lane 10
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Thress homologous chromosomes of trisomics associated as a trivalent or a bivalent and a univalent or three univalent during diakinesis and metaphase 1 ;Laggard chromosome and univalent separated in advance at anaphase I ,leading to distribution of 10-1-10,9-12,11-11,10-2-10,10-12,10-10,9-1-11 besides 10-ll;And there was distribution of 11-11-10-10, 10-10-10-12, 10-10-10-11, 10-11-11-11, 11-11-10-9, 10-10-10-10, 9-12-10-10, 9-9-11-11, 11-10-10-9, 12-10-9-11, 11-9-9-13, 10-10-10-9 at anaphase II ;n l microspore frequency were different of four trisomics.
三体在终变期和中期Ⅰ,3个同源染色体联会成三价体,或2个联会成二价体和1个单价体,或3个都为单价体;后期Ⅰ出现落后染色体或单价体提前分离,导致除10-11式分离外,还有10-1-10,9-12,11-11,10-2-10,10-12,10-10,9-1-11等不均衡分离方式;后期Ⅱ有11-11-10-10,10-10-10-12,10-10-10-11,10-11-11-11,11-11-10-9,10-10-10-10,9-12-10-10,9-9-11-11,11-10-10-9,12-10-9-11,11-9-9-13,10-10-10-9等分离方式;4个三体中的n 1小孢子频率不同。
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Fig 1 Negative control Female umbilical cord sample has no Y-specific signal after in situ hybridization with the biotinylated Y-repeated sequence DNA probe PY3.4 Fig 2 Positive control Flow-sorted male umbilical cord cells hybridized to Y-specific DNA probe PY3.4,Every cell contains a Y-specific signal Fig 3 Fetal cells sorted from maternal blood Flow-sorted cells from a pregnant woman at 20 weeks of gestation hybridized to Y-specific DNA probe PY3.4,containing a Y-specific signal Fig 4 The result of polymerase chain reaction Lane 1:male umbilical cord NRBCs sorted by FITC-conjugated anti-monoclonal glycophorin A;Lane 2:sample of 2;Lane 3:male umbilical cord NRBCs sorted by FITC-conjugated anti-CD36;Lanes 4-5:samples of 1 and 3;Lane 6:50 cells of male;Lane 7:5 cells of male;Lane 8:200ng male DNA(positive+control);Lane 9:nonpregnant female DNA(negative+control);Lane 10:ΦX174 HaeⅢ Maker,271bp:amplified band of Y-specific gene SRY;383bp:amplified band of human β-globin gene
图1 阴性对照女性脐带血标本,经与生物素标记的Y-染色体重复序列DNA探针原位杂交后未见Y-染色体特异信号图2 阳性对照分选的男性脐血细胞经与Y-特异DNA探针杂交后,每一细胞都含有Y-染色体特异信号图3 母体外周血中分选的胎儿细胞从一位妊娠20周的孕妇外周血中分选出的细胞经与Y-特异DNA探针杂交细胞中含有Y-染色体特异信号图4 聚合酶链反应结果 1:GPA-FITC单抗分选男胎脐血NRBCs;2:2号标本;3:CD36-FITC单抗分选男胎脐血NRBCs;4、5:1、3号标本;6:50个男性细胞标本;7:5个男性细胞标本;8:200ng男性DNA;9:未孕女性DNA;10:ΦX174 HaeⅢ标准,271bp:为SRY基因扩增带;383bp:为人β-珠蛋白基因扩增带内参照
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The linear ranges of determination for pyrogallol, phloroglucinol, pyrocatechol, resorcinol and parodioxybenzene were 1.0×10〓~2.0 ×10〓mol l〓, 1.0×10〓~1.0×10〓mol l〓, 1.0×10〓~6.0×10〓mol l〓, 1.0 ×10〓~2.0×10〓mol l〓 and 1.0×10〓~6.0×10〓mol l〓 respectively, and their detection limits were 7.2×10〓mol l〓, 6.8×10〓mol l-1, 2.1 × 10〓mol l〓, 7.1×10〓 mol l〓 and 2.2×10〓mol l〓 respectively.
该方法测定连苯三酚、间苯三酚、邻苯二酚、间苯二酚和对苯二酚的线性范围分别为1.0×10〓~2.0×10〓mol l〓,1.0×10〓~1.0×10〓 mol l〓,1.0×10〓~6.0×10〓mol l〓,1.0×10〓~2.0×10〓mol l〓和1.0×10〓~6.0×10〓mol l〓;检出限分别为7.2×10〓mol l〓、6.8×10〓 mol l〓、2.1×10〓 mol l〓、7.1×10〓mol l〓和2.2×10〓mol l〓。
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