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Chapter 1 is a preface to introduce the motives of this paper research methods scope restriction and frame Chapter 2 will fist introduce the concept of database and then discuss the necessity of database protection in the creating methods and with or without originality This chapter will put emphasis on the importance of originality to database Chapter 3 will observe the British America and Germany's viewpoint of originality to discuss their legislative mode of database legal protection Chapter will introduce EU Directive/96/9/EC of database protection and four judges of EU Court after the Directive takes effect in 2004 to find how the ECJ use the Directive Then according to the DG Internal Market and Service Working Pater and BEUC's evaluation of the Directive this paper will find whether there is positive reaction and influence in database market after EU member countries take the same legislations with the Directive Section 5 will introduce the legislation of British and Germany following the Directive and analyze the development and questions of the two countries' current legislation Chapter 5 will start with Americas case Feist v Jane Doe to understand the case's influence to American legal protection of databases and introduce the Parliament's proposal after the EU Directive Chapter 6 will introduce Japan legislation regarding legal protection of database works and discuss the goodness and badness through Japan academy and judicial viewpoint Chapter 7 will introduce Taiwan's legislation to find whether current law provides enough and adequate protection to databases and discuss whether there is a necessity of amendment At last this paper will try to provide some suggestions about our legislation Chapter 8 is the conclusion of this paper

第二章首先介绍资料库之概念，并以建置方式与是否具原创性区分方式探讨资料库法律保护之必要性，而因著眼原创性对於资料库法律保护之重要性，便於第三章以比较法独立观察英、美、德国对原创性之观点，以接续探讨三国对资料库所采取之立法途径。第四章部份介绍欧盟资料库保护指令之相关规定，并就指令公布后，欧盟法院於2004年接续作出四个关於资料库保护之判决加以介绍，以期寻求欧盟法院如何运用指令规范中之构成要件，而后并参考欧盟委员会工作小组及消费者组织报告对欧体资料库指令之评估文献，探究欧盟各国采取与指令相同立法后，在资料库市场上是否有正面的回应及影响，并於第五节针对英、德两国跟进欧盟资料库保护指令立法之介绍，并归纳、检讨英、德两国之现行法制之发展与所面临之问题。第五章延续第三章、第四章论述后，进入美国1991年Feist案后，美国法院对国内资料库保护之后续见解，探究1991年Feist案对美国资料库保护之影响程度，同时并述美国於欧体公布资料库指令后国会提出之资料库立法草案。第六章则针对日本新增资料库著作之立法模式加以介绍，并藉由日本学界、实务之见解探讨该立法模式之优缺点，再於第七章进入我国法制之介绍，以观察我国目前之法制是否提供资料库充分的保护，探讨资料库立法之必要性，最后尝试提出我国立法模式之建议。第八章则为总结之论述。

The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.

本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法，研究丘脑网状核(nucleus reticularis thalami，RT)中γ-氨基丁酸(gamma-amino butyric acid ，GABA)能神经元、一氧化氮(nitrogen monoxidum，NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate，cAMP)及中缝背核(nucleus raphes dorsalis，DRN)至RT的5-羟色胺(5-hydroxytryptamine，5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP，5μg)，注射当天睡眠时间略有减少，第二日睡眠时间显著减少，觉醒时间明显增多，第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后，与生理盐水组比较，睡眠时间增加，觉醒时间减少；而RT内微量注射L-谷氨酸(glutamic acid， L-Glu， 0.2μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline，BIC，1.0μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen，BAC，1.0μg)后，产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg，0.5μg)后，与生理盐水组对比，睡眠时间略有减少，但无显著性意义；而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside，SNP，0.2μg)后可明显增加觉醒时间，缩短睡眠时间；微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine，L-NNA，2.0μg)后，引起睡眠时间增多，觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用；RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后，与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠，其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate，MOR，1.0μg)后与生理盐水组对比，睡眠时间减少而觉醒时间增加； RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride，NAL，1.0μg)后与生理盐水组比较，睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后，与生理盐水组对比，原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较，睡眠时间增多而觉醒时间减少；RT内微量注射亚甲蓝(methylene blue，MB，1.0μg)后，与NS组比较，睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 0.2μg)比较，睡眠时间减少，觉醒时间增多；大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射二甲基麦角新碱(methysergide， MS， 1.0μg )后，与对照组(DRN注射L-Glu 0.2μg，双侧RT注射NS 1.0μg)比较，睡眠时间增多，觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine，PCPA，10μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 1.0μg)比较，睡眠时间增多，觉醒时间减少；大鼠DRN内微量注射PCPA(10μg)，产生睡眠增多效应后，在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan ， 5-HTP， 1.0μg )后，与对照组(DRN注射PCPA 10μg，双侧RT注射NS 1.0μg)比较，睡眠时间减少，觉醒时间增多。

On this basis, according to historical data, apply ANN and differential simulation method to get the quantitatively correlative relations between each production and its own influence factors, and introduce the new methods of prediction for dynamic indexes with gas-field development (The combinatorial prediction method based on fuzzy comprehensive evaluation, the method of ANN to select optimally combinatorial prediction models and the ANN prediction method based on genetic algorithm).(2) Base on mathematical programming, combine with quantitative economics and techno-economics, introduce economical indexes to establish production"s distribution optimal model, production"s constitution optimal model and measured production"s constitution optimal model, including multi-objective models and five-years models. Upon this, the optimal project for all gas field and each gas-collected factory can be got. Also, introduce the time value of capitals to improve on these models.(3) Base on the optimal solution theory and algorithm theory for the nonlinear programming problem, introduce the SUMT algorithm and genetic algorithm to study how to solve the models, and on the basis of normal genetic algorithm, make use of auto-adaptively modulating method to improve on normal genetic algorithm; Base on algorithm"s convergence theory and calculation"s complexity theory to analyze seriatim SUMT algorithm"s convergence and genetic algorithms convergence, and compare performance with each other.

在此基础上，利用神经网络方法和微分模拟方法根据历史数据得到各分项产量与其影响因素之间的定量关联关系，并引入气田开发动态指标新的预测方法(基于模糊综合评判的组合预测方法、神经网络优选组合预测模型预测方法以及基于遗传优化的神经网络预测方法)；(2)以数学规划为基础，结合数量经济学和技术经济学，引入经济指标建立产量分配优化模型、产量构成优化模型、措施产量构成优化模型、气田开发多目标规划模型以及五年规划模型，进而获得全气田及各采气厂的最优方案，并引入资金时间价值对五年规划模型进行改进；(3)以非线性规划问题的最优解及算法理论为基础，引入SUMT算法以及遗传算法对模型的求解进行研究，并在原有的遗传算法基础上，引入自适应调整方法对遗传算法进行改进；以算法的收敛性理论和计算复杂性理论为基础，逐一分析SUMT算法以及遗传算法的收敛性，并比较三种算法的优劣性。

与introduce into相关的网络解释 [注：此内容来源于网络，仅供参考]

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