lay into
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以言词或拳脚攻击
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痛打(反复打击)
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The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.
本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法,研究丘脑网状核(nucleus reticularis thalami,RT)中γ-氨基丁酸(gamma-amino butyric acid ,GABA)能神经元、一氧化氮(nitrogen monoxidum,NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate,cAMP)及中缝背核(nucleus raphes dorsalis,DRN)至RT的5-羟色胺(5-hydroxytryptamine,5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP,5μg),注射当天睡眠时间略有减少,第二日睡眠时间显著减少,觉醒时间明显增多,第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后,与生理盐水组比较,睡眠时间增加,觉醒时间减少;而RT内微量注射L-谷氨酸(glutamic acid, L-Glu, 0.2μg)后,睡眠时间减少,觉醒时间增加;RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline,BIC,1.0μg)后,睡眠时间减少,觉醒时间增加;RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen,BAC,1.0μg)后,产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg,0.5μg)后,与生理盐水组对比,睡眠时间略有减少,但无显著性意义;而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside,SNP,0.2μg)后可明显增加觉醒时间,缩短睡眠时间;微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine,L-NNA,2.0μg)后,引起睡眠时间增多,觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用;RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后,与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠,其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate,MOR,1.0μg)后与生理盐水组对比,睡眠时间减少而觉醒时间增加; RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride,NAL,1.0μg)后与生理盐水组比较,睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后,与生理盐水组对比,原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较,睡眠时间增多而觉醒时间减少;RT内微量注射亚甲蓝(methylene blue,MB,1.0μg)后,与NS组比较,睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg),同时在双侧RT内微量注射NS (1.0μg)后,与对照组(DRN和双侧RT注射NS, 0.2μg)比较,睡眠时间减少,觉醒时间增多;大鼠DRN内微量注射L-Glu(0.2μg),同时在双侧RT内微量注射二甲基麦角新碱(methysergide, MS, 1.0μg )后,与对照组(DRN注射L-Glu 0.2μg,双侧RT注射NS 1.0μg)比较,睡眠时间增多,觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine,PCPA,10μg),同时在双侧RT内微量注射NS (1.0μg)后,与对照组(DRN和双侧RT注射NS, 1.0μg)比较,睡眠时间增多,觉醒时间减少;大鼠DRN内微量注射PCPA(10μg),产生睡眠增多效应后,在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan , 5-HTP, 1.0μg )后,与对照组(DRN注射PCPA 10μg,双侧RT注射NS 1.0μg)比较,睡眠时间减少,觉醒时间增多。
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3At first discharge port, lay time shall be commenced at 13:00 if Notice of Readiness is tendered before noon and at 8:00 next day if NOR is given in the afternoon. Time lost in waiting for berth hall be counted as lay time. Time used for shifting the vessel from place of stoppage, lay-by berthto discharge berth, and lighterage/lightening shall be counted as lay time. At second discharge port/lay time shall be commenced to count immediately upon vessel arrival.
在第一个卸货港,如果准备就绪通知在中午前发出,正式卸货时间为13:00,如果准备就绪通知在头天下午发出,正式卸货时间为次日的8:00,在码头上等待时间视为卸货时间,其他如紧急码头停泊等视为卸货时间,在第二个卸货港,船一旦到达开始计算卸货时间。
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A little after Noon I found the Sea very calm, and the Tyde ebb'd so far out, that I could come within a Quarter of a Mile of the Ship; and here I found a fresh renewing of my Grief, for I saw evidently, that if we had kept on board, we had been all safe, that is to say, we had all got safe on Shore, and I had not been so miserable as to be left entirely destitute of all Comfort and Company, as I now was; this forc'd Tears from my Eyes again, but as there was little Relief in that, I resolv'd, if possible, to get to the Ship, so I pull'd off my Clothes, for the Weather was hot to Extremity, and took the Water, but when I came to the Ship, my Difficulty was still greater to know how to get on board, for as she lay a ground, and high out of the Water, there was nothing within my Reach to lay hold of; I swam round her twice, and the second Time I spy'd a small Piece of a Rope, which I wonder'd I did not see at first, hang down by the Fore-Chains so low, as that with great Difficulty I got hold of it, and by the help of that Rope, got up into the Forecastle of the Ship; here I found that the Ship was bulg'd, and had a great deal of Water in her Hold, but that she lay so on the Side of a Bank of hard Sand, or rather Earth, that her Stern lay lifted up upon the Bank, and her Head low almost to the Water; by this Means all her Quarter was free, and all that was in that Part was dry; for you may be sure my first Work was to search and to see what was spoil'd and what was free; and first I found that all the Ship's Provisions were dry and untouch'd by the Water, and being very well dispos'd to eat, I went to the Bread-room and fill'd my Pockets with Bisket, and eat it as I went about other things, for I had no time to lose; I also found some Rum in the great Cabbin, of which I took a large Dram, and which I had indeed need enough of to spirit me for what was before me: Now I wanted nothing but a Boat to furnish my self with many things which I forsaw would be very necessary to me.
午后不久,海面风平浪静,潮水也已远远退去。我只要走下海岸,泅上几十米,即可到达大船。这时,我心里不禁又难过起来。因为我想到,倘若昨天我们全船的人不下小艇,仍然留在大船上,大家必定会平安无事。这时就可安抵陆地;我也不会像现在这样,孤苦伶仃孑然一身了。而现在,我既无乐趣,又无伴侣。想到这里,我忍不住流下泪来。可是,现在悲伤于事无济,我即决定只要可能就先上船去。当时,天气炎热,我便脱掉衣服,跳下水去。可是,当我泅到船边时,却没法上去,因为船已搁浅,故离水面很高;我两臂所及,没有任何可以抓住的东西。我绕船游了两圈,忽然发现一根很短的绳子。我惊异自己先前竟没有看见这根绳子。那绳子从船头上挂下来,绳头接近水面;我毫不费力地抓住绳子往上攀登,进入了船上的前舱。上去后发现船已漏水,舱底进满了水。因为船搁浅在一片坚硬的沙滩上,船尾上翘,船头几乎都浸在水里,所以船的后半截没有进水。可以想像,我急于要查看一下哪些东西已损坏,哪些东西还完好无损。首先,我发现船上的粮食都还干燥无恙。这时,我当然先要吃些东西,就走到面包房去,把饼干装满了自己的衣袋,同时边吃边干其他活儿,因为我必须抓紧时间才行。我又在大舱里找到了一些甘蔗酒,就喝了一大杯。此时此刻,我极需喝点酒提提神。我这时只想有一只小船,把我认为将来需要的东西,统统运到岸上去。
- 更多网络解释 与lay into相关的网络解释 [注:此内容来源于网络,仅供参考]
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lay into:以言词攻击
lay in 贮藏 | lay into 以言词攻击 | lay it on thick 过分责备
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lay into sth or sb:痛打
know a thing or two有所了解 | lay into sth or sb痛打 | learn a thing or two学到一点
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You lay your head into my lap:你将头埋入我腿间
You lay your head into my lap 你将头埋入我腿间 | I strike your face 我扇了你一耳光 | The taxi rushes through the night 的士整夜奔跑
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I lay down and fell into a dream:我躺在上面,掉进了一个梦
On the grass so green 青草,如此之绿 | I lay down and fell into a dream 我躺在上面,掉进了一个梦 | By a strange mellow sound 一个奇妙而圆润的声音传来
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I lay down and fell into a dream:我坠入梦境
By an old drinking well on the grass so green 依偎着绿草地上的那口枯井 | I lay down and fell into a dream 我坠入梦境 | By a strange mellow sound from a silver eyed bird 在银眼鸟那奇妙动人的歌声中
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