and incubated for 30 min. The effect of Leptospira on the phagocytosis of macrophage was evaluated by the inactivated Staphylococcus epidermidis phagocytosis rate and phagocytosis index. Phagocytosis and ultrastructure of peritoneal macrophages were observed by transmission electron microscopy 3 h after infection, and changes of cytoskeleton of the macrophages were observed by laser scanning confocal microscopy.
用三种不同毒力的钩体（致病性问号钩端螺旋体赖型有毒株Lai株、赖型无毒株IPAV株以及非致病性双曲钩端螺旋体Patoc型PatocⅠ株）分别感染体外培养的豚鼠腹腔巨噬细胞，并分别于感染后0.5、1.5、3和6 h加入热灭活表皮葡萄球菌孵育30 min，通过计算巨噬细胞对灭活表皮葡萄球菌的吞噬率和吞噬指数，检测钩体对巨噬细胞吞噬功能的影响；感染后3 h透射电镜观察巨噬细胞对钩体的吞噬、降解和细胞超微结构的变化；用激光共聚焦显微镜观察细胞对钩体的吞噬和巨噬细胞细胞骨架的变化。
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Objective To clone the flagellar biosynthesis genes fihA, flhB2 and fliR of Leptospira interrogans and construct their prokaryotic expression system Methods Extract genomic DNA from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai strain 56601 by phenol-chloroform method and amplify fihA, flhB2 and fliR gene fragments by high fidelity PCR.
目的 克隆问号钩端螺旋体鞭毛相关基因flhA、flhB2和fliR，并构建其原核表达载体。
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Methods Two sets of primers were derived from an inserted sequence IS1533 of Leptospira borgpetersenii.
方法选 Leptospira borgpetersenii 中的一段保守序列 IS1533，设计两对引物，用套式 PCR 检测六种不同血清型的钩体。
- 更多网络解释与leptospira相关的网络解释 [注：此内容来源于网络，仅供参考]
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Leptospira canicola：犬<>端螺旋属
牛<>端螺旋体 Leptospira bovis | 犬<>端螺旋属 Leptospira canicola | 感冒伤寒性<>端螺旋体 Leptospira grippotyphosa
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Leptospira mini：小螺旋体
出血性黄疸螺旋体 Leptospira icterohaemorrhagiae | 小螺旋体 Leptospira mini | 波莫纳<>端螺旋体 Leptospira pomona
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Leptospira autumnalis：秋令<>端螺旋体
澳洲<>端螺旋体 Leptospira australis | 秋令<>端螺旋体 Leptospira autumnalis | 牛<>端螺旋体 Leptospira bovis