investigate in secret

investigate in secret的基本解释

-

暗访

与investigate in secret相关的网络例句 [注：此内容来源于网络，仅供参考]

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

As the colastantdevelopment of market economy and our country join WTO,only depend on this simple clause of the newly—increased crime of infringing upon business secret in criminal law of 1997 insufficient in an all-round way attacking criming effectively,and clause this have a great deal of perfect spaces,this thesis explains,analyses from such respects as the concept of the business secret and the composition important documents and asserting and punishing of the characteristic,crime of infringing upon business secret,etc.,pointing out the deficiency that the crime of infringing upon business secret exists and putting forward thc criminal legal system idea of perfecting the crime of infringing upon business secret.

随着市场经济的不断发展以及我国加入WTO，仅靠1997年《刑法》中增设的侵犯商业秘密罪这一简单条款，不足以全面、有效打击犯罪，并且该条款存在诸多不完善之处。从商业秘密的概念和特征、侵犯商业秘密罪的构成要件和认定及其处罚等方面进行阐述、分析，指出了侵犯商业秘密罪的刑事法制存在的不足，并提出了完善侵犯商业秘密罪的刑事法制构想。

How when we are in a Doubt or Hesitation, whether to go this Way, or that Way, a secret Hint shall direct us this Way, when we intended to go that Way; nay, when Sense, our own Inclination, and perhaps Business has call'd to go the other Way, yet a strange Impression upon the Mind, from we know not what Springs, and by we know not what Power, shall over-rule us to go this Way; and it shall afterwards appear, that had we gone that Way which we should have gone, and even to our Imagination ought to have gone, we should have been ruin'd and lost: Upon these, and many like Reflections, I afterwards made it a certain Rule with me, That whenever I found those secret Hints, or pressings of my Mind, to doing, or not doing any Thing that presented; or to going this Way, or that Way, I never fail'd to obey the secret Dictate; though I knew no other Reason for it, than that such a Pressure, or such a Hint hung upon my Mind: I could give many Examples of the Success of this Conduct in the Course of my Life; but more especially in the latter Part of my inhabiting this unhappy Island; besides many Occasions which it is very likely I might have taken Notice of, if I had seen with the same Eyes then, that I saw with now: But 'tis never too late to be wise; and I cannot but advise all considering Men, whose Lives are attended with such extraordinary Incidents as mine, or even though not so extraordinary, not to slight such secret Intimations of Providence, let them come from what invisible Intelligence they will, that' I shall not discuss, and perhaps cannot account for; but certainly they are a Proof of the Converse of Spirits, and the secret Communication between those embody'd, and those unembody'd; and such a Proof as can never be withstood: Of which I shall have Occasion to give some very remarkable Instances, in the Remainder of my solitary Residence in this dismal Place.

有时，我们会陷入无所适从的境地，踌躇不定不知道该走哪条路才好。这时候，内心常常会出现一种暗示，指示我们走这条路，虽然我们原来想走的是那条路。不仅如此，有时我们的感觉、愿望、或我们的任务明明要我们走那条路，可是心里忽然灵机一动，要我们走这条路；这种灵机也不知道是从哪里来的，也不知道出自什么影响，可就是压倒了原来的一切感觉和愿望，使我们走这条路。结果，后来的事实证明，如果我们当初走了我们自己想走的路，或者走了我们心目中认为应该走的路，我们则早已陷于万劫不复的境地。反复思索之后，我自己定下了一条规矩：每当自己心里出现这种神秘的暗示或冲动，指示我应做什么或不应做什么，我就坚决服从这种神秘的指示，尽管我不知道为什么该这么做或该这么走，我知道的只是心里的这种暗示或冲动。在我一生中，可以找出许许多多这样的例子，由于我遵循了这种暗示或冲动而获得了成功，尤其是我流落到这个倒霉的荒岛上以后的生活，更证明了这一点。此外还有许多例子。当时我若能用现在的眼光去看待，是一定会意识到的。但是，世上有许多道理，只要有一天能大彻大悟，就不算太晚。我奉劝那些三思而后行的人，如果在他们的生活里，也像我一样充满了种种出乎寻常的变故，或者即使没有什么出乎寻常的变故，都千万不要忽视这种上天的启示，不管这种启示是什么看不见的神明发出的。关于这一点，我不准备在这里讨论，也无法加以阐明。但这种启示至少可以证明，精神与精神之间是可以交往的，有形的事物和无形的事物之间是有神秘的沟通的。而且，这种证明是永远无法推翻的。关于这一点，我将用我后半生的孤寂生活中一些很重要的例子加以证明。

与investigate in secret相关的网络解释 [注：此内容来源于网络，仅供参考]

Raul：劳尔

西 前锋 7 劳尔(Raul) 西班牙 77.06.27 皇马B队 17 范尼() 荷兰(E) 76.07.01 曼联 20 伊瓜因(ín) 阿根廷(E) 87.12.10 河床 9 萨维奥拉() 阿根廷 81.12.11 巴塞罗那 2006-2007赛季西班牙甲级联赛:皇家马德里 成立时间:1902年 上赛季名次:第2名 主场:伯纳乌 (78500人) 主席:卡尔德隆() 主帅:卡佩罗(),

a secret door in the wall：墙上的一扇暗门

secret society 秘密社团 | a secret door in the wall 墙上的一扇暗门 | the secret of success 成功的秘诀

secret ballot：无记名投票

secret as the grave 守口如瓶的 | secret ballot 无记名投票 | secret ink 隐显墨水